Optimization of immobilization conditions for Lactobacillus pentosus cells

Wang, Jianfei; Huang, Jiaqi; Guo, Huanyu; Jiang, Shaoming; Zhang, Jing; Ning, Yuchen; Fang, Mudannan; Liu, Shijie

doi:10.1007/s00449-020-02305-9

Cited by 16 publications

(11 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SA and PVA were gradually added to deionized water and dissolved with continuous agitation at 30 °C and 80 °C, respectively, and subsequently mixed to prepare SA-PVA hydrogel. The concentration of SA and PVA in mixed solution was 2.0% and 6.0%, respectively (Wang et al 2020). A specific amount of L. pentosus ATCC 8041 cells were inject into 100 mL sterilized SA-PVA hydrogel solution with continuous agitation.…”

Section: Cell Immobilizationmentioning

confidence: 99%

“…A specific amount of L. pentosus ATCC 8041 cells were inject into 100 mL sterilized SA-PVA hydrogel solution with continuous agitation. The fully mixed SA-PVA hydrogel solution containing L. pentosus ATCC 8041 cells were injected into the mixed cross-linking agent solution consisting of 0.10 M CaCl 2 and 2.5% H 3 BO 3 by an electrostatic droplet generator (Wang et al 2020;Poncelet et al 1999). Immobilized cell beads with the certain diameter were prepared with the diameter error of ± 0.3 mm.…”

Section: Cell Immobilizationmentioning

confidence: 99%

See 1 more Smart Citation

Optimization of immobilized Lactobacillus pentosus cell fermentation for lactic acid production

Wang

Huang

Laffend

et al. 2020

Bioresour. Bioprocess.

Self Cite

View full text Add to dashboard Cite

Parametric optimization is an effective way in fermentation process to improve product yield and productivity in order to save time, space and financial resources. In this study, Box-Behnken design was applied to optimize the conditions for lactic acid production by immobilized Lactobacillus pentosus ATCC 8041 cell fermentation. Two quadratic models and response surface methodology were performed to illustrate the effect of each parameters and their interactions on the lactic acid yield and glucose consumption rate in immobilized L. pentosus ATCC 8041 cell fermentation. The maximum lactic acid yield was obtained as 0.938 ± 0.003 g/g glucose with a productivity of 2.213 ± 0.008 g/ (L × h) under the optimized conditions of 2.0 mm bead diameter, 5.60 pH, 115.3 g/L initial glucose concentration, and 398.2 mg biomass (CDW) in 100 mL hydrogel. The analysis of variance indicated that the quadratic model was significant and could be used to scale up the fermentation process.

show abstract

Section: Cell Immobilizationmentioning

confidence: 99%

Section: Cell Immobilizationmentioning

confidence: 99%

Optimization of immobilized Lactobacillus pentosus cell fermentation for lactic acid production

Wang

Huang

Laffend

et al. 2020

Bioresour. Bioprocess.

Self Cite

View full text Add to dashboard Cite

show abstract

“…As shown in ANOVA (Table 3), the model was confirmed to be significant due to its F ‐value of 226.89 with a P ‐value of less than 0.0001 [16]. The model terms coded as

T

p H

n

N

T \times p H

T^{2}

p H^{2}

n^{2}

, and

N^{2}

were highly significant in this model because of their P ‐value of less than 0.05, which were considered to have the main influence on the biomass concentration in the batch fermentation of L. pentosus ATCC 8041 cells [17]. Other model terms with larger P ‐values were deemed to have minor effects on the biomass concentration.…”

Section: Resultsmentioning

confidence: 99%

“…The adjusted R 2 and predicted R 2 were 0.9864 and 0.9660, respectively, and the difference was less than 0.2, suggesting that the model value was highly accurate with a reasonable prediction [19]. The F ‐value of “lack of fit” was not significant, implying that the model was proper and reliable [16–20]. The adequate precision of 55.2309 was larger than 4, which indicated that this model was able to predict conditions of the biomass concentration in batch fermentation by L. pentosus ATCC 8041 cells with high precision [5].…”

Section: Resultsmentioning

confidence: 99%

Parametric optimization and kinetic study of l‐lactic acid production by homologous batch fermentation of Lactobacillus pentosus cells

Wang

Huang

Jiang

et al. 2020

Biotech and App Biochem

Self Cite

View full text Add to dashboard Cite

Parametric optimization always plays important roles in bioengineering systems to obtain a high product yield under the proper conditions. The parametric conditions of lactic acid production by homologous batch fermentation of Lactobacillus pentosus cells was optimized by the Box–Behnken design. The highest l‐lactic acid yield was obtained as 0.836 ± 0.003 g/g glucose with the productivity of 0.906 ± 0.003 g/(L × H) under the optimum conditions of 34.7 °C, pH 6.2, 148 rpm agitation speed, and 9.3 g/L nitrogen source concentration determined by quadratic response surface with high accuracy. The adequate kinetic models of cell growth rate, lactic production rate, and glucose consumption rate were also established to describe the fermentation behavior of L. pentosus cells with the correlation coefficients of 09985, 0.9990, and 0.9989, respectively.

show abstract

“…Diverse alternatives of substrates for probiotic growths have been explored as fruit juices, such as apple, orange, pomegranate, among others (Jaiswal and Abu-Ghannam 2013;Londoño et al 2015;Mousavi et al 2011;Pérez-Leonard and Hernández-Monzón 2015;Perricone et al 2014). However, few studies are focused in application of Taguchi´s methodology for probiotics growth optimization and these are relationship with indole-3-acetic acid production in symbiotic and non-symbiotic nitrogen-xing bacteria (genera Agrobacterium, Paenibacillus, Rhizobium, Klebsiella oxytoca, and Azotobacter) or immobilization conditions for Lactobacillus penntosus cells (Shokri and Emtiazi 2010;Wang et al 2020). Our study is the rst in describe the usage of juice from Parmentiera aculeata as substrate for probiotic growth and its optimization in Submerged Culture.…”

Section: Discussionmentioning

confidence: 99%

Valorization of Parmentiera aculeata juice in growth of probiotics in submerged culture and their postbiotic production: a first approach to healthy foods

et al. 2022

View full text Add to dashboard Cite

Actually, functional foods are good accepted for consumers for to improves health and new sources of substrates are explored. However, Parmentiera aculeate, which is a plant distributed in Mexico, has not explored. The P. aculeata juice was used as unique carbon source of promote the growth of two probiotic strains in Submerged Fermentation. Taguchi methodology with orthogonal array L9 was applied for its optimization. pH, agitation and inoculum concentration with three levels were evaluated and the best treatment was validated through a kinetic culture with monitoring some postbiotics. We observed an increase of 1.76 times in cellular concentration for Lactobacillus plantarum 14917 culture and the main produced postbiotic were short-chain fatty acids such as succinic, formic, acetic, propionic and lactic acids, which are associated the metabolisms probiotic and are important in human health. This study is the rst about valorization of Parmentiera aculeata juice as substrate for growth probiotic strain and future studies are necessaries for its application in functional food.

show abstract

Optimization of immobilization conditions for Lactobacillus pentosus cells

Cited by 16 publications

References 41 publications

Optimization of immobilized Lactobacillus pentosus cell fermentation for lactic acid production

Optimization of immobilized Lactobacillus pentosus cell fermentation for lactic acid production

Parametric optimization and kinetic study of l‐lactic acid production by homologous batch fermentation of Lactobacillus pentosus cells

Valorization of Parmentiera aculeata juice in growth of probiotics in submerged culture and their postbiotic production: a first approach to healthy foods

Contact Info

Product

Resources

About