Optimization of In-planta Method of Genetic Transformation  in Pigeon Pea (Cajanus cajan L. Millsp.)

Parray, Rouf Ahmad; Kaldate, Rahul; Chavan, R.L.

doi:10.20546/ijcmas.2019.806.008

Cited by 3 publications

(2 citation statements)

References 7 publications

(10 reference statements)

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“…Additional modifications include: (i) optimization of the Agrobacterium inoculum optical density (e.g. pigeon pea [141]); (ii) optimization of the acetosyringone concentration (e.g. tuberose/Polianthes tuberosa [142]); (iii) addition of a pre-culture step on Murashige and Skoog (MS) medium before inoculation (e.g.…”

Section: Shoot Apical Meristem Injury Under In Vivo Conditionsmentioning

confidence: 99%

A comprehensive review of in planta stable transformation strategies

Bélanger,

Copley,

Hoyos-Villegas

et al. 2024

Plant Methods

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Plant transformation remains a major bottleneck to the improvement of plant science, both on fundamental and practical levels. The recalcitrant nature of most commercial and minor crops to genetic transformation slows scientific progress for a large range of crops that are essential for food security on a global scale. Over the years, novel stable transformation strategies loosely grouped under the term “in planta” have been proposed and validated in a large number of model (e.g. Arabidopsis and rice), major (e.g. wheat and soybean) and minor (e.g. chickpea and lablab bean) species. The in planta approach is revolutionary as it is considered genotype-independent, technically simple (i.e. devoid of or with minimal tissue culture steps), affordable, and easy to implement in a broad range of experimental settings. In this article, we reviewed and categorized over 300 research articles, patents, theses, and videos demonstrating the applicability of different in planta transformation strategies in 105 different genera across 139 plant species. To support this review process, we propose a classification system for the in planta techniques based on five categories and a new nomenclature for more than 30 different in planta techniques. In complement to this, we clarified some grey areas regarding the in planta conceptual framework and provided insights regarding the past, current, and future scientific impacts of these techniques. To support the diffusion of this concept across the community, this review article will serve as an introductory point for an online compendium about in planta transformation strategies that will be available to all scientists. By expanding our knowledge about in planta transformation, we can find innovative approaches to unlock the full potential of plants, support the growth of scientific knowledge, and stimulate an equitable development of plant research in all countries and institutions.

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Section: Shoot Apical Meristem Injury Under In Vivo Conditionsmentioning

confidence: 99%

A comprehensive review of in planta stable transformation strategies

Bélanger,

Copley,

Hoyos-Villegas

et al. 2024

Plant Methods

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“…Pigeon pea is consumed all over the India and constitutes the main constituent of vegetarian diet. Several previous studies refers that India is the primary center of origin of pigeon pea [2][3][4][5]. Pulses are the major sources of protein among the vegetarians and it also provides essential amino acids, vitamins and minerals.…”

Section: Introductionmentioning

confidence: 99%

Influence of Micronutrient Managment on Growth and Yield Attributes in Pigeonpea [Cajanus cajan (L.) CV. PRG176] in Kalahandi District of Odisha

Malik

Naik

Sahoo

et al. 2021

JEAI

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Pigeon pea (Cajanus cajan (L.) is grown worldwide for its protein-rich seed. However, low availability of soil boron adversely affects the seed yield of pigeon pea. The present study was therefore conducted to assess the Influence of micronutrients mainly boron on crop growth and yield of pigeon pea (Cajanus cajan (L.) cv. PRG176. Field experiment was conducted at farmer’s field, Pipalpada and Boria of district Kalahandi of Odisha state. Boron as boric acid at 200, 300 and 400 ppm was given as foliar spray with 100% recommended dose of fertilizer (RDF) with ZnSO4 @ 25 kg/ha and plant height, growth rates and yield attributes were estimated. The results revealed that combined application of 100% RDF, ZnSO4 (25 kg/ha) and Boron (300 ppm) recorded the highest plant height (324.84 cm) at 180 days after sowing (DAS), highest dry matter accumulation (759.30g/m2) at 180 DAS and best crop growth rate (6.65 g/m2/day) during 90-180 DAS and highest relative growth rate (0.052 g/m2/day) during 30-90 DAS. Similarly these combination of treatment resulted in maximum number of branches plant-1 (10.30), pods branch-1 (19.67) pods plant-1 (202.33), seeds pod-1 (3.0) and grain yield (1702.64 kg ha-1), gross return (₹102150/ha), net return (₹ 61650/ha) and return per rupee investment (₹ 2.52) in pigeon pea cv.PRG176.

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