Optimization of iron dosage for microalgal biomass production as a feedstock for biofuel

Das, Probir; Thaher, Mahmoud; Hakim, Mohammed Abdul Quadir Mohd Abdul; Jabri, Hareb Al; Alghasal, Ghamza Saed H.S.

doi:10.1080/17597269.2018.1510720

Cited by 9 publications

(4 citation statements)

References 29 publications

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“…In this study, A. platensis exhibited a decline in the total protein contents when the concentrations of FeSO 4 increased. This result is in accordance with the findings of Das et al [66], who demonstrated that the carbohydrate content in Tetraselmis sp. increased with the increase of iron concentrations, while the increased iron in the culture decreased the protein content in the marine microalgae Tetraselmis sp.…”

Section: The Effect Of Feso 4 Concentrations On Carbohydrate and Prot...supporting

confidence: 94%

Influence of Fe+2 on the biomass, pigments, and essential fatty acids of ‏Arthrospira platensis

El‐Sheekh

Salman

Grmasha

et al. 2022

Biomass Conv. Bioref.

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The effects of different ferrous sulfate (FeSO4) concentrations on the fatty acid profile of the blue-green alga Arthrospira platensis cultured in Zarrouk’s medium to produce biodiesel were studied. Different ferrous sulfate concentrations (0, 0.005, 0.01, 0.05, and 0.1 g/L) and 0.01 g/L were examined on the biochemical composition of the alga and fatty acids profile of A. platensis. Findings revealed that the highest and lowest yields of fatty acid were 0 and 0.1 g/L FeSO4, respectively. It was also noticed an increase of palmitic acid, oleic acid, linoleic acid, γ-linolenic acid, and docosahexaenoic acid when ferrous sulfate was between 0.05 and 0.1 g/L, while these fatty acids decreased at low concentration. Ferrous sulfate at a concentration of 0.1 g/L exhibited an increase and best yields in the following: growth rate and the shortest doubling time, chlorophyll-a, phycocyanin, allophycocyanin, phycobiliproteins, and carotenoids. Thus, increasing the FeSO4 concentration to 0.1 g/L has led to the increase in fatty acid individuals, which in turn, resulted in potential enhancement of the biodiesel production from A. platensis.

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Section: The Effect Of Feso 4 Concentrations On Carbohydrate and Prot...supporting

confidence: 94%

Influence of Fe+2 on the biomass, pigments, and essential fatty acids of ‏Arthrospira platensis

El‐Sheekh

Salman

Grmasha

et al. 2022

Biomass Conv. Bioref.

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“…Among them, iron could be of interest since it is closely related to the growth, metabolism, and photosynthetic activity of microalgae [ 56 , 57 ]. Although some works have explored the influence of this metal in the production of biomass [ 58 ], lipids [ 59 ], carbohydrates, and proteins [ 60 ] by microalgae, its impact on PHA production has remained largely unexplored. Ultimately, the physiology of each strain and the environmental conditions to which it is adapted, confer them different sensitivity to nutrient limitation [ 61 ] and resistance to environmental and nutritional stress, which determines its PHA producing capacities as a response to said constraints [ 62 ].…”

Section: Introductionmentioning

confidence: 99%

Accumulation of PHA in the Microalgae Scenedesmus sp. under Nutrient-Deficient Conditions

et al. 2020

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Traditional plastics have undoubted utility and convenience for everyday life; but when they are derived from petroleum and are non-biodegradable, they contribute to two major crises today’s world is facing: fossil resources depletion and environmental degradation. Polyhydroxyalkanoates are a promising alternative to replace them, being biodegradable and suitable for a wide variety of applications. This biopolymer accumulates as energy and carbon storage material in various microorganisms, including microalgae. This study investigated the influence of glucose, N, P, Fe, and salinity over the production of polyhydroxyalkanoate (PHA) by Scenedesmus sp., a freshwater microalga strain not previously explored for this purpose. To assess the effect of the variables, a fractional Taguchi experimental design involving 16 experimental runs was planned and executed. Biopolymer was obtained in all the experiments in a wide range of concentrations (0.83–29.92%, w/w DW), and identified as polyhydroxybutyrate (PHB) by FTIR analysis. The statistical analysis of the response was carried out using Minitab 16, where phosphorus, glucose, and iron were identified as significant factors, together with the P-Fe and glucose-N interactions. The presence of other relevant macromolecules was also quantified. Doing this, this work contributes to the understanding of the critical factors that control PHA production and present Scenedesmus sp. as a promising species to produce bio-resources in commercial systems.

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“…Fe 3+ is one of the most appropriate heavy metals used to increase the lipid content in microalgae [90]. Interestingly, the addition concentration of Fe has not only improve the lipid content but could also increase the cell growth in green algae [67,91].…”

Section: Effects From the Combination Of Phosphorus Limitation And Bg...mentioning

confidence: 99%

Enhancement of biofuel production in microalgae by genetic and physiological modification

Pattharaprachayakul

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Microalgae are photosynthetic microorganisms, which could be used as potential microbial cell factories by directly converting CO2 into valuable bioproducts and biofuels. This study aims to improve the target biofuel feedstocks from the isolated green alga Chlorella sp. and the engineered cyanobacterium Synechococcus elongatus PCC 7942 in terms of the improvement on lipids (as precursors of FAMEs) and terpene (?-farnesene), respectively. The first part is concerned with the enhancement of lipids and the determination of FAMEs and biodiesel properties in Chlorella sp. under the phosphorus (P) limitation (0-50%) alone or in combination with heavy metals (Fe, Co, Pb) supplementation. The results showed that the highest yield of lipids was achieved under 0%P with 17 mM Co addition with 19% higher than the control. Moreover, the addition of low Pb concentrations could elevate the cell growth even under P limitation whereas the MUFAs, particularly palmitoleic acid (C16:1), was higher than PUFAs under most conditions. The overall biodiesel properties of the obtained FAMEs were of acceptable quality according to the standards (ASTM and EN). Additionally, the energy conversion from light energy to lipids was shown to be in the range of 10-16% conversion efficiency within 7 days, which corresponded to mixotrophic condition of microalgae cultures from previous studies. Hence, the physiological modification by stress treatments to cultures could offer the improvement of lipid content in microalgae although the genome database was not analyzed. Cyanobacterium S. elongatus PCC 7942 with available genomic database has advantages on genetic manipulation over the isolated Chlorella sp., thus the genetic modification was performed on S. elongatus PCC 7942 in this study. Herein, S. elongatus PCC 7942 was metabolically engineered for an enhanced production of ?-farnesene by optimizing the ribosome-binding site (RBS) of the codon-optimized farnesene synthase gene. The production of ?-farnesene was found to be enhanced in strains with a low translation initiation rate, resulting in ?-farnesene productivity of 0.57 mg/L/day. Using the RBS variants and random mutation, the fluorescence-based analysis was done on cells grown in 96-well culture plates to screen the ?-farnesene-producing strains, and the results showed no improvement in the titers by the RBS-optimized strains. However, evolutionary engineering of the RBS-optimized strains resulted in a two-fold increase in ?-farnesene productivity (1.2 mg/L/day) compared to the previous study. Therefore, combining metabolic and evolutionary engineering might be helpful for enhancing the cellular fitness of cyanobacteria for the production of target chemicals.

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Optimization of iron dosage for microalgal biomass production as a feedstock for biofuel

Cited by 9 publications

References 29 publications

Influence of Fe+2 on the biomass, pigments, and essential fatty acids of ‏Arthrospira platensis

Influence of Fe+2 on the biomass, pigments, and essential fatty acids of ‏Arthrospira platensis

Accumulation of PHA in the Microalgae Scenedesmus sp. under Nutrient-Deficient Conditions

Enhancement of biofuel production in microalgae by genetic and physiological modification

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