“…It was observed that GA production initiated on the first day of fermentation, which is contrary to reports that GA commences about 46 h after the initiation of fermentation following nitrogen depletion in the medium (Escamilla et al, 2000;Rodrigues et al, 2009;Rios-Iribe et al, 2011). However, Lale and Gadre (2010) also reported GA production at 20 h by a mutant of G. fujikuroi.…”
Section: Resultscontrasting
confidence: 70%
“…In addition to these, BP has been shown to be rich in essential amino acids, dietary fibre, polyunsaturated fatty acids, iron and potassium (Ibitoye, 2005;Juarez-Garcia et al, 2006). All these are relevant for a proper growth of the fermenting organisms and subsequent production of GA. Gibberellin production is reported to commence during the fermentation when nitrogen is exhausted in the medium and continues in the presence of adequate residual carbon in the substrate (Escamilla et al, 2000). Therefore, a good substrate should provide adequate nutrients for an initial mycelial growth in a nitrogen-limited but balanced medium (Rodrigues et al, 2009).…”
Section: Resultsmentioning
confidence: 99%
“…It not only affects the growth of the fungi, but also influences their physiological activities. Escamilla et al (2000) reported that high pH neutralizes acid production e.g. Le Chatelier's principle; therefore, the highest GA production is recorded at low C:N ratios and large pH.…”
The present study aimed to produce gibberellic acid through fermentation using banana (Musa sapientum) peel waste as substrate. Banana peel, a domestic and industrial waste, constitutes a potential source of cheap fermentable substrate for the production of other value-added products. Fusarium moniliforme ATCC 10052 and Aspergillus niger CBS 513.88 were used as fermenting organisms. The substrate was dried, ground and its proximate composition determined. The powdered substrate was added to a modified CzapekDox broth (a semisynthetic medium), with Carboxyl methylcellulose (CMC) as control. The fermentation conditions were: pH 5.5; inoculum size 1% (5 × 10 5 spores/mL F. moniliforme) (2 × 10 6 spores/mL A. niger); substrate concentration 2 g; temperature 25 ± 2 o C; fermentation time 7 days. The fermentation was optimized by varying pH, inoculum size, substrate concentration and fermentation time. The extracted GA was subjected to infra-red spectroscopy using FT-IR. The parameters which gave the highest GA yields were thereafter combined in a single fermentation. The results of proximate analysis of banana peel substrate revealed 8.65% moisture, 9.54% protein, 5.40% lipids, 11.45% ash, 22.34% crude fibre, and 42.62% carbohydrate. The GA yields of 13.55 g/L and 12.44 g/L were produced from the banana peel substrate and 3.62 and 2.61 g/L from the CMC control by F. moniliforme and A. niger respectively. Under optimized conditions, F. moniliforme produced 17.48 g/L GA, while A. niger produced 13.50 g/L. Extracted GA was similar to standard GA sample and the present results support the potential use of banana peel for fermentative GA production.
“…It was observed that GA production initiated on the first day of fermentation, which is contrary to reports that GA commences about 46 h after the initiation of fermentation following nitrogen depletion in the medium (Escamilla et al, 2000;Rodrigues et al, 2009;Rios-Iribe et al, 2011). However, Lale and Gadre (2010) also reported GA production at 20 h by a mutant of G. fujikuroi.…”
Section: Resultscontrasting
confidence: 70%
“…In addition to these, BP has been shown to be rich in essential amino acids, dietary fibre, polyunsaturated fatty acids, iron and potassium (Ibitoye, 2005;Juarez-Garcia et al, 2006). All these are relevant for a proper growth of the fermenting organisms and subsequent production of GA. Gibberellin production is reported to commence during the fermentation when nitrogen is exhausted in the medium and continues in the presence of adequate residual carbon in the substrate (Escamilla et al, 2000). Therefore, a good substrate should provide adequate nutrients for an initial mycelial growth in a nitrogen-limited but balanced medium (Rodrigues et al, 2009).…”
Section: Resultsmentioning
confidence: 99%
“…It not only affects the growth of the fungi, but also influences their physiological activities. Escamilla et al (2000) reported that high pH neutralizes acid production e.g. Le Chatelier's principle; therefore, the highest GA production is recorded at low C:N ratios and large pH.…”
The present study aimed to produce gibberellic acid through fermentation using banana (Musa sapientum) peel waste as substrate. Banana peel, a domestic and industrial waste, constitutes a potential source of cheap fermentable substrate for the production of other value-added products. Fusarium moniliforme ATCC 10052 and Aspergillus niger CBS 513.88 were used as fermenting organisms. The substrate was dried, ground and its proximate composition determined. The powdered substrate was added to a modified CzapekDox broth (a semisynthetic medium), with Carboxyl methylcellulose (CMC) as control. The fermentation conditions were: pH 5.5; inoculum size 1% (5 × 10 5 spores/mL F. moniliforme) (2 × 10 6 spores/mL A. niger); substrate concentration 2 g; temperature 25 ± 2 o C; fermentation time 7 days. The fermentation was optimized by varying pH, inoculum size, substrate concentration and fermentation time. The extracted GA was subjected to infra-red spectroscopy using FT-IR. The parameters which gave the highest GA yields were thereafter combined in a single fermentation. The results of proximate analysis of banana peel substrate revealed 8.65% moisture, 9.54% protein, 5.40% lipids, 11.45% ash, 22.34% crude fibre, and 42.62% carbohydrate. The GA yields of 13.55 g/L and 12.44 g/L were produced from the banana peel substrate and 3.62 and 2.61 g/L from the CMC control by F. moniliforme and A. niger respectively. Under optimized conditions, F. moniliforme produced 17.48 g/L GA, while A. niger produced 13.50 g/L. Extracted GA was similar to standard GA sample and the present results support the potential use of banana peel for fermentative GA production.
“…Thus, the optimization of inoculum production was performed where CP extract added of sucrose was chosen for cells' cultivation. Some patents that present the difficulty to produce 1-5 g of GA 3 /L are reported (Escamilla et al, 2000). In this work, a production 5.8 g of GA 3 /kg of CP was reached in 3 days even before any phisico-chemical optimization of the fermentation process.…”
Section: Discussionmentioning
confidence: 65%
“…If used separately, the substrates CB and SCB have not showed significant results of production under the conditions studied. The production of gibberellins starts when nitrogen is depleted and continues when a sufficient carbon concentration is available in the substrate (Escamilla et al, 2000). In this way, substrates must provide an initial mycelia growth in a nitrogen-limited balanced medium, the beginning of GA 3 production after nitrogen exhaustion and an extended metabolite production in the presence of sufficiently available carbon substrate.…”
Section: Screening Of Strains and Substrates For Ga 3 Productionmentioning
A nonstructured model was used to study the dynamics of gibberellic acid production in a stirred tank bioreactor. Experimental data were obtained from submerged batch cultures of Gibberella fujikuroi (CDBB H-984) grown in varying ratios of glucose-corn oil as the carbon source. The nitrogen depletion effect was included in mathematical model by considering the specific kinetic constants as a linear function of the normalized nitrogen consumption rate. The kinetics of biomass growth and consumption of phosphate and nitrogen were based on the logistic model. The traditional first-order kinetic model was used to describe the specific consumption of glucose and corn oil. The nitrogen effect was solely included in the phosphate and corn oil consumption and biomass growth. The model fit was satisfactory, revealing the dependence of the kinetics with respect to the nitrogen assimilation rate. Through simulations, it was possible to make diagrams of specific growth rate and specific rate of substrate consumptions, which was a powerful tool for understanding the metabolic interactions that occurred during the various stages of fermentation process. This kinetic analysis provided the proposal of a possible mechanism of regulation on growth, substrate consumptions, and production of gibberellic acid (GA3 ) in G. fujikuroi.
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