Optimization of kraft lignin decolorization and degradation by bacterial strain Bacillus velezensis using response surface methodology

Verma, Monika; Ekka, Amia; Mohapatra, Titikshya; Ghosh, Prabir

doi:10.1016/j.jece.2020.104270

Cited by 22 publications

(6 citation statements)

References 36 publications

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“…However, no laccase-producing B. aestuarii isolated from dye effluent streams using BFEFBW as a possible substrate has been reported. The 16S rRNA gene sequencing method is widely used to compare bacterial isolates that are closely related and those that are very distantly related (Verma et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Laccase production from Bacillus aestuarii KSK using Borassus flabellifer empty fruit bunch waste as a substrate and assessing their malachite green dye degradation

Selvam¹,

Ameen

Amirul

et al. 2022

Journal of Applied Microbiology

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Aims:The lignocellulosic waste, Borassus flabellifer empty fruit bunch waste (BFEFBW), was employed to produce laccase using Bacillus aestuarii KSK under solid-state fermentation (SSF) conditions and to assess the efficiency of malachite green (MG) dye decolourization. Methods and Results: Abiotic factors such as pH (5.0-9.0), temperature (25-45°C) and incubation time (24-96 h) were optimized using Response surface methodology-Box-Behenan Design (RSM-BBD) to exploit the laccase production. The anticipated model revealed that the highest laccase activity of 437 U/ml shows after 60 h of incubation at 35°C at pH 7.0. The bacterial laccase was used to remove 89% of the MG dye in less time. Conclusion:The laccase from B. aestuarii KSK decolorizes the MG and thereby making it a suitable choice for wastewater treatment from industrial effluents. Significance and Impact of the Study:This study is the first report on the production of laccase from B. flabellifer empty fruit bunch waste as a substrate. Bacillus aestuarii KSK was isolated from the soil sample and used to produce laccase under SSF conditions. The bacterial laccase has the potential for industrial application in textile waste dye treatment.

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Section: Discussionmentioning

confidence: 99%

Laccase production from Bacillus aestuarii KSK using Borassus flabellifer empty fruit bunch waste as a substrate and assessing their malachite green dye degradation

Selvam¹,

Ameen

Amirul

et al. 2022

Journal of Applied Microbiology

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“…For the effective decolorization of MO dye, central composite design (CCD) was used, and four different factors, i.e., pH, temperature, incubation time, and dye concentration, were considered for experimentation and the relationship between factors [ 43 ]. The four factors were divided into five levels (-α, −1, 0, +1, +α), and their relationship was determined by fitting the second-order polynomial equation [ 44 ]. Table 1 contains a list of the combinations of uncoded independent variables.…”

Section: Methodsmentioning

confidence: 99%

A feasible approach for azo-dye (methyl orange) degradation by textile effluent isolate Serratia marcescens ED1 strain for water sustainability: AST identification, degradation optimization and pathway hypothesis

Pandey,

Pathak,

Navneet

et al. 2024

Heliyon

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“…strain BL5 Alkali lignin 0.4%, 12 h Extent of lignin degradation: 27.04%; –OH bond stretching between alcohol and phenol; form ethers, phenols and alcohols [ 52 ] Bacillus cereus AH7-7 Sulfate lignin 1 g/L, 144 h Extent of lignin degradation: 25.90% [ 53 ] Bacillus aryabhattai Kraft lignin 0.5 g/L, 336 h The degradation extent was 84%. Absorbance changes at 280 nm [ 54 ] Bacillus cereus Kraft lignin 1 g/L, 72 h Extent of lignin degradation: 89%; decolorization extent: 40% [ 19 ] Bacillus subtilis TR-03 Alkali lignin 2 g/L, 36 h Extent of lignin degradation: 26.72%; decolorization extent: 71.23%; 280 nm absorbance increased; aromatic skeleton spectrum band vibration [ 55 ] Bacillus cereus TR-25 Alkali lignin 2 g/L, 36 h Extent of lignin degradation: 23%; decolorization extent: > 50%; 280 nm absorbance increased; aromatic skeleton spectrum band vibration Bacillus subtilis S11Y Alkaline lignin –, 72 h Extent of lignin degradation: 20% [ 46 ] Bacillus velezensis TSB1 Kraft lignin 0.6 g/L, 144 h Extent of lignin degradation: 40.39%; extent of lignin degradation: 56.16% [ 56 ] Bacillus spinoides Paenibacillus glucanolyticus SLM1 Bioselective lignin 0.2%, 400 h …”

Section: Lignin-degrading Bacteriamentioning

confidence: 99%

Bacterial transformation of lignin: key enzymes and high-value products

Gu,

Qiu,

et al. 2024

Biotechnol Biofuels

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Lignin, a natural organic polymer that is recyclable and inexpensive, serves as one of the most abundant green resources in nature. With the increasing consumption of fossil fuels and the deterioration of the environment, the development and utilization of renewable resources have attracted considerable attention. Therefore, the effective and comprehensive utilization of lignin has become an important global research topic, with the goal of environmental protection and economic development. This review focused on the bacteria and enzymes that can bio-transform lignin, focusing on the main ways that lignin can be utilized to produce high-value chemical products. Bacillus has demonstrated the most prominent effect on lignin degradation, with 89% lignin degradation by Bacillus cereus. Furthermore, several bacterial enzymes were discussed that can act on lignin, with the main enzymes consisting of dye-decolorizing peroxidases and laccase. Finally, low-molecular-weight lignin compounds were converted into value-added products through specific reaction pathways. These bacteria and enzymes may become potential candidates for efficient lignin degradation in the future, providing a method for lignin high-value conversion. In addition, the bacterial metabolic pathways convert lignin-derived aromatics into intermediates through the “biological funnel”, achieving the biosynthesis of value-added products. The utilization of this “biological funnel” of aromatic compounds may address the heterogeneous issue of the aromatic products obtained via lignin depolymerization. This may also simplify the separation of downstream target products and provide avenues for the commercial application of lignin conversion into high-value products.

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Optimization of kraft lignin decolorization and degradation by bacterial strain Bacillus velezensis using response surface methodology

Cited by 22 publications

References 36 publications

Laccase production from Bacillus aestuarii KSK using Borassus flabellifer empty fruit bunch waste as a substrate and assessing their malachite green dye degradation

Laccase production from Bacillus aestuarii KSK using Borassus flabellifer empty fruit bunch waste as a substrate and assessing their malachite green dye degradation

A feasible approach for azo-dye (methyl orange) degradation by textile effluent isolate Serratia marcescens ED1 strain for water sustainability: AST identification, degradation optimization and pathway hypothesis

Bacterial transformation of lignin: key enzymes and high-value products

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