Optimization of Penicillin G Acylase Immobilization Process by Surface Response Methodology Using Central Composite Design

Abstract

<p align="justify"> <span>Penicillin G acylase was immobilized onto superparamagnetite iron oxide nanoparticles employing response surface methodology through central composite design. Polynomial quadratic model was selected as a model. The value of the determination coefficient (R²) calculated from the quadratic regression model was 0.845, while the value of the adjusted (R²) was 0.74. The regression analysis of the data showed that the quadratic model selected were appropriate thereby enzyme concentration (A), reaction temperature (D), enzyme concentration^* reaction temperature (AD), quadratics enzyme concentration (A²) and reaction temperature (D²) were found to be significant factors in immobilization process of penicillin G acylase.</span><span></span> </p

Protein recovery as a resource from waste specifically via membrane technology—from waste to wonder

“…To date, researchers have been focusing on the development of efficient adsorbents for the immobilization of protein, as there is a crucial need for the fruitful implementation of its applications in different areas. The selection of an appropriate adsorbent for the adsorption of protein depends on several factors, including surface area and large pore sizes with a volume ratio that can be placed on proteins and enzymes with ease (Han et al 2007 ; He and Shi 2011 ; Hartmann and Kostrov 2013 ; Mohammad 2013 ; Gascón et al 2014 ; Masuda et al 2014 ; Deka et al 2015 ; Tu et al 2016 ). Numerous categories of mesoporous silica substances including MCM-41 (Salis et al 2009 ), SBA-15 (Washmon-Kriel et al 2000 ), MCF and FMS (Kim et al 2005 ) have been effectively used for the immobilization of different substances, e.g.…”