Optimization of medium for enhancement of ε-Poly-L-Lysine production by Streptomyces sp. M-Z18 with glycerol as carbon source

Chen, Xusheng; Tang, Lei; Shu, Li; Liao, Lijuan; Zhang, Jianhua; Mao, Zhangming

doi:10.1016/j.biortech.2010.08.071

Cited by 54 publications

(26 citation statements)

References 17 publications

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“…PL6-3 [23] and even by this strain itself (Streptomyces sp. M-Z18) [18], the decrease of pH is slower and final pH is higher in the e-PL fermentation from glycerol by Streptomyces sp. M-Z18.…”

Section: Resultsmentioning

confidence: 98%

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Production of ε-poly-l-lysine using a novel two-stage pH control strategy by Streptomyces sp. M-Z18 from glycerol

Chen

Shu

Liao

et al. 2011

Bioprocess Biosyst Eng

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The production of ε-poly-L: -lysine (ε-PL) by Streptomyces sp. M-Z18 from glycerol was investigated in a 5-L jar-fermenter. Batch fermentations by Streptomyces sp. M-Z18 at various pH values ranging from 3.5 to 4.5 were studied. Based on the analysis of the time course of specific cell growth rate and specific ε-PL formation rate, a novel two-stage pH control strategy was developed to improve ε-PL production by shifting the culture pH from 3.5 to 3.8 after 36 h of cultivation. By applying the strategy, the maximal ε-PL concentration and productivity had a significant improvement and reached 9.13 g L(-1) and 4.76 g L(-1) day(-1), respectively, compared with those in one-stage pH control process where the pH value is controlled at 3.5 (7.83 g L(-1) and 3.13 g L(-1) day(-1)). Fed-batch fermentation with two-stage pH control strategy was also applied to produce ε-PL; final ε-PL concentration of 30.11 g L(-1) was obtained, being 3.3-fold greater than that of batch fermentation. To our knowledge, it is the first report on production of ε-PL from glycerol in fermenter scale and achievement of high ε-PL production with two-stage pH control strategy.

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Section: Resultsmentioning

confidence: 98%

“…346 [16], Streptomyces noursei NRRL 5126 [17] and Streptomyces sp. M-Z18 [18] were able to ferment glycerol. However, in all these processes, e-PL production was in minor quantity.…”

mentioning

confidence: 99%

Production of ε-poly-l-lysine using a novel two-stage pH control strategy by Streptomyces sp. M-Z18 from glycerol

Chen

Shu

Liao

et al. 2011

Bioprocess Biosyst Eng

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“…6A6, with glycerol as the major carbon source resulting yield of ∼0.76 g/ L . Similar approaches for glycerol utilization and metabolic engineering have been employed for the enhancement of clavulanic acid (Kun et al 2013;Jnawali et al 2010;Li and Townsend 2006), teicoplanin (Park et al 2007), and ε-poly-L-lysine (Chen et al 2011). Glycerol and ProFlo can be utilized as effective sources for increasing the titer of major precursors for polyketide biogenesis (Fig.…”

Section: Illustration Of Biosynthetic Mechanism Of Herboxidiene In Comentioning

confidence: 96%

Herboxidiene biosynthesis, production, and structural modifications: prospect for hybrids with related polyketide

Pokhrel

Dhakal

Jha

et al. 2015

Appl Microbiol Biotechnol

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Herboxidiene is a polyketide with a diverse range of activities, including herbicidal, anti-cholesterol, and pre-mRNA splicing inhibitory effects. Thus, production of the compound on the industrial scale is in high demand, and various rational metabolic engineering approaches have been employed to enhance the yield. Directing the precursors and cofactors pool toward the production of polyketide compounds provides a rationale for developing a good host for polyketide production. Due to multiple promising biological activities, the production of a number of herboxidiene derivatives has been attempted in recent years in a search for the key to improve its potency and to introduce new activities. Structural diversification through combinatorial biosynthesis was attempted, utilizing the heterologous expression of substrate-flexible glucosyltransferase (GT) and cytochrome P450 in Streptomyces chromofuscus to generate structurally and functionally diverse derivatives of herboxidiene. The successful attempt confirmed that the strain was amenable to heterologous expression of foreign polyketide synthase (PKS) or post-PKS modification genes, providing the foundation for generating novel or hybrid polyketides.

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“…Glycerol has also been used as a source of carbon for the production of the polymer of L-lysine -Poly-L-lysine with Streptomyces sp. (Chen et al, 2011b, Chen et al, 2011a. -Poly-L-lysine is an antimicrobial agent against bacteria, yeasts, and viruses (Shima et al, 1984) and therefore interesting for the pharmaceutical industry (Shih et al, 2004) and it is used as food preservative.…”

Section: Amino Acidsmentioning

confidence: 99%

Use of Glycerol in Biotechnological Applications

Wendisch¹,

Lindner²,

Meiswinkel³

2011

Biodiesel- Quality, Emissions and by-Products

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Optimization of medium for enhancement of ε-Poly-L-Lysine production by Streptomyces sp. M-Z18 with glycerol as carbon source

Cited by 54 publications

References 17 publications

Production of ε-poly-l-lysine using a novel two-stage pH control strategy by Streptomyces sp. M-Z18 from glycerol

Production of ε-poly-l-lysine using a novel two-stage pH control strategy by Streptomyces sp. M-Z18 from glycerol

Herboxidiene biosynthesis, production, and structural modifications: prospect for hybrids with related polyketide

Use of Glycerol in Biotechnological Applications

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