Optimization of process parameters for xylanase production by Bacillus sp. in submerged fermentation

Abstract

In this study an attempt was made to optimize the cultural and nutritional conditions for xylanase production by Bacillus species in submerge fermentation process. Whole fermentation process was carried out in 250 ml Erlenmeyer flask with agitation speed of 140 rpm. Bacillus subtilis exhibit maximum xylanase production at initial medium pH of 8, substrate concentration of 2% with inoculum size of 2% at 35 C for 48 h of fermentation period. Further supplementation of sucrose, (NH 4) 2 SO 4 and peptone as a carbon and nitrogen sources favored enzyme production. The other strain Bacillus megaterium showed its peak xylanase production at initial medium pH of 8, inoculum size of 1.5% with substrate concentration of 1.5% at incubation temperature of 40 C for 72 h of fermentation period. The best carbon and nitrogen sources are xylose, KNO 3 and malt extract. Both strains can also utilize molasses at 0.5% concentration for xylanase production can grow in medium containing 0.2% NaCl (B. subtilis BS04) and 0.8% NaCl (B. megaterium BM07) respectively. The optimum temperature of xylanase was 50 C and pH was 5 and 5.5 by B. subtilis BS04 and B. megaterium BM07 respectively.

Fermenting rice bran as a carbon source for biofloc technology improved the water quality, growth, feeding efficiencies, and biochemical composition of African catfish Clarias gariepinus juveniles

“…Bacillus spp., greatly depend on the amount of available oxygen (Nadeem, Qazi, & Baig, 2009 | 3697 2011; Irfan, Asghar, Nadeem, Nelofer, & Syed, 2016). This seems to support the lower crude fibre content in the FerRB.…”

“…Production of starch degrading enzymes such as α ‐amylase and xylanase was enhanced in Bacillus spp. under anaerobic conditions (Divakaran, Chandran, & Pratap, ; Irfan, Asghar, Nadeem, Nelofer, & Syed, ). This seems to support the lower crude fibre content in the FerRB.…”

Production and Optimization of Xylanase Enzyme from Bacillus subtilis using Agricultural Wastes by Solid State Fermentation

“…Any other carbon sources like fructose, glucose, CMC (carboxymethyl cellulose) and arabinose were produce the less enzyme production. 24 Azeri et al reported that different strains of Bacillus sp. and all exhibit maximum xylanase production by using birch wood xylan as a carbon source.…”

Endoglucanase and Xylanase Production by Chryseobacterium Species Isolated from Decaying Biomass

“…For the production of cellulase by Bacillus subtilis and Bacillus circulans, pH in the range of 7.0-7.5 was found to be optimum by Ray et al [59]. According to the report of Irfan et al [43], pH 8 was the best among the pH values of the medium for xylanase production by B. subtilis BS04 and B. megaterium BM07 in submerged fermentation. In addition, lower pH, i.e., 4 and higher pH, i.e., 10 of the medium retards xylanase secretion drastically.…”

“…The optimum temperature for enzyme production by an organism may vary since it is likely to affect growth of the organism. Irfan et al [43] reported that the three bacterial strains used in the study did not grow well at 25ºC and thus resulted in decreased enzyme production. B. subtilis BS04 showed highest enzyme production at 35ºC.…”