Optimization of Hormone for Artificial Breeding and Seed Production of Clarias batrachus (Linnaeus, 1758) Under Captive Condition

Abstract

Aims: The study aimed to develop brood stock of Magur (Clarias batrachus), artificial breeding and seed production under captive condition using growth hormones in north-eastern Bangladesh.
 Study Design: Fifteen pairs of brood Clarias batrachus were used in this experiment. Brood fishes were reared up to maturation for spawning operation in the farm by providing artificial diet for 4-5 months before onset of breeding season. In the present experiment, the brood fishes were induced with three growth hormones viz., Ovupin (100 mg dompridone and 0.2 mg S-GnRHa) at the rate of 1 ml.kg-1 body weight, Flash (20 mg S-GnRHa, 10 mg dompridone IP and propylene glycol) at the rate of 1 ml.kg-1 body weight and Human Chorionic Gonadotropin (HCG) hormone at the rate of 2272 IU.kg-1 body weight; respectively. The broods were injected with single injection in all treatments.
 Place and Duration of Study: The present study was conducted on induced spawning of Magur (Clarias batrachus) at the Reliance Aqua Farm, Boilor, Trisal of Mymensingh district from March to August, 2016.
 Methodology: Sexually mature healthy, uninjured male and female fishes were collected from the broodstock ponds for induced breeding. The fishes were transferred in the clean and germ free conditioning tanks for about 6-7 hours. During conditioning, continuous splash of water was given by keeping the male and female separate. The fishes were then weighed individually and injected intramuscularly with Ovupin, Flash and HCG hormone respectively. Only females were injected at 45° angle of the caudal peduncle. About 23-24 hours of post-injection the testes from males were dissected and mixed with stripping eggs for fertilization.
 Results: The incubation period for fertilized eggs were 23-24 hours for all the treatments. Fertilization rates were 78.20±0.52, 93.09±0.61 and 81.60±0.97 % and hatching rates were 54.93±0.61, 75.77±0.47 and 64.41±0.71 % in Ovupin, Flash and HCG treatments, respectively.
 Conclusion: This study revealed higher fertilization (93.09 %) and hatching (75.77 %) rate was achieved with Flash hormone. Therefore, Flash hormone could be effective for the artificial breeding and seed production of Magur (Clarias batrachus).

Artificial propagation of Asian catfish, Clarias batrachus (Linnaeus, 1758) in Asia: A review

“…Farmers maintained ideal sex ratio 1:2 (male and female) for higher rate of fertilization although sometimes it depends on the release of egg (Rajbongshi et al, 2020). Khan et al (2021) mentioned that magur fishes were cultured at a stocking density of 100 fish decimal -1 in the earthen ponds of the hatchery for broodstock development. The fishes were intensively cared by applying high protein (32%) commercial diet enriched with vitamin (A, B, C and E) twice daily at the rate of 3-5% body weight to make them sexually mature.…”

“…An experiment was also encountered while using HCG at the rate of 4000 IU/kg of body weight during breeding operation (Naser et al 1990). Khan et al (2021) conducted experiment to monitor the three growth hormones viz., Ovupin (100 mg dompridone and 0.2 mg S-GnRHa) at the rate of 1 ml.kg-1 body weight, Flash (20 mg S-GnRHa, 10 mg dompridone and propylene glycol) at the rate of 1 ml.kg-1 body weight and Human Chorionic Gonadotropin (HCG) hormone at the rate of 2272 IU.kg-1 body weight on breeding performances. Sahoo et al (Khan et al, 2021).…”

“…Fin fish hatchery was first established in Jessore by Mohoshin Master in 1967. Among the freshwater fishes, catfish is one of the most important groups of fish in this delta and is getting increasingly popular showing a promising future for commercial culture (Khan et al ,2021). Due to natural and manmade hazards, biodiversity of fish and other aquatic organisms in open water have been declining so much and with such rapidity that the aquatic animals, especially fish are unable to cope with (Mollah, 2005).…”

First Record of Induced Spawning of Magur (Clarias batratus) Without Sacrificing Male Fish in Bangladesh

“…To address the needs of small and medium-sized businesses and local farmers, it becomes vital to start producing and rearing magur larvae under controlled conditions. Few researchers have successfully employed induced spawning methods for C. batrachus to produce seeds utilizing a variety of natural and synthetic agents, including fish pituitary gland extracts, Human Chorionic Gonadotropin (HCG), ovaprim and ovatide, among others [7][8][9][10][11] . Although C. batrachus-induced breeding is not a tough task, the main issue is fertilization, hatching success and early fish development.…”