Optimization of jujube peel pigment multivesicular liposome formulation and evaluation of the protective effect on UVB‐induced photodamage

Abstract

Jujube peel pigment (JP), an ideal natural water‐soluble pigment extracted from jujube peel, mainly consists of flavonoids and possesses a wide range of physiological activities. In this study, JP‐loaded multivesicular liposomes (JP‐MVL) were prepared using the double emulsification method. JP‐MVL was characterized, and its encapsulation efficiency was determined using the UV‐Vis method. Furthermore, the release behavior and antioxidant capacity of JP‐MVL were evaluated in vitro. The results displayed in the structure of JP‐MVL were spherical with internal vesicles; the average particle size of JP‐MVL was 5.63 μm, and the zeta potential was −69.50 mV. Analysis of the release results indicated that the best‐fitting models in PBS (Phosphate buffered saline pH 7.4) and 0.9% NaCl were the Higuchi and first‐order kinetic models, respectively. The cytotoxicity of JP‐MVL at the appropriate concentrations was negligible and had a good protective effect against UVB‐induced photodamage.