Optimization of high expression and purification of recombinant streptokinase and in vitro evaluation of its thrombolytic activity

El-Dabaa, Ehab; Okasha, Hend; Samir, Safia; El-Kalamawy, Hadeer Adel; Nasr, Sami Mohamed; Saber, Mohamed

doi:10.1016/j.arabjc.2022.103799

Cited by 4 publications

(2 citation statements)

References 25 publications

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“…After induction for 24 and 48 h, cultures were centrifuged at 7000 g and 4 °C and pellets were resuspended in 0.5 mL of PBS buffer and sonicated. The RNase expression was visualized using SDS-PAGE in comparison to uninduced control (El-Dabaa et al 2022 ).…”

Section: Methodsmentioning

confidence: 99%

Homemade plasmid Miniprep solutions for affordable research in low-fund laboratories

et al. 2022

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As a consequence of Covid-19 pandemic, the basic lab consumables are in shortage, especially in the low-income countries. Thus, the main objective of the present study is to develop and evaluate homemade solution to isolate plasmid. To pursue this objective, RNase A was overexpressed in Bl21 DE3 cells (E. coli strain) and prepared as crude refolding reaction with proper activity. Also, lysis buffers, neutralization buffer, and washing buffers were prepared. The homemade miniprep kit showed successful isolation of the px48SpCas9 plasmid. The prepared plasmid purity was enough to be used successfully in PCR amplification. In addition, to get extra benefits from this study, seven primers were designed to match the plasmid backbone to produce DNA ladder (100–1500 bp). In conclusion, we were able to have attainable working solutions for plasmid miniprep and DNA ladder.

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Section: Methodsmentioning

confidence: 99%

Homemade plasmid Miniprep solutions for affordable research in low-fund laboratories

et al. 2022

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“…Six male adult rabbits, weighing about 2.4 kg were used for the in vivo experiment. One week before use, the animals were acclimated to a conventional animal house (El-Dabaa et al, 2022).…”

Section: In Vivo Analysis Of Streptokinasementioning

confidence: 99%

Evaluation of clinical efficacy of streptokinase by comparison with the thrombolytic agent on animal model

Yousaf,

Arshad,

Harraz

et al. 2024

Braz. J. Biol.

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Cardiovascular disorders, including acute myocardial infarction (AMI), often lead to blood clot formation, impacting blood circulation. Streptokinase, a cost-effective and widely available thrombolytic agent, is crucial in treating thrombosis. This study aimed to produce streptokinase from Streptococcus pyogenes EBL-48 and compare its efficacy with heparin in an animal model. We evaluated the clot-lysing effectiveness of streptokinase produced from Streptococcus pyogenes EBL-48, emphasizing its low cost and ease of production. Streptokinase was produced using pre-optimized fermentation media and purified through ion exchange and gel-filtration chromatography. In vivo analysis involved inducing clots in a trial animal model using ferric chloride, comparing streptokinase with heparin. Ultrasonography assessed the clot-lysing activity of streptokinase. Streptokinase (47 kDa) effectively lysed clots, proving its low cost, easy production, and minimal adverse effects. Ultrasonography confirmed its fibrinolytic efficacy. These findings highlight potential as an affordable and easily produced thrombolytic agent, particularly relevant in resource-limited settings. Streptokinase efficacy and minimal adverse effects make it a promising option for thrombolytic therapy, especially in economically constrained regions. Future studies could optimize production techniques, explore different strains, and conduct clinical trials for human validation. Comparative studies with other thrombolytic agents would enhance understanding of their advantages and limitations.

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Microbial Production of r-DNA Products

Modasiya,

Mori,

Goswami

2024

Microbial Products for Health and Nutrition

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Optimization of high expression and purification of recombinant streptokinase and in vitro evaluation of its thrombolytic activity

Cited by 4 publications

References 25 publications

Homemade plasmid Miniprep solutions for affordable research in low-fund laboratories

Homemade plasmid Miniprep solutions for affordable research in low-fund laboratories

Evaluation of clinical efficacy of streptokinase by comparison with the thrombolytic agent on animal model

Microbial Production of r-DNA Products

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