Optimization of lipase production by Staphylococcus sp. Lp12

Abstract

A bacterial strain isolated from an oil contaminated soil, identified as Staphylococcus sp. Lp12 was screened for lipase activity on tributyrin agar and spirit blue agar medium. Maximum lipase production was observed at 48 h of growth (3.5 Eu/ml). Peptone was found to be as an ideal nitrogen source for production at a concentration of 1.0% (4.25 Eu/ml). Addition of any nitrogen source other than peptone to the medium resulted in a significant reduction of enzyme production. Lower lipase production was noted when an inorganic nitrogen source was used as the sole nitrogen source. Starch was used as a major carbon source for optimum production of lipase (4.25 Eu/ml) at a concentration of 1.5%. Of the natural oils, olive oil was able to induce more lipase (4.25 Eu/ml) rather than the oils like groundnut, coconut, castor oils. Basal medium containing tween 80 enhanced lipase production to a significant level. The pH 8 and temperature 45°C were found to be ideal pH and temperature for optimum production of lipase by this strain.

Characterization and Optimization of Lipase Production from Soil Microorganism (Serratia marcescens)

“…After shaking, 5ml of the suspension was transferred into a 25ml enrichment medium with the following composition: 1% olive oil, 10g/l peptone, 3g/l beef extract and 5g/l NaCl at pH 7.0 (Pallavi et al, 2010). The culture was incubated at 30ºC for 24 hours.1ml of the enriched culture suspension was serially diluted up to 10 -7 dilutions and plated onto nutrient agar plates.…”

“…The effects of 1% v/v of various oils on lipase production were investigated by replacing olive oil used in the basal medium for preliminary study with; crude oil, palm oil, soy oil and groundnut oil as described by Pallavi et al, (2010).…”

“…Five milliliters of the broth culture were inoculated into 250ml conical flasks each containing 100ml sterile production medium with ranging concentration (0.1-3.5% v/v) crude oil as described by Pallavi et al, (2010).…”

Molecular Identification, Production and Optimization of Lipase from Oil Contaminated Soil using Submerged Fermentation

“…Hydrolases include lipases, proteases, pectinases and carbohydrases [Fujii et al, 1986]. Lipases are fat splitting enzymes which require excess water to catalyze the formation of glycerol and fatty acids from long-chain triacylglycerol's by hydrolysis [Jaeger et al, 1994, Pallavi et al, 2010and Ram Reddy & Pallavi, 2012. Lipases produced by bacteria are used in dairy industries to hydrolyze milk fat, cheese ripening and flavor enhancement.…”

Efficient utilization of xylanase and lipase producing thermophilic marine actinomycetes (Streptomyces albus and Streptomyces hygroscopicus) in the production of ecofriendly alternative energy from waste

“…Lipases (triacyl glycerol acyl hydrolases, EC 3.1.1.3) catalyze the hydrolysis and the synthesis of esters formed from glycerol and long chain fatty acids. Lipases are used in detergent, food and agrochemical industries (Pallavi et al, 2010). The actinomycete *Corresponding author.…”