Optimization of Horseradish Peroxidase Catalytic Degradation for 2-Methyl-6-Ethylaniline Removal Using Response Surface Methodology

Abstract

For optimizing the reaction conditions of 2-methyl-6-ethylaniline (MEA) degradation catalyzed by horseradish peroxidase (HRP), a response surface methodology with three factors and three levels was used in this research to establish a regression model, a ternary quadratic polynomial, in order to analyze temperature, H2O2 concentration and pH effects on MEA removal efficiency. The results showed that the regression model was significant (p < 0.0001), fitted well with experimental data and had a high degree of reliability and accuracy, and the data were reasonable with low errors. By analyzing interactions and solving the regression model, the maximum MEA removal efficiency was 97.90%, and the optimal conditions were defined as follows: pH 5.02, H2O2 concentration 13.41mM, and temperature 30.95 °C. Under the optimal conditions, the average MEA removal efficiency obtained from the experiments was 97.56%. This research can provide reference for the treatment of actual acetochlor industrial wastewater.

Removal of Persistent Sulfamethoxazole and Carbamazepine from Water by Horseradish Peroxidase Encapsulated into Poly(Vinyl Chloride) Electrospun Fibers

“…This might be due to partial inhibition and inactivation of horseradish peroxides at higher peroxide concentrations [ 34 ]. It should be highlighted that HRP is sensitive towards high hydrogen peroxide levels and undergoes inactivation after long-term contact with H 2 O 2 [ 35 ]. The horseradish peroxidase inactivation by the H 2 O 2 might occur due to two-side attack of the hydrogen peroxide on the HRP molecule.…”

In-situ H2O2 generation-on-demand based on ZnO2 nanoparticles for optical sensor systems involving peroxidase

“…[11] The pH range of maximum catalytic activity of peroxidase lies between 5 and 6 [12], whereas HRP activity retained between pH 5 and 10. [13][14][15] Among substrates, there are: aromatic amines [4], phenolic compounds [16], and some other substances [17], including immobilization of HRP in various polymer matrixes for determination of bioactive compounds [8] like hydrogen peroxide and organic peroxides [18], catecholamines and their metabolites [10], phenothiazines [19], protein biomarkers (such as Human IgG (HIgG)) [20] and biocomposites based on PDMS-SiO2NPs with co-localization/co-immobilization of enzyme (HRP or laccase) and substrate (3ethylbenzothiazoline-6-sulfonic acid (ABTS) or 3,3',5,5'teramethylbencidine (TMB)) to create a complete sensor platform or individual sensor elements. [21][22][23] HRP is widely used in laboratory work as an enzyme that catalyzes peroxidation processes, but at the same time it is an equally important analyte in medicine, chemical and pharmacological research, as well as in the biotechnological industry.…”

Agricultural waste as a source of peroxidase for wastewater treatment: Insight in kinetics and process parameters optimization for anthraquinone dye removal

“…dyes, phenols, etc.) present in wastewater (Shen et al, 2019;Ahirwar et al, 2017;Wagner and Nicell, 2005;Ely et al, 2017;Šekuljica et al, 2016). Šekuljica et al (2015) observed the oxidation of two anthraquinone dyes using commercial horseradish peroxidase, while Silva et al (2012) extracted peroxidase from turnip for decolorization of a reactive dye.…”