Optimization of HPLC chromatographic conditions for determination of Transkarbam 12 and its degradation products

Pasáková, Ivana; Klimeš, Jiřı́; Sochor, J; Hrabálek, Alexandr

doi:10.1016/j.jpba.2006.03.014

Cited by 4 publications

(2 citation statements)

References 5 publications

(5 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other conditions included a 0.8-ml/min flow rate and a column temperature of 28˚C for a sample volume of 10 µl. The analyzed Sal-B contents were imaged and quantified using Empower System Suitability (24)(25)(26). Sal-B in S. miltiorrhiza Bge root was not used in the remaining experiments Purification and verification of Sal-B.…”

Section: Determination Of Sal-b In S Bowleyana Dunn Roots S Bowleyanamentioning

confidence: 99%

Salvia bowleyana Dunn root is a novel source of salvianolic acid B and displays antitumor effects against gastric cancer cells

et al. 2020

View full text Add to dashboard Cite

Salvianolic acid B (Sal-B) is widely used in China for the treatment of numerous diseases. Currently, Salvia miltiorrhiza Bunge is the main source of this compound, but Salvia bowleyana Dunn, a surrogate of S. miltiorrhiza Bge, may provide a novel source for obtaining more Sal-B. In the present study, a simple method for separation and purification of phenolic compounds from S. bowleyana Dunn roots was employed. Sal-B was subsequently purified and its inhibitory effect on the gastric cancer HGC-27 and AGS cell lines was investigated. Sal-B extracted from S. bowleyana Dunn displayed significant antitumor activity in proliferation and apoptosis assays. Overall, it was found that S. bowleyana Dunn has a higher Sal-B content than S. miltiorrhiza Bge and may be used as a novel source of this potential anti-gastric cancer compound.

show abstract

Section: Determination Of Sal-b In S Bowleyana Dunn Roots S Bowleyanamentioning

confidence: 99%

Salvia bowleyana Dunn root is a novel source of salvianolic acid B and displays antitumor effects against gastric cancer cells

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The gradient profile, with a flow rate of 0.550 mL/min, started with 98% A and 2% B, increasing to 100% B in 6 min, and, after keeping this ratio for 1.5 min, reversed into the initial conditions (Table A2). The QTRAP ® 6500 system was operated in Negative Electrospray Ionization Mode (ESI) (parameters used: gas temperature at 350 • C, curtain gas and collision gas pressure of 30 psi and Ionspray voltage of −4500 V), using Multiple Reaction Monitoring (MRM) scan mode, where Q1 and Q3 were set to more than one single mass allowing specific fragment ions from specific parent molecular ions to be detected [19]. The parameters for the ESI source and MRM are summarized in Tables A3 and A4.…”

Section: Instrumental Analysismentioning

confidence: 99%

Perfluoroalkyl Substance Assessment in Turin Metropolitan Area and Correlation with Potential Sources of Pollution According to the Water Safety Plan Risk Management Approach

et al. 2019

View full text Add to dashboard Cite

Per and polyfluoroalkyl substances (PFASs) are a huge class of Contaminants of Emerging Concern, well-known to be persistent, bioaccumulative and toxic. They have been detected in different environmental matrices, in wildlife and even in humans, with drinking water being considered as the main exposure route. Therefore, the present study focused on the estimation of PFAS in the Metropolitan Area of Turin, where SMAT (Società Metropolitana Acque Torino S.p.A.) is in charge of the management of the water cycle and the development of a tool for supporting “smart” water quality monitoring programs to address emerging pollutants’ assessments using multivariate spatial and statistical analysis tools. A new “green” analytical method was developed and validated in order to determine 16 different PFAS in drinking water with a direct injection to the Ultra High Performance Liquid Chromatography tandem Mass Spectrometry (UHPLC-MS/MS) system and without any pretreatment step. The validation of this analytical method resulted in really low Quantification Limits (5 ng L−1), in satisfying recoveries (70%–102%) and in a good linearity (R2 = 0.99) for every compound. Among the results, only 4 compounds and only 6% of the samples showed a pollution level higher than the limits of and Quantification (LOQ). Finally, the correlation study between the assessment findings and the industrial sites which serve as potential sources of pollution in the monitored area was carried out.

show abstract

Transkarbams: Transdermal Penetration-Enhancing Carbamates

Hrabálek

Vávrová

2015

Percutaneous Penetration Enhancers Chemical Methods in Penetration Enhancement

View full text Add to dashboard Cite

Optimization of HPLC chromatographic conditions for determination of Transkarbam 12 and its degradation products

Cited by 4 publications

References 5 publications

Salvia bowleyana Dunn root is a novel source of salvianolic acid B and displays antitumor effects against gastric cancer cells

Salvia bowleyana Dunn root is a novel source of salvianolic acid B and displays antitumor effects against gastric cancer cells

Perfluoroalkyl Substance Assessment in Turin Metropolitan Area and Correlation with Potential Sources of Pollution According to the Water Safety Plan Risk Management Approach

Transkarbams: Transdermal Penetration-Enhancing Carbamates

Contact Info

Product

Resources

About