Optimization of Mobile Phase Composition in Liquid Chromatography—A Survey of Most Commonly Used Chemometric Procedures

Goewie, C.E.

doi:10.1080/01483918608076695

Cited by 31 publications

(5 citation statements)

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“…Having selected the separation method to be used the separation itself must be optimized to meet the goals of the analysis [478,480,481,[485][486][487][488][489][490]. Based on the number of components and their polarity range either an isocratic (section 4.6.2) or gradient elution (section 4.6.3) separation may be employed.…”

Section: Methods Development Strategies For Liquid Chromatographymentioning

confidence: 99%

The Column in Liquid Chromatography

Poole

1991

Chromatography Today

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Section: Methods Development Strategies For Liquid Chromatographymentioning

confidence: 99%

The Column in Liquid Chromatography

Poole

1991

Chromatography Today

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“…However, a recent review (8) noted that most mobile phase optimization procedures are limited to three or less components and most methodologies do not account for components that have constraints on the upper limits of their proportions.…”

Section: Introductionmentioning

confidence: 98%

Optimization of Solvent Composition for High Performance Thin-Layer Chromatography

Bayne

1987

J. of Liquid Chromatography & Related Technologies

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The methodology of mixture experiments has been applied to optimize the multicomponent solvent composition for high performance thin-layer chromatography (HPTLC). In this study, a mixture of five solvents for the mobile phase was maximized for the separation of twelve structurally related B(a)P metabolites on normal phase HPTLC plates. Volume fractions (rather than absolute quantities) of the mobile phase solvents were constrained by upper bounds by considering solvent strength. These restrictions defined an experimental region that is an irregular polyhedron formed from a truncated 4-dimensional simplex.The optimization of the expected separation response over the experimental region was based on a second-order Scheffk polynomial estimated from twenty-five experimental runs. Six functions for measuring separation based on R, values were evaluated. A new response function is proposed that overcomes the difficulties of previously published criteria.

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“…Optimization continues to be of major concern to chromatographers. One may find a large variety of strategies for optimization in the literature (12)(13)(14)(15)(16)(17)(18)(19). There are graphical approaches, such as the window-diagram method (13) and overlapping resolution mapping (18), as well as mathematical approaches (15).…”

mentioning

confidence: 99%

“…One may find a large variety of strategies for optimization in the literature (12)(13)(14)(15)(16)(17)(18)(19). There are graphical approaches, such as the window-diagram method (13) and overlapping resolution mapping (18), as well as mathematical approaches (15). Many of the existing optimization strategies require a large capacity factor data base (developed from several chromatographic runs) and are thus very time-consuming (16).…”

mentioning

confidence: 99%