Optimization of laccase production from a novel strain—Streptomyces psammoticus using response surface methodology

Niladevi, K.N.; Sukumaran, Rajeev K.; Jacob, Nicemol; Anisha, G.S.; Prema, P.

doi:10.1016/j.micres.2006.10.006

Cited by 100 publications

(50 citation statements)

References 20 publications

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“…The significance of each coefficient in the experimental model was also determined by T value and P value. A high T test value and a low P value indicate a high significance [75,76]. Student's t test of each coefficient of the model showed that the linear coefficient (X 2 ), the quadratic term (X 1 2 , X 2 2 , X 3 2 ) and the cross product (X 1 X 2 ) have significant effects (P > |T| < 0.05) on T. peruviana biodiesel (Y).…”

Section: Gas Chromatographic Analysis Of T Peruviana Seed Oilmentioning

confidence: 99%

Brette Pearl Spar Mable (BPSM): a potential recoverable catalyst as a renewable source of biodiesel from Thevetia peruviana seed oil for the benefit of sustainable development in West Africa

et al. 2018

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Background The energy requirements are globally on a rapid escalation, as technology advances, which is also true for a developing country like Nigeria, which is dependent on fossil fuels and its derivatives. Apart from its adverse effect on its economy, it has also negative impacts on the health and the environment, in general. However, investments in renewable energy are faced by the competitive oil prices, the very high investment cost for renewable energy, and high local electricity prices. This paper appraises the attractiveness of investing in renewable energy sources over the continued use of non-edible oil for electricity generation. Methods This paper explores the application of biomass seed oil to produce a renewable fuel (biodiesel) using heterogeneous base catalyst. Meanwhile, two-step processes were employed to produce the biofuel. In the first step (esterification), the acid value of the oil was reduced to the recommended limit (FFA ≤ 1.5) using H2SO4, while in the second step (transesterification), the catalyst calcination of grounded Brette Pearl Spar Mable (BPSM) pre-soaked in methanol was used as a biobase for biodiesel production. For the optimization, minitab response surface (MRS) and artificial neural network (ANN) were employed to model and optimize the process variables responsible for the optimum production of the oil and the biodiesel. Results The result presented showed that T. peruviana seed was found to be rich in oil with an average yield of 44.00% (w/w), and the oil was highly unsaturated with a high FFA. The maximum experimental biodiesel yield obtained was 86.00% at a catalyst amount of 4 g, a reaction time of 70 min, and a methanol/oil ratio of 0.1(v/v). This result was validated in triplicate under the same conditions, which yielded 85.70% (v/v) for MRS and 85.98% (v/v) for ANN. Furthermore, the optimization results also indicated that the p values (p < 0.05) of the model terms were significant, and the accuracy of the models achieved by MRS and ANN based on R2 depict that both optimization tools gave good predictions of R2 (MRS: R2 = 99.98% and ANN: R2 = 99.97%). The properties of the biodiesel, as described in other earlier reports using the same feedstock with different catalysts, indicated that the produced biodiesel had properties which agreed to those reported in the literature. Conclusions T. peruviana seed has proved to be a good biomass raw material for oil production, and its conversion to biofuel using a heterogeneous biobase catalyst showed its suitability as a renewable environmental friendly fuel. Government should invest in more sustainable sources of energy by imposing law for the use of non-edible oil or decreasing the price of electricity.

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Section: Gas Chromatographic Analysis Of T Peruviana Seed Oilmentioning

confidence: 99%

Brette Pearl Spar Mable (BPSM): a potential recoverable catalyst as a renewable source of biodiesel from Thevetia peruviana seed oil for the benefit of sustainable development in West Africa

et al. 2018

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“…They achieved a laccase yield of 55.4 U/g using an RSM approach. In our study, the maximum laccase yield of 607.2 U/mL was much higher than that obtained in other recent studies (Niladevi et al, 2009;Li et al, 2013). The rate of wheat straw degradation obtained in this study was also higher than that reported elsewhere.…”

Section: Optimization Of Fermentation Conditions (Rsm Ii)contrasting

confidence: 73%

Laccase production by Phomopsis liquidambari B3 cultured with food waste and wheat straw as the main nitrogen and carbon sources

Zhou

Yang

Mei

et al. 2014

Journal of the Air & Waste Management Association

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Food waste is the most difficult waste to manage because of the high treatment costs and the risk of environmental contamination. Wheat straw burning has become an increasingly discussed topic in recent years because of the air pollution it causes. However, food waste and wheat straw contain high amounts of nutrient elements; efficient utilization of these wastes can improve the environment by lessening airborne pollutant emission and the amount of waste that must be landfilled. It is well known that use of low-cost and abundant waste materials in microbial fermentations can reduce product costs. We aimed to use these resources while improving laccase production by the endophytic fungus Phomopsis liquidambari B3. We cultured P. liquidambari B3 in medium containing food waste and wheat straw as the main nitrogen and carbon sources, respectively. We optimized the fermentation conditions by response surface methodology (RSM), using a Box-Behnken design for RSM I and a central composite design for RSM II. Optimization resulted in an 11.07-fold (1.98-fold RSM I; 5.59-fold RSM II) increase in laccase yield compared with that before optimization. The model was validated by mathematical evaluations and by comparisons between predicted and experimental values. Under optimized conditions, 53.76% of lignin in wheat straw was degraded. By optimizing fermentation conditions and using multiple bioresources, laccase production by this fungus was increased. These results provide the foundation for future research and for scaled-up laccase production.Implications: Food waste and wheat straw are waste products, but they could be bioresources if they were managed properly. In this work, we innovatively used a mixture of food waste and wheat straw as a substrate for the novel strain Phomopsis liquidambari B3 to produce laccase. This process, which provided the foundation for the subsequent research and for scaled-up laccase production, offers solutions for both rural and urban pollution problems: that is, it reduces the amount of waste material that needs to be disposed of by burning or dumping, and it also produces a valuable product.

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“…The R-squared of 0.8875 revealed that the model could explain 88.75% of the variability in the response (Table 2). For a good statistical model, the R 2 should be in the range of 0.75-1.0 which indicates a good fit of the model [19]. The relatively high value of R 2 indicated that the quadratic equation could be used instead of an experimental system under the given conditions.…”

Section: Statistical Analysis and The Diagnostic Checking Of The Fittmentioning

confidence: 99%

Optimization of Key Factors Affecting Methane Production from Acidic Effluent Coming from the Sugarcane Juice Hydrogen Fermentation Process

2012

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Abstract:Response surface methodology with a central composite design was applied to optimize the key factors affecting methane production from the acidic effluent coming from the sugarcane juice hydrogen fermentation process. The parameters studied were substrate concentration, ratio of NaHCO 3 to substrate concentration and initial pH. The experimental results showed that substrate concentration and initial pH had significant individual (p < 0.05) effect on methane yield (MY). However, there was no interactive effect between these variables (p > 0.05). The maximum MY of 367 mL CH 4 /g-volatile solid (VS) added was obtained at the optimum conditions of 13,823 mg-COD/L, an NaHCO 3 to substrate concentration ratio of 3.09 and an initial pH of 7.07. Under the optimum conditions, MY was enhanced 4.4-fold in comparison to raw effluent.

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Optimization of laccase production from a novel strain—Streptomyces psammoticus using response surface methodology

Cited by 100 publications

References 20 publications

Brette Pearl Spar Mable (BPSM): a potential recoverable catalyst as a renewable source of biodiesel from Thevetia peruviana seed oil for the benefit of sustainable development in West Africa

Brette Pearl Spar Mable (BPSM): a potential recoverable catalyst as a renewable source of biodiesel from Thevetia peruviana seed oil for the benefit of sustainable development in West Africa

Laccase production by Phomopsis liquidambari B3 cultured with food waste and wheat straw as the main nitrogen and carbon sources

Optimization of Key Factors Affecting Methane Production from Acidic Effluent Coming from the Sugarcane Juice Hydrogen Fermentation Process

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