“…Alternatively, researchers may use our vectors that already contain the engineered RNA leaders (available through Addgene). To date, our lab has utilized our RNA leader sequences to tune the expression levels of GFP, mCherry, mTagBFP, mKate, c-Myc, p53, p21, H-Ras, 20 FKBP, and DHFR. For applications where the protein of interest can be toxic or applications where conditional or dynamic control of expression is desired, we have found it desirable to specify the magnitude of expression by controlling translation and to conditionally turn on expression using small-molecule inducible control of protein localization (estrogen-receptor fusion with nuclear localization induced by addition of 4-hydroxytamoxifen) and stability (DHFR-fusion stabilized by trimethoprim, Fig.…”