Optimization of Newcastle disease virus production in T-flask

Abstract

In this work, the propagation of Asplin F strain of Newcastle disease virus (NDV) in DF-1 cells was investigated. Experiments were carried out in T-flask to investigate the effects of serum concentration in the culture medium during virus replication phase and multiplicity of infection (MOI) on virus production. Virus infectivity titre of 6.62 x 10 7 TCID 50 /ml was achieved when serum concentration of 0.5% and MOI of 20 was used.

Development, characterization and optimization of a new suspension chicken-induced pluripotent cell line for the production of Newcastle disease vaccine

“…MDCK and Vero cell lines have been extensively studied and used by manufacturers for the production of approved influenza virus vaccine [12][13][14]. Unlike influenza virus vaccine production, limited avian cell lines have been explored for NDV vaccine production [15,16] hence manufacturing of NDV vaccine largely depends on embryonated eggs for production. Some primary and continuous cell lines of avian and non-avian origin have been used in the propagation of NDV.…”

“…Some primary and continuous cell lines of avian and non-avian origin have been used in the propagation of NDV. These include chick embryo fibroblast (CEF), chicken embryo liver (CEL) cells, chicken embryo kidney (CEK) cells, African green monkey kidney (Vero) cells, and DF-1 (a cell line derived from CEF) cells [16,17]. Primary cells have the disadvantage of senescence leading to the need for regular and costly re-derivation and characterization and the fact that they may not always comply with modern quality standards required for good manufacturing practices.…”

Seroprevalence and Associated Risk Factors of Newcastle Disease in Backyard Chicken Production System in Selected Districts of Ilubabor Zone, Southwestern Ethiopia

“…The disease probably emerged more than 150 years ago in wild birds and was not recognized as a poultry disease until it was first observed in 1926 in the Indonesia island of Java as chicken disease and it became a severe global problem affecting poultry production [3]. Sources of infection for NDV are exhaled air from infected birds and contaminated feed and water, Feces, eggs during clinical diseases, and all parts of the carcass during acute infection and at death can also act as sources of infection, and transmission is mostly via aerosol.…”