Optimization of medium composition to increase the expression of recombinant human interferon-β using the Plackett–Burman and central composite design in E. coli SE1

Pal, Dharam; Patel, Gopal; Dobariya, Prakashkumar; Nile, Shivraj Hariram; Pande, Abhay H.; Banerjee, Uttam Chand

doi:10.1007/s13205-021-02772-1

Cited by 8 publications

(5 citation statements)

References 36 publications

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“…The CCD is a response surface methodology widely used for the fermentation optimization of various products, including food, beverages, and pharmaceuticals [ 34 ]. It describes the effects of interactions between parameters in linear and quadratic models.…”

Section: Discussionmentioning

confidence: 99%

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Enhancing docosahexaenoic acid production of Schizochytrium sp. by optimizing fermentation using central composite design

Ding

Zhu

et al. 2022

BMC Biotechnol

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Docosahexaenoic acid (DHA) can improve human and animal health, particularly including anti-inflammatory, antioxidant, anticancer, neurological, and visual functions. Schizochytrium sp. is a marine heterotrophic protist producing oil with high DHA content, which is widely used in animal and food production. However, different fermentation conditions have intensive impacts on the growth and DHA content of Schizochytrium sp. Thus, this study aimed to enhance the DHA yield and concentration of Schizochytrium sp. I-F-9 by optimizing the fermentation medium. First, a single-factor design was conducted to select a target carbon and nitrogen source from several generic sources (glucose, sucrose, glycerol, maltose, corn syrup, yeast extract, urea, peptone, and ammonium sulfate). The Plackett–Burman design and the central composite design (CCD) were utilized to optimize the fermentation mediums. Schizochytrium sp. in 50-mL fermentation broth was cultured in a 250 mL shake flask at 28 °C and 200 rpm for 120 h before collecting the cell pellet. Subsequently, the cell walls were destroyed with hydrochloric acid to extract the fatty acid using n-hexane. The DHA content was detected by gas chromatography. The single-factor test indicated that glucose and peptone, respectively, significantly improved the DHA content of Schizochytrium sp. compared to the other carbon and nitrogen sources. Glucose, sodium glutamate, and sea crystal were the key factors affecting DHA production in the Plackett–Burman test (P = 0.0247). The CCD result showed that DHA production was elevated by 34.73% compared with the initial yield (from 6.18 ± 0.063 to 8.33 ± 0.052 g/L). Therefore, the results of this study demonstrated an efficient strategy to increase the yield and content of DHA of Schizochytrium sp.

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Section: Discussionmentioning

confidence: 99%

“…Response surface design has been widely utilized to optimize many fermentation process parameters, including medium composition [ 34 ]. Most variations in response surface optimization can be interpreted by the regression equation [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

Enhancing docosahexaenoic acid production of Schizochytrium sp. by optimizing fermentation using central composite design

Ding

Zhu

et al. 2022

BMC Biotechnol

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“…In a multivariable scheme, statistical experimental designs are the most effective strategies for quickly identifying significant variables, and for reducing errors in assessing the impact of diverse factors [8,33,34]. We evaluated the influence of starch nitrate medium on the synthesis of antimicrobial agents from S. qinglanensis H4 against S. aureus ATCC6538 by using Plackett-Burman design.…”

Section: Discussionmentioning

confidence: 99%

Bioactivity of Metabolites from Actinomycetes Isolates from Red Sea, Egypt

Osman

El-Nasr

Hussein

et al. 2022

Microbiol. Biotechnol. Lett.

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Actinomycetes isolated from marine habitats represent a promising source of bioactive substances. Here, we report on the isolation, identification, productivity enhancement and application of the bioactive compounds of Streptomyces qinglanensis H4. Eighteen marine actinomycetes were isolated and tested for resistance to seven bacterial diseases. Using 16S rRNA sequencing analysis (GenBank accession number MW563772), the most powerful isolate was identified as S. qinglanensis. Although the strain produced active compound(s) against a number of Gram-negative and Gram-positive bacteria, it failed to inhibit pathogenic fungi. The obtained inhibition zones were 22.0 ± 1.5, 20.0 ± 1, 16.0 ± 1, 12.0 ± 1, 22.0 ± 1 and 24.0 ± 1 mm against Bacillus subtilis ATCC 6633, Escherichia coli ATCC 19404, Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 9027, Candida albicans ATCC 10231 and Staphylococcus aureus ATCC6538, respectively. To maximize bioactive compound synthesis, the Plackett-Burman design was used. The productivity increased up to 0.93-fold, when S. qinglanensis was grown in optimized medium composed of: (g/l) starch 30; KNO 3 0.5; K 2 HPO 4 0.25; MgSO 4 0.25; FeSO 4 •7H 2 O, 0.01; sea water concentration (%) 100; pH 8.0, and an incubation period of 9 days. Moreover, the anticancer activity of S. qinglanensis was tested against two different cell lines: HepG2 and CACO. The inhibition activities were 42.96 and 57.14%, respectively. Our findings suggest that the marine S. qinglanensis strain, which grows well on tailored medium, might be a source of bioactive substances for healthcare companies.

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“…are utilized to extend the half-lives of the molecules [119][120][121]. By embracing RSM to optimize media composition, researchers are enhancing the expression of designed molecules; the same method can also be used to enhance the production of nonconventional antibodies [122]. In addition, designing a polyvalent or polyspecific antibody structure by fusing several homounits or heterounits respectively improves both their efficacy and pharmacokinetics [123].…”

Section: Pluses and Minuses Of Non-conventional Antibodiesmentioning

confidence: 99%

Engineered polyspecific antibodies: A new frontier in the field of immunotherapeutics

Sandeep,

Shinde,

Ahmed

et al. 2023

Immunology

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The 21st‐century beginning remarked with the huge success of monospecific MAbs, however, in the last couple of years, polyspecific MAbs (PsAbs) have been an interesting topic and show promise of being biobetter than monospecific MAbs. Polyspecificity, in which a single antibody serves multiple specific target binding, has been hypothesized to contribute to the development of a highly effective antibody repertoire for immune defence. This polyspecific MAb trend represents an explosion that is gripping the whole pharmaceutical industry. This review is concerned with the current development and quality enforcement of PsAbs. All provided literature on monospecific MAbs and polyspecific MAbs (PsAbs) were searched using various electronic databases such as PubMed, Google Scholar, Web of Science, Elsevier, Springer, ACS, Google Patent and books via the keywords Antibody engineering, Polyspecific antibody, Conventional antibody, non‐conventional antibody, and Single domain antibody. In the literature, there are more than 100 different formats to construct PsAb by quadroma technology, chemical conjugation and genetic engineering. Till March 2023, nine PsAb have been approved around the world, and around 330 are in advanced developmental stages, showing the dominancy of PsAb in the growing health sector. Recent advancements in protein engineering techniques and the fusion of non‐conventional antibodies have made it possible to create complex PsAbs that demonstrate higher stability and enhanced potency. This marks the most significant achievement for cancer immunotherapy, in which PsAbs have immense promise. It is worth mentioning that seven out of the nine PsAbs have been approved as anti‐cancer therapy. As PsAbs continue to acquire prominence, they could pave the way for the development of novel immunotherapies for multiple diseases.

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Optimization of medium composition to increase the expression of recombinant human interferon-β using the Plackett–Burman and central composite design in E. coli SE1

Cited by 8 publications

References 36 publications

Enhancing docosahexaenoic acid production of Schizochytrium sp. by optimizing fermentation using central composite design

Enhancing docosahexaenoic acid production of Schizochytrium sp. by optimizing fermentation using central composite design

Bioactivity of Metabolites from Actinomycetes Isolates from Red Sea, Egypt

Engineered polyspecific antibodies: A new frontier in the field of immunotherapeutics

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