Optimization of process parameters for the development of a cheese analogue from pigeon pea (Cajanus cajan) and soy milk using response surface methodology

Abstract

Response surface methodology was used to optimize starter culture concentration (3–7%), pigeon pea–soy solids ratio (1 : 0.3–1 : 1.1), pigeon pea–water ratio (1 : 12–1 : 9), incubation temperature (37–45C) and coagulation temperature (75–95C) in the development of a cheese analogue from pigeon pea and soy milk. Pigeon pea milk and soy milk were prepared by a hot water grinding method, followed by filtration and inoculation. Cheese analogue was obtained through coagulation of the fermented milk by boiling for 15 min, followed by filtration using double‐layered muslin cloth and pressing at 1 kg/cm 2 pressure for 1 h. The results showed that the total solids recovery (TSR), protein content, fat content and pH varied from 51.09–62.61%, 40.04–55.93%, 9.35–15.36% and 4.48–4.9, respectively, depending on the experimental conditions. The optimum conditions of process parameters for maximum total solids recovery were 6.0% starter culture concentration, 1 : 0.4 pigeon pea–soy solids ratio, 1 : 11 pigeon pea–water ratio, 40.5C incubation temperature and 88.5C coagulation temperature.

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Statistical analysis and optimization of nitrogen, phosphorus, and inoculum concentrations for the biodegradation of petroleum hydrocarbons by response surface methodology

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