Optimization of Lipase Performance in Detergent Formulations for Hard Surfaces

Abstract

Lipases have been added to detergents in an attempt to develop more environmentally friendly and energy efficient products for triglyceride-based stains. However, more research is needed to improve the efficiency of lipase-containing detergents. In this work we have examined all the factors affecting lipase performance in detergent formulations, particularly for hard surface cleaning. Several issues, such as thermal and hydrodynamic stability, kinetic aspects, and surfactant compatibility, both in solution and at the oil/water interface, have been addressed and the conditions for an optimal performance have been established. The role of some stabilizers in preventing lipase deactivation has also been considered, and some insight on the mechanisms of lipase deactivation under washing conditions is provided. Finally, washing tests conducted in a continuous flow device which simulates a clean-in-place (CIP) system have demonstrated that, under optimal conditions, a reduction of the surfactant dosage was possible upon the incorporation of lipases in detergent formulations.

Waste Frying Oils as Substrate for Enzymatic Lipolysis: Optimization of Reaction Conditions in O/W Emulsion

“…As can be seen, a higher degree of hydrolysis was obtained at every time when GA was used to prepare the substrate emulsion. A higher inhibition of TLL activity with G600 than with GA was also observed in previous work by our research group [15], particularly when the surfactant was already present at the surface of the emulsion droplets. Other nonionic surfactants have also been reported to exert an inhibitory effect on lipase action [23,24].…”

“…The nonionic surfactant Glucopon 600 (G600) and gum arabic (GA), which proved to be compatible with lipase action in a previous work [15], were used as emulsifiers. Lipases (triacylglycerol acylhydrolases, EC 3.1.1.3) from three different origins were tried: (1) a fungal lipase from Thermomyces lanuginosus (TLL); (2) a bacterial one from Pseudomonas fluorescens (PFL); and one from animal origin, porcine pancreas (PPL).…”

“…The levels of each factor are shown in Table 1. The range for each variable was chosen on the basis of our experience from previous studies on the preparation of O/W emulsions for enzymatic hydrolysis [15,18]. The study was conducted with two different emulsifiers, G600 and GA.…”

Development of an Environmentally Acceptable Detergent Formulation for Fatty Soils Based on the Lipase from the Indigenous Extremophile Pseudomonas aeruginosa Strain

“…Studies on the function of lipase in oily soil removal from fabrics of different chemical accessibility confirmed that lipase increased the lipid removal in different fabrics and all morphological locations on the fiber, including fiber surfaces, interfiber capillaries, small capillaries and the center of the yarn bundle [29]. Furthermore, studies have confirmed the contribution of the incorporated lipases in hard-surface cleaning formulations in a packed bed column, indicating that the addition of lipases has a notable beneficial effect even in difficult-to-access porous substrates [30,31]. Since lipases incessantly perform chemical degradation of the soil and thereby constantly change the composition of the washing mixture, models that can predict and explain the activity of such formulas are difficult to obtain.…”

Imidazolium based ionic liquid type surfactant improves activity and thermal stability of lipase of Rhizopus oryzae

“…Surfactants have been widely used for various purposes in relation to lipases, as active ingredient in detergents along with lipase [10], for recovery of enzyme [11] and in some cases as an aid to stabilize and enhance the activity of lipases [12][13][14]. They can interact with the enzyme both in solution and interfaces.…”