Optimization of in vitro solid culture of Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH hybrid strain

Ulu, Tufan Can; Susurluk, Alper

doi:10.16970/entoted.1004749

Cited by 2 publications

(1 citation statement)

References 40 publications

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“…EPNs are effectively used against a broad host range of pests (Askar et al, 2023;Dede et al, 2023). They can be mass-produced in fermenters and on solid media, and they are potent alternatives to chemicals used for below ground pests, where pesticides mostly fail (Shapiro-Ilan et al, 2012;Devi, 2018;Şahin et al, 2018;Ulu and Susurluk, 2021).…”

Section: Introductionmentioning

confidence: 99%

Evaluating a Portable Method and Two Irrigation Drippers for Field Application of Entomopathogenic Nematodes

Erdoğan,

Ulu

2024

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi

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Entomopathogenic nematodes (EPNs) can be applied using drip irrigation systems. However, the choice of driplines and types of drippers significantly impacts the efficacy of field applications. This study investigated the performance of EPN applications using two common dripper types (katif and cylindrical drippers) under both pot and field conditions. The primary objective of the study was to optimize EPN applications and create a modular system in which driplines and drippers can be selected based on the target pest or plant. In our modular system, driplines were connected to a battery-powered backpack sprayer rather than an irrigation system. The efficacy of EPN applications was assessed on Galleria mellonella L. (Lepidoptera: Pyralidae) larvae at a commercial dose of approximately 50 IJs cm-2. The results revealed that only 60% of the nematodes were discharged from the cylindrical drippers, with 40% becoming trapped in the irrigation system. In contrast, over 90% of the nematodes were successfully discharged from the katif dripper. As a result, the katif dripper exhibited significantly higher larval mortality compared to all other application methods. These findings emphasize the substantial impact of the dripper type on EPN discharge, while also highlighting the applicability of the modular method for EPN applications.

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Section: Introductionmentioning

confidence: 99%

Evaluating a Portable Method and Two Irrigation Drippers for Field Application of Entomopathogenic Nematodes

Erdoğan,

Ulu

2024

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi

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Efficiency of temperature and storage duration on some morphological measurements and reproductive capacity of the entomopathogenic nematode Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae)’s Turkish HBH hybrid strain

BÜTÜNER,

SUSURLUK

2023

TED

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Entomopathogenic nematodes (EPNs) are successfully used in the biological control of agricultural insect pests. This study aims to determine the body length of hermaphrodite individuals, egg diameter and reproductive capacity obtained from Infective Juveniles (IJs) stored at different temperatures and durations. Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae)’s Hybrid Strain HBH was used in the study. IJs stored at 15, 25 and 35°C for 7, 14 and 21 days were inoculated onto Galleria mellonella L., 1758 (Lepidoptera: Pyralidae) last instar larvae at a dose of 100 IJs. On the 2nd day of infection, hermaphrodite individuals and eggs were obtained by dissecting the larvae. The reproductive capacity was determined 10-12 days after infection. The study was conducted in Bursa Uludağ University, Faculty of Agriculture, Plant Protection Department, Nematology Laboratory in 2023. In conclusion, the longest hermaphrodite individuals and egg diameter were obtained as 6207.22 µm and 55.65 µm, respectively from the IJs stored for 7 days at 15°C. The highest reproductive capacity was also observed as 167.500 IJs per G. mellonella larva in IJs stored under the same conditions with respect to temperature and time. This study is important for assessing the morphological effects of different temperature values and storage durations on EPNs.

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Optimization of in vitro solid culture of Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH hybrid strain

Cited by 2 publications

References 40 publications

Evaluating a Portable Method and Two Irrigation Drippers for Field Application of Entomopathogenic Nematodes

Evaluating a Portable Method and Two Irrigation Drippers for Field Application of Entomopathogenic Nematodes

Efficiency of temperature and storage duration on some morphological measurements and reproductive capacity of the entomopathogenic nematode Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae)’s Turkish HBH hybrid strain

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