Optimization of Laccase Production from <i>Penicillium martensii </i>NRC 345

Abstract

Laccase production by Penicillium martensii NRC 345 was optimized. Eight media with different components were screened. The enzyme formed by P. martensii NRC 345 was detected mainly exocellularly under shake culture condition. Laccase formation reaches its maximum value with specific activities of about 7.18 U/mg protein at the twenty-sixth day of incubation at pH 5.5 and 30°C. Galactose (5 g/l) and sodium nitrate (0.2 g/l) were found to be the best carbon and nitrogen sources for laccase formation, respectively. Replacement of galactose instead of glucose at the same concentration increased laccase production by about more than ten-fold. Among the various wastes used wheat bran induces the highest laccase production with specific activity of 39.52 U/mg protein. Tween-80 and CuSO 4 .5H 2 O, were also tested for laccase induction.

Influence of Culture Conditions on Laccase Production, Growth, and Isoenzymes Patterns in Native White Rot Fungi from the Misiones Rainforest (Argentina)

“…These conditions were also reported for Schizophyllum commune at 28 ºC (Adejoye and Fasidi 2010) and Penicillium martensii at 30 ºC (Elshafei et al 2012). Similar behavior was also herein described for G. applanatum strain F, which was consistent with previous results by Echabarria and Gallon (2010), who found a maximum of Lac yield in Ganoderma sp.…”

Production of extracellular laccase from the newly isolated Bacillus sp. PK4

“…The type of carbon source in the medium plays a major role in the production of ligninolytic enzymes (Gaulhaup et al, 2002) and also the substance that induces the laccase production in one species may inhibit the same in other species (Elshafei et al, 2012). The carbon source of the growth medium appears to regulate the ligninolytic enzymes in microbes and the activity of these enzymes can be increased by the choice of the carbon source (Vaithanomsat et al, 2012).…”

Morphogenèse et fabrication d’une forme structurellement informée : expériences pédagogiques sur les systèmes funiculaires

“…Le développement de ces nouveaux outils a ouvert à la possibilité d'interopérabilité entre recherche morphologique des formes courbes et d'analyse structurelle, grâce à un flux d'informations continues dès les premières phases de conception (Elshafei et al, 2016). Cependant, il est important que les concepteurs sans connaissances approfondies des structures funiculaires soient capables d'interpréter correctement le processus de recherche de forme et ses résultats.…”