Optimization of physical and nutritional parameters for hyaluronidase production by <i> Streptococcus mitis</i>

Abstract

The effect of some physical and nutritional parameters were studied for the optimum production of extracellular enzyme hyaluronidase employing Streptococcus mitis MTCC*2695 by submerged fermentation. The effects of initial pH, incubation temperature and time, inoculum level and age of inoculum were studied. The maximum enzymatic activity was obtained with an initial pH 5.8, incubation temperature 37°, incubation time for 48 h and inoculum level 6% with inoculum age 24 h. The effect of different carbon and nitrogen sources and antibiotics were studied. The results indicated that sucrose and ammonium chloride showed the highest enzymatic activity among various carbon and nitrogen sources. Antibiotic clarithromycin showed strong inhibitory effect on hyaluronidase production.

Optimization and Production of Hyaluronidase by Streptococcus mitis MTCC 2695

“…This concludes that pH 4, supports organism metabolic rates actively. The rate of product was high when compared to Sahoo et al (Figure 4) [4].…”

“…Usually a 0.2 ml of the drugs is injected around the area of extravasation. Some bacteria, such as Staphylococcus aureus, Streptococcus pyogenes and Clostridium perfringens [19,[22][23][24][25][26] produce hyaluronidase as a means for greater mobility through the body's tissues [27] and as an antigenic distinguish that prevents their being recognized by phagocytes of the immune system [11,28]. Hyaluronidase has been investigated as an additive to chemotherapeutic drugs for augmentation of the anticancer effect [29].…”

“…A pathological isolate Streptococcus equi SED 9 was used for production of an extracellular enzyme hyaluronidase by using various carbon sources. Among them dextrose enhanced production and it was found to be 271 U/ml [4,25].…”

“…The experiments were carried out for the production of various pH on hyaluronidase production by using Streptococcus equi SED 9. Among them pH 5.5 influenced the maximum yield of enzyme production and it was found to be 165 U/ml [4,24].…”

“…Hyaluronidase is naturally found in mammals, insects, leaches and bacteria [2]. Hyaluronidase can be produced by submerged fermentation from Streptococcus mitis [3][4][5]. It is a valuable enzyme because it can acts as an adjuvant, which accelerates and increases the absorption and dispersion of injected drugs [6].…”