Optimization of in vitro propagation protocol of Dendrobium heterocarpum Wall. ex. Lindl. and clonal genetic fidelity assessment of the regenerates: An orchid of horticultural and medicinal importance

Longchar, Temjennokcha B.; Deb, Chitta Ranjan

doi:10.1016/j.sajb.2022.05.058

Cited by 18 publications

(6 citation statements)

References 29 publications

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“…VW and KC have more potassium phosphate monobasic content though the composition of potassium nitrate is lower than in MS medium [46]. MS and KC media have been extensively used for efficient seed germination and in vitro propagation of several orchids [47][48][49][50]. VC medium, on the other hand, has been mainly utilized for the proliferation of protocorm-like bodies, which helped in the orchid clonal multiplication on a large scale [51].…”

Section: Orchid Micropropagation Using Semi-solid Mediamentioning

confidence: 99%

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Nongdam

Beleski

Tikendra

et al. 2023

Plants

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Orchids, with their astonishingly stunning flowers, dominate the international floricultural market. They are considered prized assets for commercial applications in pharmaceutical and floricultural industries as they possess high therapeutic properties and superior ornamental values. The alarming depletion of orchid resources due to excessive unregulated commercial collection and mass habitat destruction makes orchid conservation measures an extreme priority. Conventional propagation methods cannot produce adequate number of orchids, which would meet the requirement of these ornamental plants for commercial and conservational purposes. In vitro orchid propagation using semi-solid media offers an outstanding prospect of rapidly producing quality plants on a large scale. However, the semi-solid (SS) system has shortcomings with low multiplication rates and high production costs. Orchid micropropagation using a temporary immersion system (TIS) overcomes the limitations of the SS system by reducing production costs and making scaleup and full automation possible for mass plant production. The current review highlights different aspects of in vitro orchid propagation using SS and TIS and their benefits and drawbacks on rapid plant generation.

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Section: Orchid Micropropagation Using Semi-solid Mediamentioning

confidence: 99%

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Nongdam

Beleski

Tikendra

et al. 2023

Plants

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“…In spite of the advantages, somaclonal variations induced by prolonged clonal propagation and cryptic genetic effects under microenvironment become the major limitations for maintaining certain desired traits [ 200 ]. Therefore, molecular markers have been applied to examine the genetic variability of in vitro raised plants to ensure clonal fidelity [ 201 , 202 ]. In this context, it has been pinpointed that biotechnological approaches will be a promising tool for orchid micropropagation and simultaneously, commercial production.…”

Section: Biotechnology-aided Orchid Productionmentioning

confidence: 99%

Advances and prospects of orchid research and industrialization

Zhang

Zhao

et al. 2022

Horticulture Research

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Orchidaceae is one of the largest, most diverse families in angiosperms with significant ecological and economical values. Orchids have long fascinated scientists by their complex life histories, exquisite floral morphology and pollination syndromes that exhibit exclusive specializations than any other plants on earth. These intrinsic factors together with human influences also make it a keystone group in biodiversity conservation. The advent of sequencing technologies and transgenic techniques represents a quantum leap in orchid research, enabling molecular approaches to be employed to resolve the historically interesting puzzles in orchid basic and applied biology. To date, 15 different orchid genomes covering four subfamilies (Apostasioideae, Vanilloideae, Epidendroideae and Orchidoideae) have been released. These genome projects have given rise to massive data that greatly empowers the studies pertaining to key innovations and evolutionary mechanisms for the breadth of orchid species. The extensive exploration of transcriptomics, comparative genomics, and recent advances in gene engineering have linked important traits of orchids with a multiplicity of gene families and their regulating networks, providing great potential for genetic enhancement and improvement. In this review, we summarize the progress and achievement in fundamental research and industrialized application of orchids with a particular focus on molecular tools, and make future prospects of orchid molecular breeding and post-genomic research, providing a comprehensive assemblage of start-of-art knowledge in orchid research and industrialization.

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“…The assessment of genetic fidelity between the micropropagated plantlets and their mother plants using molecular markers such as Sequence-related amplified polymorphism (SRAP) and Start codon targeted polymorphism (SCoT) serve as valuable tools to test the uniformity of plant material [50][51][52][53].…”

Section: Introductionmentioning

confidence: 99%

The Use of Wheat Starch as Gelling Agent for In Vitro Proliferation of Blackberry (Rubus fruticosus L.) Cultivars and the Evaluation of Genetic Fidelity after Repeated Subcultures

et al. 2023

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Micropropagation has an important role in the large-scale production of blackberry plant material, given the high proliferation rates of this species. The aim of the present study was to evaluate the proliferative capacity of blackberry grown in vitro on wheat starch-gelled culture medium compared to classical agar-gelled medium and to assess the genetic fidelity between the proliferated shoots in starch-gelled culture medium and their mother plants. Six blackberry varieties (‘Čačanska Bestrna’, ‘Chester Thornless’, ‘Driscoll’s Victoria’, ‘Loch Ness’, ‘Polar’, and ‘Karaka Black’) were tested. For the in vitro shoots proliferation, Murashige and Skoog (MS) medium supplemented with 0.5 mg dm−3 6-benzyladenine (BA) was used. The conventional medium was gelled with 0.5% plant agar, and wheat starch was used as an alternative gelling agent in a concentration of 5%. The results showed that for all blackberry cultivars, the highest number of shoots/inoculum was obtained in wheat starch-gelled culture medium, with a maximum value of 54.42 ± 4.18 presented by ‘Karaka Black’. Considering the length of the proliferated shoots, all tested cultivars presented outstanding results on the culture medium gelled with 5% wheat starch. The highest values regarding shoots length were observed on the ‘Chester Thornless’ followed by ‘Čačanska Bestrna’, and ‘Loch Ness’ with values of 5.55 ± 0.04 cm, 5.46 ± 0.06 cm, and 5.37 ± 0.09 cm, respectively. The genetic uniformity of the micropropagated shoots in relation to their mother plants was confirmed by sequence-related amplified polymorphism (SRAP) and start codon targeted (SCoT) molecular markers.

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Optimization of in vitro propagation protocol of Dendrobium heterocarpum Wall. ex. Lindl. and clonal genetic fidelity assessment of the regenerates: An orchid of horticultural and medicinal importance

Cited by 18 publications

References 29 publications

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Advances and prospects of orchid research and industrialization

The Use of Wheat Starch as Gelling Agent for In Vitro Proliferation of Blackberry (Rubus fruticosus L.) Cultivars and the Evaluation of Genetic Fidelity after Repeated Subcultures

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