Optimization of parameters for the alcoholic-assisted dispersive liquid–liquid microextraction of estrogens in water

Shakeri, Panteha; Kiasari, Zahra Mousavi; Hadjmohammadi, Mohammad Reza; Fatemi, M.

doi:10.1007/s13738-013-0403-5

Cited by 9 publications

(1 citation statement)

References 25 publications

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“…DLLME is based on the formation of ternary system consisting of the aqueous phase and mixture of two solvents . The method has been mostly employed for the isolation of estrogens from water samples [, ]; however, effective DLLME modification for urine sample and DLLME procedure for milk and yoghurt have been recently published for the extraction of estrogens, thus indicating the applicability even in the case of more complex matrices.…”

Section: Introductionmentioning

confidence: 99%

Dispersive liquid–liquid microextraction as an effective preanalytical step for the determination of estradiol in human urine

Kupcová

Reiffová

2017

J of Separation Science

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In this work, a fast and effective dispersive liquid-liquid microextraction was developed for the isolation and preconcentration of free 17 β-estradiol, the main human estrogen, from real human urine samples. To optimize the extraction technique, few important parameters such as type and volume of extraction and dispersive solvents, centrifugation conditions, effect of salt addition, and extraction time were studied. Optimal conditions were obtained when injecting 600 μL mixture of tetrachloromethane as extraction solvent and ethanol as dispersive solvent (1:5, v/v) into 2 mL of urine containing 8% NaCl and following centrifugation at 10 000 rpm, thus reaching enrichment factor 28 and extraction recovery 98% for estradiol. Procedure was evaluated by means of high-performance liquid chromatography with UV detection (λ = 280 nm) using a C-18 column and methanol/water (60:40, v/v) as the mobile phase. The method was linear within the concentration range 1.0-250.0 mg/L (r = 0.9997) and provided a limit of detection of 0.25 mg/L. The proposed method was applied to the determination of free estradiol in real human pregnancy urine.

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Section: Introductionmentioning

confidence: 99%

Dispersive liquid–liquid microextraction as an effective preanalytical step for the determination of estradiol in human urine

Kupcová

Reiffová

2017

J of Separation Science

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Ultrasound-assisted dispersive liquid–liquid microextraction method combined with HPLC-fluorescence detection for the determination of glycyrrhetinic acid in liquorice and liquorice-derived food products

You

et al. 2015

J IRAN CHEM SOC

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Utilization of water-contained surfactant-based ultrasound-assisted microextraction followed by liquid chromatography for determination of polycyclic aromatic hydrocarbons and benzene in commercial oil sample

Amlashi

Hadjmohammadi

2016

J IRAN CHEM SOC

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Optimization of parameters for the alcoholic-assisted dispersive liquid–liquid microextraction of estrogens in water

Cited by 9 publications

References 25 publications

Dispersive liquid–liquid microextraction as an effective preanalytical step for the determination of estradiol in human urine

Dispersive liquid–liquid microextraction as an effective preanalytical step for the determination of estradiol in human urine

Ultrasound-assisted dispersive liquid–liquid microextraction method combined with HPLC-fluorescence detection for the determination of glycyrrhetinic acid in liquorice and liquorice-derived food products

Utilization of water-contained surfactant-based ultrasound-assisted microextraction followed by liquid chromatography for determination of polycyclic aromatic hydrocarbons and benzene in commercial oil sample

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