Optimization of niosomes for enhanced antibacterial activity and reduced bacterial resistance:in vitroandin vivoevaluation

Abdelaziz, Ahmed; El-Banna, Tarek; Sonbol, Fatma Ibrahim; Gamaleldin, Noha M.; Maghraby, Gamal M. El

doi:10.1517/17425247.2014.942639

Cited by 62 publications

(37 citation statements)

References 65 publications

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“…The increased absorptive clearance was associated with a reduction in the L95% both in the duodenum and jejuno‐illeum segments (Table ). These data can be explained on the bases that niosomes are capable of carrying the nonionized drug in the lipid membrane with the ionized form being located in the aqueous compartment of the vesicle . Enhanced bioavailability was previously reported after oral administration of lipophilic drugs in niosomes .…”

Section: Resultssupporting

confidence: 56%

“…The polydispersity index was 0.5 (SD = 0.03) reflecting the heterogeneity of the vesicles which is expected with those prepared by bath sonication. These results correlate with the published data which were generated for vesicles prepared by the same technique . With respect to the SMEDDS, the system resulting after its dilution was in the nanorange with an average diameter of 81 (SD = 2) which confirms the constructed phase diagram.…”

Section: Resultsmentioning

confidence: 99%

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Colloidal carriers for extended absorption window of furosemide

Sultan

El‐Gizawy

Osman

et al. 2016

Journal of Pharmacy and Pharmacology

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Section: Resultssupporting

confidence: 56%

Section: Resultsmentioning

confidence: 99%

Colloidal carriers for extended absorption window of furosemide

Sultan

El‐Gizawy

Osman

et al. 2016

Journal of Pharmacy and Pharmacology

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“…These proposed mechanisms of enhancement were confirmed by the lack of any antimicrobial activity of blank niosomes, which was in agreement with the findings of other studies. 15,16 The difference in the antimicrobial effect, between the two niosomal preparations or the difference from that reported in Satish et al's study, may have resulted from the effect of the surfactant used. Span 60 (used in preparation II) has a longer saturated alkyl chain than that of span 40, where the length of alkyl chain of used surfactants is an important factor affecting the permeability of the prepared niosomes.…”

Section: Discussionmentioning

confidence: 87%

“…12,14 The effect of niosomes in the enhancement of vancomycin and norfloxacin antibiofilm activity, against S. aureus and P. aeruginosa, was reported previously. 15,16 This study aimed at preparing ciprofloxacin-loaded niosomes and testing their potential antimicrobial effect against ciprofloxacin-resistant S. aureus clinical isolates as well as the possible inhibition of biofilm formation or eradication of already formed biofilms. Two different formulations of ciprofloxacin-loaded niosomes were prepared.…”

Section: Introductionmentioning

confidence: 99%

The Antimicrobial Activity of Ciprofloxacin-Loaded Niosomes against Ciprofloxacin-Resistant and Biofilm-Forming Staphylococcus aureus

Kashef

Saleh

Assar

et al. 2020

IDR

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The threat of Staphylococcus aureus antimicrobial resistance is increasing worldwide. Niosomes are a new drug delivery system that enhances the antimicrobial potential of antibiotics. We hereby aim to evaluate the antimicrobial and antibiofilm activity of ciprofloxacin-loaded niosomes. Methods: The antimicrobial susceptibility of clinical S. aureus isolates (n=59) was determined by Kirby-Bauer disk diffusion method. Their biofilm formation activity was tested by Christensen's method. Two ciprofloxacin-loaded niosomal formulations were prepared by thin-film hydration method, and their minimum inhibitory concentrations (MIC) were determined by agar dilution method, against ciprofloxacin-resistant and biofilm-forming isolates (n=24). Their ability to inhibit biofilm formation and eradicate already formed biofilms was evaluated and further confirmed by scanning electron microscope images. Non-synonymous mutations, in a quinolone resistance-determining regions of S. aureus isolates, were detected by polymerase chain reaction. Results: Most of the isolates were methicillin-(47/59) and ciprofloxacin-resistant (45/59). All except two isolates were capable of biofilm production. Niosomal preparation I reduced ciprofloxacin MIC by twofold in four isolates, whereas preparation II reduced ciprofloxacin MIC of most isolates by 8-to 32-fold, with three isolates that became ciprofloxacinsusceptible. Non-synonymous mutations were detected in isolates that maintained phenotypic ciprofloxacin resistance against ciprofloxacin-loaded niosomal preparation II. Ciprofloxacinloaded niosomes reduced the minimum biofilm inhibitory concentration and the minimum biofilm eradication concentration in 58% and 62% of the tested isolates, respectively. Conclusion: Ciprofloxacin-loaded niosomes can restore ciprofloxacin activity against resistant S. aureus isolates. To our knowledge, this is the first report on the inhibition of biofilm formation and eradication of formed biofilms by ciprofloxacin-loaded niosomes.

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“…Changes in bacterial diversity, microbiota composition, and host-defence interactions in health and disease can be studied using skin models. For instance, human reconstructed skin models and ex vivo skin models have been used to study the efficacy of antibacterial compounds (21)(22)(23)(24) or the colonization and infection characteristics of certain pathogens (25)(26)(27)(28)(29). However, these models are laborious, expensive, prone to bacterial overgrowth, and their throughput is low.…”

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confidence: 99%

An In vitro Model for Bacterial Growth on Human Stratum Corneum

Krieken¹,

Ederveen²,

Hijum³

et al. 2016

Acta Derm Venerol

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The diversity and dynamics of the skin microbiome in health and disease have been studied recently, but adequate model systems to study skin microbiotas in vitro are largely lacking. We developed an in vitro system that mimics human stratum corneum, using human callus as substrate and nutrient source for bacterial growth. The growth of several commensal and pathogenic bacterial strains was measured for up to one week by counting colony-forming units or by quantitative PCR with strain-specific primers. Human skin pathogens were found to survive amidst a minimal microbiome consisting of 2 major skin commensals: Staphylococcus epidermidis and Propionibacterium acnes. In addition, complete microbiomes, taken from the backs of healthy volunteers, were inoculated and maintained using this system. This model may enable the modulation of skin microbiomes in vitro and allow testing of pathogens, biological agents and antibiotics in a medium-throughput format.

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Optimization of niosomes for enhanced antibacterial activity and reduced bacterial resistance:in vitroandin vivoevaluation

Abstract: Niosomes are promising for enhanced antibacterial activity and reduced resistance to antibiotics. The later can be achieved by inhibition of biofilm formation.

Cited by 62 publications

References 65 publications

Colloidal carriers for extended absorption window of furosemide

Colloidal carriers for extended absorption window of furosemide

<p>The Antimicrobial Activity of Ciprofloxacin-Loaded Niosomes against Ciprofloxacin-Resistant and Biofilm-Forming <em>Staphylococcus aureus</em></p>

An In vitro Model for Bacterial Growth on Human Stratum Corneum

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