Optimization of metacercarial production for three digenean species by the use of petri dishes for raising lettuce-fed Galba truncatula

Rondelaud, D.; Fousi, M; Vignoles, Philippe; Moncef, Mohammed; Dreyfuss, G.

doi:10.1007/s00436-006-0353-2

Cited by 37 publications

(53 citation statements)

References 11 publications

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“…Snails from the first group were each subjected to singlemiracidium exposures, while the G. truncatula from the other four groups were individually exposed to two, three, four, or five miracidia, respectively. After exposure, snails were subsequently reared for 70 days according to the method described by Rondelaud et al (2007). In each Petri dish (diameter, 14 cm), ten G. truncatula, 150 ml of spring water, several decayed leaves of grass, one or two fragments of live water moss (Fontinalis sp.…”

Section: Lab-infected Snailsmentioning

confidence: 99%

Paramphistomum daubneyi: the number of sporocysts developing in experimentally and naturally infected Galba truncatula

2008

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Experimental infections of Galba truncatula with Paramphistomum daubneyi were carried out to determine at day 50 (at 24 degrees C) the numbers of sporocysts, which grew in infected snails via the count of first- and second-generation rediae. In snails individually exposed to one, two, three, four, or five miracidia, the numbers of first-generation rediae increased from the one-miracidium group to the five-miracidium snails (from a mean of 6.7 to 26.1), while second-generation rediae decreased in number (from 6.2 to 0.9, respectively). This scale of redial numbers was used to determine the number of sporocysts, which grew in naturally infected snails collected from sedimentary or acid soils between 1993 and 2006. In cercariae-containing snails, natural infections resulting from the development of one to five sporocysts were found in both samples of G. truncatula examined. The numbers of 3-, 4-, and 5-sporocyst infections were increasing over time since 1997, 2000, and 2003, respectively. The utility of such multiple-sporocyst infections is open to question, as the differentiation of second-generation rediae and that of procercariae were delayed and always limited. They might be interpreted as a consequence of a zoonosis, which has been spreading since 1990 in ruminants of central France.

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Section: Lab-infected Snailsmentioning

confidence: 99%

Paramphistomum daubneyi: the number of sporocysts developing in experimentally and naturally infected Galba truncatula

2008

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“…Each box contained small stones and a 2-cm-deep layer of spring water (dissolved calcium content, 60 mg/l), which was constantly aerated. In contrast, the method by Rondelaud et al (2007) was used in experiments performed in 2006. Snails were reared in 14-cm Petri dishes with ten G. truncatula and 150 ml of spring water per dish.…”

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Fasciola hepatica: the infectivity of cattle-origin miracidia had increased over the past years in central France

2007

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A retrospective study on experimental infections of Galba truncatula, originating from four populations, was carried out to determine the infectivity of Fasciola hepatica miracidia in snails either subjected to single-miracidium or bimiracidial exposures in 2006 and to compare it with results found before 1990 in other experiments using the same protocol. In single-miracidium infections, the prevalence of snail infection was significantly greater in 2006 than in experiments performed before 1980, while snail survival at day 30 postexposure showed insignificant differences. In bimiracidial infections, snail survival in two snail groups was significantly lower in 2006 than in experiments performed from 1981 to 1990, while insignificant differences were found for the other two populations of G. truncatula. Compared to results found between 1981 and 1990, the prevalence of snail infection did not significantly differ in 2006. In each population considered alone, the mean number of cercariae shed by infected snails did not significantly vary, whatever the date of experiment and the number of miracidia used for each exposure. The greater infectivity of F. hepatica miracidia towards snails might be the consequence of specific molecules such as triclabendazole used since 1990 to treat fasciolosis in cattle from central France.

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“…In the two groups with P. daubneyi, the number of snails at exposure was lower, as infected snails without shedding were more numerous for this digenean than for F. hepatica. After exposure, snails were subsequently reared for 30 days in 14-cm Petri dishes according to the method described by Rondelaud et al (2007). These dishes were placed in an air-conditioned room (24 ± 1°C) with a diurnal photoperiod of 12 hours and a 3,000-4,000 lx light intensity.…”

Section: Methodsmentioning

confidence: 99%

The redial and cercarial production of a Digenean in the snail host is lower when no cercarial shedding occurs

Dreyfuss¹,

Vignoles²,

Rondelaud³

2009

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Summary :Single-and double-miracidium exposures of Galba truncatula with Fasciola hepatica (two groups) or with Paramphistomum daubneyi (two groups) were carried out under laboratory conditions to compare parasite production in cercaria-shedding snails (CS snails) with that found in snails without emission (NCS snails). Free rediae and cercariae were thus counted in snails from both categories after their dissection at regular intervals (at 24°C). In the four groups, the numbers of free rediae and free cercariae found at day 75 post-exposure (F. hepatica) or at day 85 (P. daubneyi) were significantly greater in CS snails than in NCS ones. The number of cercariae in NCS subgroups did not show any significant variation from day 45 p.e. to day 75 (F. hepatica, the two groups) or from day 55 to day 85 (P. daubneyi, singlemiracidium infections), while it significantly decreased with increasing time of infection in the double-miracidium infections with P. daubneyi. In NCS snails, the presence of too numerous free cercariae within the snail's body (the volume of the body allows development only of a given number of rediae) might rapidly block out redial development and intraredial differentiation of other cercariae. The numerical diminution of P. daubneyi cercariae in the NCS snails (double-miracidium group) might probably be due to the lysis of new cercariae just formed, probably in reason of a lack of nutrients available for these larvae within the snail.

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Optimization of metacercarial production for three digenean species by the use of petri dishes for raising lettuce-fed Galba truncatula

Cited by 37 publications

References 11 publications

Paramphistomum daubneyi: the number of sporocysts developing in experimentally and naturally infected Galba truncatula

Paramphistomum daubneyi: the number of sporocysts developing in experimentally and naturally infected Galba truncatula

Fasciola hepatica: the infectivity of cattle-origin miracidia had increased over the past years in central France

The redial and cercarial production of a Digenean in the snail host is lower when no cercarial shedding occurs

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