Optimization of phycobiliprotein pigments extraction from red algae Gracilaria gracilis for substitution of synthetic food colorants

Pereira, Tatiana; Barroso, Sónia; Mendes, Susana; Amaral, Renata A.; Dias, Juliana R.; Baptista, Teresa; Saraiva, Jorge A.; Alves, Nuno; Gil, Maria M.

doi:10.1016/j.foodchem.2020.126688

Cited by 67 publications

(29 citation statements)

References 37 publications

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“…The presence of phycobiliproteins may also contribute to the overall antioxidant potential of the aqueous extract per gram of dry extract. The quantification of phycobiliproteins lay beyond the scope of this project, but has been previously quantified for wild G. gracilis , and appears less subjected to seasonal variation as the overall antioxidant activities and protein content [ 26 , 27 , 28 ].…”

Section: Resultsmentioning

confidence: 99%

Optimization of Extraction Conditions for Gracilaria gracilis Extracts and Their Antioxidative Stability as Part of Microfiber Food Coating Additives

et al. 2020

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Incorporation of antioxidant agents in edible films and packages often relies in the usage of essential oils and other concentrated hydrophobic liquids, with reliable increases in antimicrobial and antioxidant activities of the overall composite, but with less desirable synthetic sources and extraction methods. Hydroethanolic extracts of commercially-available red macroalgae Gracilaria gracilis were evaluated for their antioxidant potential and phenolic content, as part of the selection of algal biomass for the enrichment of thermoplastic film coatings. The extracts were obtained through use of solid-liquid extractions, over which yield, DPPH radical reduction capacity, total phenolic content, and FRAP activity assays were measured. Solid-to-liquid ratio, extraction time, and ethanol percentages were selected as independent variables, and response surface methodology (RSM) was then used to estimate the effect of each extraction condition on the tested bioactivities. These extracts were electrospun into polypropylene films and the antioxidant activity of these coatings was measured. Similar bioactivities were measured for both 100% ethanolic and aqueous extracts, revealing high viability in the application of both for antioxidant coating purposes, though activity losses as a result of the electrospinning process were above 60% in all cases.

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Section: Resultsmentioning

confidence: 99%

Optimization of Extraction Conditions for Gracilaria gracilis Extracts and Their Antioxidative Stability as Part of Microfiber Food Coating Additives

et al. 2020

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“…The extraction of PBP pigments from G. gracilis was done by maceration of lyophilized biomass suspended in phosphate buffer pH 6.8, using previously optimized conditions (Pereira et al., 2020). The stability of the pigment in the extracts was then studied and, after purification, the pigment was applied in two food products: pancakes and yogurts.…”

Section: Resultsmentioning

confidence: 99%

“…PE extraction was performed as described in Pereira et al. (2020). Lyophilized biomass was suspended in phosphate buffer pH 6.8 (0.1 M) at a biomass:buffer ratio of 1:50 and homogenized for 10 min with the help of a magnetic stirrer at room temperature, followed by 10 min of maceration with a mortar and pestle.…”

Section: Methodsmentioning

confidence: 99%

Stability, kinetics, and application study of phycobiliprotein pigments extracted from red algae Gracilaria gracilis

Pereira

Barroso

Mendes

et al. 2020

Journal of Food Science

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Phycobiliprotein (PBP) pigments were extracted from red algae Gracilaria gracilis through maceration in phosphate buffer using previously optimized conditions. The stability of PBPs in the extracts was assessed by monitoring the extracts at different pHs and temperatures for 10 days. Since phycoerythrin (PE) is the main PBP present in G. gracilis, PE content was spectroscopically determined and used as a response factor. Kinetic modeling was used to describe PE degradation under different ranges of T and pH. The pigment extracts presented higher stability at pH 6.9 and −20°C. PE was semipurified by precipitation with ammonium sulphate 65% followed by dialysis against water until a purity index of 0.7. The pigment was successfully applied as colorant in pancakes and yogurts with a pigment concentration of 0.15%. This study highlights the potential of PE pigments extracted from G. gracilis for applications in food products.

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“…Phycobiliproteins are water soluble protein found in Rhodophyta (red algae), Cyanobacteria (Spirulina), and Cryptophyta (Table 2). These proteins are well known for their strong antioxidant and free-radical scavenging activities [124]. Phycobiliproteins are divided in three classes phycoerythrin, phycocyanin and allophycocyanin.…”

Section: Proteins and Peptidesmentioning

confidence: 99%

“…These proteins constitute up to 60% of the total soluble cellular protein in microalgae [125]. Phycobiliproteins have high commercial value as natural colorants in the nutraceutical, cosmetic, and pharmaceutical industries [124].…”

Section: Proteins and Peptidesmentioning

confidence: 99%

Valorization of Natural Antioxidants for Nutritional and Health Applications

Ferreira-Santos¹,

Genisheva²,

Botelho³

et al. 2021

Antioxidants - Benefits, Sources, Mechanisms of Action

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The significant increase in the world population age, 47 years in 1950 to 73 years in 2020, resulted in an increase in aging related diseases as well as in degenerative diseases. In consequence, researchers have been focusing in the development of new therapies, with a particular emphasis on the use of compounds with antioxidant properties, namely phytochemicals, such as polyphenols and carotenoids. Several in vitro and in vivo studies have demonstrated the phytochemicals antioxidant capacity. Their use is broad, as they can be part of food supplements, medicine and cosmetics. The health benefit of antioxidant phytochemicals is an indisputable question. Phytochemical properties are highly influenced by the natural matrix as well as by extraction process, which have a key role. There are several extraction methods that can be applied depending on the chemical properties of the bioactive compounds. There is a wide range of solvents with different polarities, which allows a selective extraction of the desired target family of compounds. Greener technologies have the advantage to reduce extraction time and solvent quantity in comparison to the most traditional methods. This chapter will focus on the different green extraction strategies related to the recovery of antioxidant bioactive compounds from natural sources, their nutritional and health potential.

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Optimization of phycobiliprotein pigments extraction from red algae Gracilaria gracilis for substitution of synthetic food colorants

Cited by 67 publications

References 37 publications

Optimization of Extraction Conditions for Gracilaria gracilis Extracts and Their Antioxidative Stability as Part of Microfiber Food Coating Additives

Optimization of Extraction Conditions for Gracilaria gracilis Extracts and Their Antioxidative Stability as Part of Microfiber Food Coating Additives

Stability, kinetics, and application study of phycobiliprotein pigments extracted from red algae Gracilaria gracilis

Valorization of Natural Antioxidants for Nutritional and Health Applications

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