Optimization of platelet‐rich fibrin

Miron, Richard J.; Fujioka‐Kobayashi, Masako; Sculean, Anton; Zhang, Yufeng

doi:10.1111/prd.12521

Cited by 20 publications

(14 citation statements)

References 37 publications

(90 reference statements)

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“…In the evolving field of regenerative medicine for periodontitis, a range of innovative therapies have demonstrated the potential to promote tissue regeneration and enhance the outcomes of conventional treatments [ 48 ]. Among these, Platelet-rich plasma (PRP) and Platelet-rich fibrin (PRF), both derived from the patient’s blood and rich in growth factors, have been shown to stimulate tissue healing and bone regeneration effectively [ 49 , 50 ]. Similarly, stem cell therapy, particularly the use of mesenchymal stem cells (MSCs) harvested from bone marrow or adipose tissue, and tissue engineering techniques involving bioactive scaffolds that deliver cells, drugs, or proteins directly to the sites of damage, have garnered attention [ 51 , 52 ].…”

Section: Discussionmentioning

confidence: 99%

Clinical Efficacy of Extracellular Vesicle Therapy in Periodontitis: Reduced Inflammation and Enhanced Regeneration

Puletic,

Velikic,

Maric

et al. 2024

IJMS

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Periodontitis, a prevalent inflammatory condition, affects the supporting structures of teeth, leading to significant oral health challenges. Traditional treatments have primarily focused on mechanical debridement, antimicrobial therapy, and surgery, which often fail to restore lost periodontal structures. Emerging as a novel approach in regenerative medicine, extracellular vesicle (EV) therapy, including exosomes, leverages nano-sized vesicles known for facilitating intercellular communication and modulating physiological and pathological processes. This study is a proof-of-concept type that evaluates the clinical efficacy of EV therapy as a non-surgical treatment for stage I–III periodontitis, focusing on its anti-inflammatory and regenerative potential. The research involved seven patients undergoing the therapy, and seven healthy individuals. Clinical parameters, including the plaque index, bleeding on probing, probing depth, and attachment level, were assessed alongside cytokine levels in the gingival crevicular fluid. The study found significant improvements in clinical parameters, and a marked reduction in pro-inflammatory cytokines post-treatment, matching the levels of healthy subjects, underscoring the therapy’s ability to not only attenuate inflammation and enhance tissue regeneration, but also highlighting its potential in restoring periodontal health. This investigation illuminates the promising role of EV therapy in periodontal treatment, advocating for a shift towards therapies that halt disease progression and promote structural and functional restoration of periodontal tissues.

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Section: Discussionmentioning

confidence: 99%

Clinical Efficacy of Extracellular Vesicle Therapy in Periodontitis: Reduced Inflammation and Enhanced Regeneration

Puletic,

Velikic,

Maric

et al. 2024

IJMS

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“…It is important to best optimize PRF as presented in a recent article on the topic. 21 After processing, it is possible to observe separation of the blood layers into plasma and the remaining decanted red cells.…”

Section: Clinical Protocol To Produce Albumin Gel Plus Platelet Rich ...mentioning

confidence: 99%

Extended platelet‐rich fibrin

Miron,

Pikos,

Estrin

et al. 2023

Periodontology 2000

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Platelet‐rich fibrin (PRF) has been characterized as a regenerative biomaterial that is fully resorbed within a typical 2–3 week period. Very recently, however, a novel heating process was shown to extend the working properties of PRP/PRF from a standard 2–3 week period toward a duration of 4–6 months. Numerous clinicians have now utilized this extended‐PRF (e‐PRF) membrane as a substitute for collagen barrier membranes in various clinical applications, such as guided tissue/bone regeneration. This review article summarizes the scientific work to date on this novel technology, including its current and future applications in periodontology, implant dentistry, orthopedics and facial aesthetics. A systematic review was conducted investigating key terms including “Bio‐Heat,” “albumin gel,” “albumin‐PRF,” “Alb‐PRF,” “extended‐PRF,” “e‐PRF,” “activated plasma albumin gel,” and “APAG” by searching databases such as MEDLINE, EMBASE and PubMed. Findings from preclinical studies demonstrate that following a simple 10‐min heating process, the transformation of the liquid plasma albumin layer into a gel‐like injectable albumin gel extends the resorption properties to at least 4 months according to ISO standard 10 993 (subcutaneous animal model). Several clinical studies have now demonstrated the use of e‐PRF membranes as a replacement for collagen membranes in GTR/GBR procedures, closing lateral windows in sinus grafting procedures, for extraction site management, and as a stable biological membrane during recession coverage procedures. Furthermore, Alb‐PRF may also be injected as a regenerative biological filler that lasts extended periods with advantages in joint injections, osteoarthritis and in the field of facial aesthetics. This article highlights the marked improvement in the stability and degradation properties of the novel Alb‐PRF/e‐PRF technology with its widespread future potential use as a potential replacement for collagen membranes with indications including extraction site management, GBR procedures, lateral sinus window closure, recession coverage among others, and further highlights its use as a biological regenerative filler for joint injections and facial aesthetics. It is hoped that this review will pioneer future opportunities and research development in the field, leading to further progression toward more natural and less costly biomaterials for use in medicine and dentistry.

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Section: Introductionmentioning

confidence: 99%

“…Platelet-rich fibrin is prepared from fractionated blood, where the plasma components are separated from the erythrocytes before coagulation [1,2]. The plasma fraction containing the platelets and leukocytes undergoes coagulation, and the resulting yellow clot is removed while serum is squeezed out, ending up with PRF membranes [1]. PRF membranes have a broad spectrum of clinical applications ranging from dentistry [3][4][5][6][7][8][9] to other disciplines, such as healing diabetic ulcers [10], cartilage and joint repair [11], and even pterygium surgery [12].…”

Section: Introductionmentioning

confidence: 99%

Human versus Rat PRF on Collagen Membranes: A Pilot Study of Mineralization in Rat Calvaria Defect Model

Apaza Alccayhuaman,

Heimel,

Tangl

et al. 2024

Bioengineering

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Platelet-rich fibrin, the coagulated plasma fraction of blood, is commonly used to support natural healing in clinical applications. The rat calvaria defect is a standardized model to study bone regeneration. It remains, however, unclear if the rat calvaria defect is appropriate to investigate the impact of human PRF (Platelet-Rich Fibrin) on bone regeneration. To this end, we soaked Bio-Gide® collagen membranes in human or rat liquid concentrated PRF before placing them onto 5 mm calvarial defects in Sprague Dawley rats. Three weeks later, histology and micro-computed tomography (μCT) were performed. We observed that the collagen membranes soaked with rat PRF show the characteristic features of new bone and areas of mineralized collagen matrix, indicated by a median mineralized volume of 1.5 mm3 (range: 0.9; 5.3 mm3). Histology revealed new bone growing underneath the membrane and hybrid bone where collagen fibers are embedded in the new bone. Moreover, areas of passive mineralization were observed. The collagen membranes soaked with human PRF, however, were devoid of histological features of new bone formation in the center of the defect; only occasionally, new bone formed at the defect margins. Human PRF (h-PRF) caused a median bone volume of 0.9 mm3 (range: 0.3–3.3 mm3), which was significantly lower than what was observed with rat PRF (r-PRF), with a BV median of 1.2 mm3 (range: 0.3–5.9 mm3). Our findings indicate that the rat calvaria defect model is suitable for assessing the effects of rat PRF on bone formation, but caution is warranted when extrapolating conclusions regarding the efficacy of human PRF.

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Optimization of platelet‐rich fibrin

Cited by 20 publications

References 37 publications

Clinical Efficacy of Extracellular Vesicle Therapy in Periodontitis: Reduced Inflammation and Enhanced Regeneration

Clinical Efficacy of Extracellular Vesicle Therapy in Periodontitis: Reduced Inflammation and Enhanced Regeneration

Extended platelet‐rich fibrin

Human versus Rat PRF on Collagen Membranes: A Pilot Study of Mineralization in Rat Calvaria Defect Model

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