Abstract:Restriction isotyping is a convenient method to perform Apo E genotyping. However, some experimental conditions can affect the restriction isotyping prpcedure and can lead to results prone to misinterpretation. It was evaluated whether the concentration of teraplate DNA or different methods of DNA Isolation have an influence on restriction isotyping. Reproducible results were achieved with 0.05l ng genomic DNA in a 100 PCR mixture. Using less DNA resulted in the appearance of non-specific bands. This could lea… Show more
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