Optimization of Pre-Treatment Process Parameters to Generate Biodiesel from Microalga

Abstract

Cell disruption is an integral part of microalga production process, which improves the release of intracellular products that are essential for biofuel production. In this work, pre-treatment parameters that will enhance the efficiency of lipid production using high-pressure homogenizer on microalgae biomass will be investigated. The high-pressure homogenizer that is considered is a GYB40-10S/GY60-6S; with a pre-treatment pressure of 1000 psi, 2000 psi, and 3000 psi, the number of passes; 1, 2, and 3, a reaction time of 3, 3.5, and 4 h. Pressure and cavitation increase the efficiency of the pre-treatment process of the homogenizer. In addition, homogenization shear force and pressure are the basic significant factors that enhance the efficiency of microalgae cell rupture. Also, the use of modelling to simulate pre-treatment processes (Response Surface Methodology (RSM), Box-Behnken Designs (BBD), and design of experiment (DOE) for process optimization will be adopted in this study. The results clearly demonstrate that high-pressure homogenization pre-treatment can effectively disrupt microalga cell walls to enhance lipid recovery efficiency, with a relatively short extraction time, both that are essential for maintaining a good quality of lipids for biofuel production. A maximum of 18% lipid yields were obtained after 3 h of HPH pre-treatment at 3000 psi.

Optimization of Biodiesel Production Using Nanomagnetic CaO-Based Catalysts with Subcritical Methanol Transesterification of Rubber Seed Oil

“…Response Surface Methodology (RSM) with Box-Behnken experimental design have been effectively applied to determined optimum conditions. Mathematical and statistical methods have been used to predict the effect of input process parameters based on the output responses [32][33][34]. The three parameters that most influence biodiesel production are: methanol/oil ratio, catalyst loading, and reaction time [35,36].…”

Current and novel approaches to downstream processing of microalgae: A review

“…The impingment stress was reported as being proportional to the operating relative pressure in some types of homogenisers (Chisti and Moo-Young, 1986). As pressure drop is one of the main cell-breaking mechanisms, the system operating pressure is critical for process performance (Balduyck et al, 2018;Halim et al, 2012;Onumaegbu et al, 2018;Samarasinghe et al, 2012). Increasing the pressure in the HPH of Chlorella sp.…”

Enhancing Stability of Microalgae Biocathode by a Partially Submerged Carbon Cloth Electrode for Bioenergy Production from Wastewater

“…Microbial fuel cell (MFC) is an attractive technology for sustainable development [1,2] that could be applied to wastewater treatment with bioelectricity generation [3,4]. Photosynthetic microalgae microbial fuel cell (MFC) could produce microbial lipid (could be converted to biodiesel [5]) and algal biomass besides electricity, while reducing greenhouse gas CO 2 by photosynthesis [6,7]. Many studies have demonstrated that the bioelectricity generation was correlated with oxygen availability in the cathode as O 2 is the electron acceptor [8][9][10].…”