Optimization of mechanochemical-assisted extraction and decoloration by resins of polysaccharides from petals of Crocus sativus L

Abstract

Polysaccharide from petals of Crocus sativus (PPCs) was extracted by mechanochemical‐assisted extraction (MCAE) method. The optimization of MCAE conditions was applied by the Response surface methodology. The optimal conditions were as follows: milling for 8.14 min, keeping in the ultrasonic bath at 40 °C with NaOH (0.2 wt %) for 15 min, and the solid/liquid ratio of 1/30 g/mL. Under the conditions, a yield of 28.97% was obtained, which was very consistent with the predicted yield of 29.20%. The decolorization effects of crude PPCs were studied. HPD‐100 resin was selected as the decolorizing resin. The optimized dynamic adsorption condition was flow rate of 5.4 BV/hr, volume of 60 mL, temperature of 20 °C, 5 mg/mL PPCs extract. The ratios of decoloration, deproteinization, and polysaccharide recovery were 86.59, 86.60, and 80.54%, respectively. Then the antiproliferative activity of PPCs was assessed by 3H‐TdR method on human lung carcinoma A549 cell and breast cancer MCF7 cell. Practical applications Mechanochemical‐assisted extraction was applied to extract polysaccharides from petals of Crocus sativus, which was more time‐efficient with fewer solvents than traditional extraction methods. In addition, polysaccharides from petals of Crocus sativus demonstrated a promising antiproliferative property, which means that the byproducts of saffron might be a potential natural drug or health food for human lung carcinoma therapeutics.

Extraction of Natural Products by Direct Formation of Eutectic Systems

“…To verify the feasibility of this strategy, we used turmeric as the target sample and extracted curcumin, demethoxycurcumin, and bisdemethoxycurcumin based on DESys (Figure S1). Conventionally, when DESs are used to extract natural products from plants, ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE), and heating extraction (HE) as well as mechanochemical extraction (MCE) are also used in conjunction. − Thus, these methods can also be used for DESys-based extraction. Compared with other methods, MCE can induce a multidirectional simultaneous impact and cause cell destruction because of the use of small ceramic spheres.…”

Metabolites of medicine food homology-derived endophytic fungi and their activities

“…However, only a few metabolites of four types (polysaccharides, alkaloids, lignans and terpenoids) produced by the MFH plant-derived endophytic fungi have been reported to have the same or structurally related to those synthesized by their host plants in the recent five years. Crocus sativus petals are rich in polysaccharides ( Yang et al, 2018 ). Two homogeneous polysaccharides were isolated and purified from petals of C. sativus have been documented for their effects of antioxidant activity, which are potentially important for human health ( He et al, 2021 ).…”