Optimization of key factors affecting hydrogen production from food waste by anaerobic mixed cultures

Sreela-or, Chakkrit; Imai, Tsuyoshi; Plangklang, Pensri; Reungsang, Alissara

doi:10.1016/j.ijhydene.2011.04.136

Cited by 94 publications

(47 citation statements)

References 54 publications

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“…One of the disadvantages of this method is underestimation of the true bacterial diversity due to the fact that only the most prominent DGGE bands are analyzed. Various studies have shown that DGGE bands representing LAB are one of the most dominant bands (Fang et al, 2002;Kim et al, 2006;Li et al, 2006;Wu et al, 2006;Hung et al, 2007;Ren et al, 2007;Jo et al, 2007;Lo et al, 2008;Sreela-or et al, 2011). Another method of analyzing the biodiversity of hydrogen-producing consortia is cloning and sequencing of the 16S rDNA gene amplified on the total DNA isolated from the culture probes.…”

Section: Lactic Acid Bacteria -Influence On Hydrogen Producersmentioning

confidence: 99%

“…Cessation of hydrogen generation by LAB was suggested to be due to (i) substrate competition and/or (ii) excretion of bacteriocins inhibiting growth of other bacteria. These observations derive from examinations of both batch (Sreela-or et al, 2011) and continuous (Ren et al, 2007;Jo et al, 2007) mixed cultures as well as co-cultures where one component was a representative of clostridia and the second one of lactic acid bacteria (Noike et al, 2002). Heat treatment was proposed as a method of eliminating lactic acid bacteria (Noike et al, 2002;Baghchehsaraee et al, 2008).…”

Section: Et Al 2006mentioning

confidence: 99%

“…Substrate competition includes changes in the type of fermentation occurring in the bioreactors during long-term continuous processes and replacement of hydrogen fermentation by lactic acid or ethanol fermentation (Noike et al, 2002;Jo et al, 2007;Ren et al, 2007;Sreela-or et al, 2011). In all of the studies decrease in hydrogen production was observed with simultaneous increase of lactic acid and ethanol concentrations in the effluents or fluid phase of the culture.…”

Section: Et Al 2006mentioning

confidence: 99%

See 2 more Smart Citations

Lactic Acid Bacteria in Hydrogen-Producing Consortia: On Purpose or by Coincidence?

Sikora¹,

Błaszczyk²,

Jurkowski³

et al. 2013

Lactic Acid Bacteria - R &Amp; D for Food, Health and Livestock Purposes

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Section: Lactic Acid Bacteria -Influence On Hydrogen Producersmentioning

confidence: 99%

Section: Et Al 2006mentioning

confidence: 99%

Section: Et Al 2006mentioning

confidence: 99%

See 1 more Smart Citation

Lactic Acid Bacteria in Hydrogen-Producing Consortia: On Purpose or by Coincidence?

Sikora¹,

Błaszczyk²,

Jurkowski³

et al. 2013

Lactic Acid Bacteria - R &Amp; D for Food, Health and Livestock Purposes

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“…This UASB reactor was used to produce biogas from wastewater of cassava starch production process (Kalasin province, Thailand). The UASB granules were boiled at 100°C for 3 h to inactivate the hydrogentrophic methanogens (Sreela-or et al 2011). The hydrogen-producing bacteria in UASB granules were enriched by cultivating 200 g of heat-treated UASB granules in the 100 mL serum bottle containing 5 g/L xylose and 5 g/L glucose as carbon sources and supplemented with sufficient inorganic nutrients for bacterial growth (Lin & Lay 2005).…”

Section: Anaerobic Seed Sludge and Inoculum Preparationmentioning

confidence: 99%

Biohydrogen Productions from Hydrolysate of Water Hyacinth Stem (Eichhornia crassipes) Using Anaerobic Mixed Cultures

Pattra¹,

Sittijunda²

2017

JSM

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Response surface methodology (RSM) with central composite design (CCD) was applied to optimize key factors affecting hydrogen production (HP Kaedah permukaan tindak balas (RSM) dan pusat reka bentuk tergubah (CCD) digunakan untuk mengoptimumkan faktor utama yang mempengaruhi pengeluaran hidrogen (HP) daripada hidrolisat asid cair batang keladi bunting (WHS) melalui enapcemar anaerobik terawat-haba di dalam proses penapaian kumpulan. Faktor utama yang mempengaruhi kepekatan substrat dan pH awal dikaji. Keputusan menunjukkan bahawa kepekatan substrat dan pH awal telah memberi kesan

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“…beverages, fermented dairy products) [9][10][11] , renewable fuels (e.g. hydrogen, ethanol) 12,13 , pharmaceuticals (e.g. antibiotics, diagnostic markers) 14,15 , and industrial chemicals (e.g.…”

Section: Statement Of Part Of the Thesis Submitted To Qualify For Thementioning

confidence: 99%

Metabolic mechanisms and regulation of mixed culture fermentation

Hoelzle¹

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Fermentation of waste streams is an emerging method for production of biofuels and commodity chemicals, and mixed-culture fermentation allows for robustness and flexibility in the range of waste streams that can be processed, and in varying product mix from the fermenter. However, mechanistic models of mixed culture fermentation are limited, with mixed culture fermentation generally resulting in acetate, butyrate, and ethanol, with even these production modes are not clearly understood. Results from pure-culture fermentation, as well as those from methanogenic reactors indicate the possibility to produce high-value lactate and propionate if the cellular mechanisms which control the expression of these production modes can be harnessed and understood. Lactate and propionate are key chemicals in production of bioplastics, pesticides, preservatives, and food additives, among many other uses. Their production by mixed cultures has been observed at high substrate concentrations (shock loads), and inconsistently at high pH. They are produced via metabolic pathways which branch off of the primary energy-generation pathways. In general, they are thought to be produced by microbes as a way to redirect excess carbon flow in transient states, and are also thought to be produced as an electron sink. In this work, both of these possible production influences are explored for steady state production of lactate and propionate.By increasing substrate from limiting to non-limiting quantities, and thereby increasing the carbon load on a fermentation system, lactate production was successfully achieved and maintained during steady state only when substrate was in excess. Substrate consumption at this point was 14.1±0.8 gCOD·L -1 , and lactate accounted for 25±1% of the total product COD. Upon return to substrate-limiting conditions, the process returned to butyrate production. This demonstrates the reversibility of this production mode. Taxonomic analysis via Illumina pyrosequencing of the 16S rRNA gene revealed that the microbial community experienced a shift from Megasphaera-dominant during butyrate production to Ethanoligenens-dominant during lactate production. Taxonomy was also regained upon return to substrate-limiting conditions. However, taxonomy alone could not explain the product shift because Ethanoligenens produces little to no lactate in cultured references. A mechanistic description of the product shift therefore required molecular analysis of the metabolic mechanisms, and this was achieved through metaproteomics via SWATH-MS.The evidence suggests that at high carbon loads, toxic methylglyoxal is formed in many microbe groups, including Megasphaera, due to limited processing capacity of the ii dihydroxyacetone-P intermediate of glycolysis. The methylglyoxal is converted into lactate for disposal. This detoxification need is abated upon return to substrate-limited conditions. The impact of redox stress was examined using cathodic electro-fermentation with soluble mediator chemicals ranging from slightly negati...

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Optimization of key factors affecting hydrogen production from food waste by anaerobic mixed cultures

Cited by 94 publications

References 54 publications

Lactic Acid Bacteria in Hydrogen-Producing Consortia: On Purpose or by Coincidence?

Lactic Acid Bacteria in Hydrogen-Producing Consortia: On Purpose or by Coincidence?

Biohydrogen Productions from Hydrolysate of Water Hyacinth Stem (Eichhornia crassipes) Using Anaerobic Mixed Cultures

Metabolic mechanisms and regulation of mixed culture fermentation

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