Optimization of nutritional and environmental conditions for biomass production and sporulation of entomogenous fungus Lecanicillium lecanii CA-1-G using two-stage cultivation system

Gao, Li

doi:10.5897/jyfr2017.0180

J. Yeast Fungal Res.

2018

DOI: 10.5897/jyfr2017.0180

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Optimization of nutritional and environmental conditions for biomass production and sporulation of entomogenous fungus Lecanicillium lecanii CA-1-G using two-stage cultivation system

Li Gao¹

Abstract: for another 4 days with the environmental conditions of-7.3 MPa/pH 4/light 24 h/temperature 29°C for sporulation and-7.3 MPa/pH 3/light 12h/temperature 23°C for mass production. This research reflects on some physiology on nutrients and environmental characteristics of this fungus, and also gives the essential information for its industrialization.

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Cited by 2 publications

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Morphological and molecular identification of four isolates of the entomopathogenic fungal genus Akanthomyces and their effects against Bemisia tabaci on cucumber

Broumandnia

Rajabpour

Parizipour

et al. 2021

Bull. Entomol. Res.

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The cotton whitefly, Bemisia tabaci Gen. (Hem., Aleyrodidae), is a key pest of many vegetables. Entomopathogenic fungi are promising microbial control agents against B. tabaci, but limited information is available concerning indigenous Iranian isolates. In this study, three isolates of Akanthomyces lecanii (PAL6, PAL7, and PAL8) and one isolate of A. muscarius (AGM5) were obtained from citrus hemipteran pests, Pulvinaria aurantii Cock. and Aphis gossypii Glover, in Mazandaran province, northern Iran. The isolates were then morphologically and molecularly identified. The efficacies of five different agar media for vegetative growth and conidiation of each isolate were determined. Potato dextrose agar was the medium on which the fungal mycelia developed at a relatively high rate. However, the highest rate of conidiation was found on Sabouraud dextrose agar. To determine the effects of the isolates on B. tabaci, a dose–response bioassay was carried out to estimate lethal concentration (LC50) and lethal time (LT50) values of each fungal isolate to second instar nymphs. The mean LC50 values of A. lecanii isolates ranged from 4.22 × 106 to 7.35 × 1013 conidia ml−1 at 5 to 7 days after the treatment. For A. muscarius, the values varied from 9.2 × 104 to 8.7 × 1010 conidia ml−1 at 5 to 7 days after the treatment. The lowest and the highest mean LC50 values were observed for A. mucarius (AGM5) and A. lecanii (isolate PAL6), respectively. The mean LT50 values of A. lecanii and A. muscarius isolates were 7.1–9.0 and 4.9–7.2 days, respectively. The LT50 values of A. muscarius were significantly lower than the other isolates. Overall, all isolates, especially A. muscarius (AGM5), exhibited appropriate potential as a biological control agent against B. tabaci.

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Morphological and molecular identification of four isolates of the entomopathogenic fungal genus Akanthomyces and their effects against Bemisia tabaci on cucumber

Broumandnia

Rajabpour

Parizipour

et al. 2021

Bull. Entomol. Res.

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A New Strain of Lecanicillium uredinophilum Isolated from Tibetan Plateau and Its Insecticidal Activity

2022

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A new strain QHLA of Lecanicillium uredinophilum was isolated from a Chinese caterpillar fungus complex and its optimum growth temperature and fermentation conditions were studied. Its insecticidal activity was tested against larvae of seven different insect pests, including Henosepilachna vigintioctopunctata, Spodoptera exigua, Plutella xylostella, Spodoptera frugiperda, Sitobion avenae, Hyalopterus perikonus, and Aphis citricola. The optimum growth temperature was 21–24 °C. The highest spore production of the strain QHLA was 4.08 × 106 spore/mL on solid medium with a nitrogen source of NH4Cl. However, the highest mycelial growth rate of the strain QHLA was on solid medium with a nitrogen source from yeast extract (4.63 ± 0.03 mm/d). When the liquid medium contained peptone, yeast extract, and glucose, the water content of the mycelia was the lowest, while the spore production was the highest until day 12. When the liquid medium contained glucose, tussah pupa powder, KH2PO4, and MgSO4, the mycelia production was highest until day 8. The bioassay for insecticidal activity indicated that the LC50 values of QHLA were 6.32 × 103 spore/mL and 6.35 × 103 spore/mL against Sitobion avenae and Aphis citricola, respectively, while the LC90 values of the strain QHLA against Aphis citricola and Sitobion avenae were 2.11 × 107 spore/mL and 2.36 × 108 spore/mL, respectively. Our results demonstrated that the strain QHLA was a high virulence pathogenic fungus against insect pests, with the potential to be developed as a microbial pesticide.

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Optimization of nutritional and environmental conditions for biomass production and sporulation of entomogenous fungus Lecanicillium lecanii CA-1-G using two-stage cultivation system

Cited by 2 publications

References 7 publications

Morphological and molecular identification of four isolates of the entomopathogenic fungal genus Akanthomyces and their effects against Bemisia tabaci on cucumber

Morphological and molecular identification of four isolates of the entomopathogenic fungal genus Akanthomyces and their effects against Bemisia tabaci on cucumber

A New Strain of Lecanicillium uredinophilum Isolated from Tibetan Plateau and Its Insecticidal Activity

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