Optimization of Growth Conditions and Biological Activities of Nepalese Ganoderma lucidum Strain Philippine

Abstract

Ganoderma lucidum has been extensively studied for its valuable medicinal importance. In this study, the artificial cultivation of G. lucidum strain Philippine in different culture media, including sawdust substrate, was performed and optimized on the Potato Dextrose Agar (PDA) media. Phytochemical, antibacterial, and antioxidant analyses were performed and compared between the ethanol extracts prepared from two different cultures (fruit from synthetic log culture and mycelia from PDA media culture). Both the 200 mg/mL and 100 mg/mL concentrations of extracts inhibited all the tested bacteria, and the results were promising than the corresponding control using antibiotics. The fruit extract showed higher antioxidant potential (150.6 ± 56.92 mg ascorbic acid equivalent/g extract) than mycelial extract (144.28 ± 81.72 mg ascorbic acid equivalent/g extract). The results indicate that fruiting bodies of G. lucidum cultivated in a complex dust medium possess higher antioxidant properties than mycelia culture, which can be further explored for therapeutic applications.

Optimization of mycelial growth and cultivation of wild Ganoderma sinense

“…The optimal pH for enhancing Ganoderma mycelial growth varies from one species to another. For example, the maximum mycelial growth of G. lucidum was observed at pH 4.5–5.5 (Jayasinghe et al, 2008 ; Subedi et al, 2021 ), whereas Ganoderma resinaceum and G. australe grew fastest at pH 7 and pH 7–8, respectively (Kim et al, 2006 ; Luangharn et al, 2017 ). For the growth of G. sinense , pH 7 proved to be optimal ( Fig.…”

The Efficacy of Ganoderma lucidum Extracts on Treating Endometrial Cancer: A Network Pharmacology Approach

“…Ganoderma , also known as Lingzhi, is a highly esteemed herb in traditional Chinese medicine (TCM) and has been widely employed as a functional food and therapeutic agent [ 4 ]. Ganoderma lucidum (GL), a prominent species within the Ganoderma genus, exhibits a diverse array of pharmacological properties, including antiviral, anti-inflammatory, and antioxidative activities [ 5 , 6 ]. Extensive research has revealed that GL displays remarkable antitumor effects against various types of cancers.…”