2024
DOI: 10.1186/s13007-024-01180-9
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Optimization of preparation conditions for Salsola laricifolia protoplasts using response surface methodology and artificial neural network modeling

Hao Guo,
Yuxin Xi,
Kuerban Guzailinuer
et al.

Abstract: Background Salsola laricifolia is a typical C3–C4 typical desert plant, belonging to the family Amaranthaceae. An efficient single-cell system is crucial to study the gene function of this plant. In this study, we optimized the experimental conditions by using Box-Behnken experimental design and Response Surface Methodology (RSM)-Artificial Neural Network (ANN) model based on the previous studies. Results Among the 17 experiment groups designed by … Show more

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