Optimization of Parameters for a More Efficient Use of Adipose-Derived Stem Cells in Regenerative Medicine Therapies

Abstract

<jats:p>Adipose tissue-derived stem cells (ASCs) association to fat in autologous lipotransfer is promising for a more effective soft tissue reconstruction, and optimization of protocols to isolate ASCs from lipoaspirate fat is much needed. We demonstrated that an increase in adipocyte differentiation is dependent on the number of ASCs. In a sample of 10 donors, we found a higher concentration of nucleated cells in the lower abdomen compared to flank (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mi>P</mml:mi><mml:mo>=</mml:mo><mml:mn>0.015</mml:mn></mml:math>). In a sample of 6 donors we did not find differences in the cell yield obtained by manual or pump-assisted aspiration (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mi>P</mml:mi><mml:mo>=</mml:mo><mml:mn>0.56</mml:mn></mml:math>). We suggest that the increase in the number of ASCs in the reinjected fat may enhance the efficiency of newly formed adipose tissue and that the anatomical region from which to harvest fat tissue needs to be considered to optimize the number of ASCs in the harvested tissue. Finally, pump-assisted aspiration can be used without any significant harm to the viability of cells.</jats:p>

Harvest site influences the growth properties of adipose derived stem cells

“…ASCs were harvested as described previously by our group (di Summa et al 2010). One gram of adipose tissue was harvested from the most representative sources according to the literature (Aguena et al 2012;Bayes-Genis et al 2012;Mohammadi et al 2012;Padoin et al 2008;Yuan et al 2010) (inguinal, flank, pericardiac, neck and omentum) in four male SpragueDawley (SD) rats aged 8 weeks weighing approximately 250 g. SD rats were selected because they are widely used in the field of tissue engineering and peripheral nerve repair (di Summa et al 2010). The adipose tissue was carefully dissected, minced by using a sterile razor blade and placed for 2 h at 37°C in 15 ml Hank's balanced salt solution (HBSS) containing 0.2 % (w/v) collagenase type 1 and 20 ll penicillin/streptomycin for enzymatic digestion.…”

Fat Grafts Supplemented with Adipose-Derived Stromal Cells in the Rehabilitation of Patients with Craniofacial Microsomia

“…Results classification based on percentage of fat graft survival regarding grafted volume showed unsatisfactory results for 57.1 percent of the control group and a predominance of excellent results for the experimental group (experimental (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31) group: excellent results, 7; control group: excellent, 1; good, 2; unsatisfactory, 4). Through the calculation of relative risk and its variables, it was observed that the experimental group would be seven times as likely as the control group to obtain an excellent result, and an excellent result was attributable to the intervention of supplementation of adipose-derived stromal cells in 85 percent (p = 0.005) ( Table 7).…”

Adipose Tissue-Derived Mesenchymal Stem Cells: A Novel Therapy in Orthopedics

“…The ASCs undergo several rounds of centrifugation and dilution through which collagenase is washed off and the remaining product is the stromal vascular fraction (SVF) [35,[50][51][52][53][54]. SVF has been used as a source of cells useful for tissue regeneration.…”