Optimization of genotypic and biochemical methods to profile P. acnes isolates from a patient population

Cebrián, Rubén; Arévalo, Sergio; Arias‐Santiago, Salvador; Riazzo, Cristina; Rojo, María Dolores; Bermudez, Pilar García; Valdivia, Eva; Martínez‐Bueno, Manuel; Maqueda, Mercedes

doi:10.1016/j.mimet.2017.07.008

Cited by 5 publications

(8 citation statements)

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“…Several clinical isolates of P . acnes (n = 20) from different sources (Table 1 ) including three antibiotic-resistant isolates, P3, P11 and P12 previously characterized 33 , were examined for susceptibility to the bacteriocin AS-48. The MIC was determined by the broth microdilution method according to the Clinical and Laboratory Standard Institute (CLSI) guidelines for anaerobic bacteria, although using DRCM broth to promote the growth.…”

Section: Resultsmentioning

confidence: 99%

“…No clear relation was observed between the antibiotic resistance of these strains and their subgroup profiles. Indeed, the P3, P11 and P12 strains from acne patiens previously treated with antibiotics, were resistant to erythromycin, while P11 and P12 showed combined resistance to clindamycin 33 . The remarkable susceptibility of these strains to AS-48 in liquid medium (Table 1 ), in the range of the most sensitive Gram-positive bacteria assayed, suggests that this peptide could be used to effectively inhibit clinical propionibacteria at wery low AS-48 concentrations (below 2.50 µg/mL).…”

Section: Discussionmentioning

confidence: 99%

“…In a recent study we have described the genotypic and phenotypic profile of several clinical isolates of P . acnes from different sources, which were grouped into two clusters by Random Amplification of Polymorphic DNA, in correspondence with the phylogroups I and II previously established 33 , 48 . Remarkably, all the isolates from acne and the majority from opportunistic infections belonged to biotype I-B3 in correlation with the phylotype IA1, which is involved in the pathophysiology of P .…”

Section: Discussionmentioning

confidence: 99%

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Control of Propionibacterium acnes by natural antimicrobial substances: Role of the bacteriocin AS-48 and lysozyme

Cebrián

Arévalo

Rubiño

et al. 2018

Sci Rep

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We report the high susceptibility of several clinical isolates of Propionibacterium acnes from different sources (skin, bone, wound exudates, abscess or blood contamination) to the head-to-tail cyclized bacteriocin AS-48. This peptide is a feasible candidate for further pharmacological development against this bacterium, due to its physicochemical and biological characteristics, even when it is growing in a biofilm. Thus, the treatment of pre-formed biofilms with AS-48 resulted in a dose- and time-dependent disruption of the biofilm architecture beside the decrease of bacterial viability. Furthermore, we demonstrated the potential of lysozyme to bolster the inhibitory activity of AS-48 against P. acnes, rendering high reductions in the MIC values, even in matrix-growing cultures, according to the results obtained using a range of microscopy and bioassay techniques. The improvement of the activity of AS-48 through its co-formulation with lysozyme may be considered an alternative in the control of P. acnes, especially after proving the absence of cytotoxicity demonstrated by these natural compounds on relevant human skin cell lines. In summary, this study supports that compositions comprising the bacteriocin AS-48 plus lysozyme must be considered as promising candidates for topical applications with medical and pharmaceutical purposes against dermatological diseases such as acne vulgaris.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Control of Propionibacterium acnes by natural antimicrobial substances: Role of the bacteriocin AS-48 and lysozyme

Cebrián

Arévalo

Rubiño

et al. 2018

Sci Rep

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“…The multiplex assay has now been described for the typing of P. acnes isolates from patients with acne [ 60 , 61 , 62 ], PMH [ 63 ], wound exudates and abscesses [ 62 ], microdiscectomy tissue [ 64 ], central nervous system and prosthetic joint infections [ 65 ], and other clinical conditions [ 60 ]. It has also been used for high throughput typing of P. acnes isolates from the pre-operative skin and surgical wound of patients undergoing spinal operations; this study was a large randomized controlled trial investigating the effects of povidone-iodine-alcohol (PVI) and chlorhexidine gluconate-alcohol on surgical wound contamination compared to PVI alone [ 66 ].…”

Section: Newer Molecular Typing Methodologies For Propiomentioning

confidence: 99%

“…It has also been used for high throughput typing of P. acnes isolates from the pre-operative skin and surgical wound of patients undergoing spinal operations; this study was a large randomized controlled trial investigating the effects of povidone-iodine-alcohol (PVI) and chlorhexidine gluconate-alcohol on surgical wound contamination compared to PVI alone [ 66 ]. The validation of the multiplex method by a number of independent groups has led to the conclusion that it is a robust and valid method for P. acnes typing [ 62 ].…”

Section: Newer Molecular Typing Methodologies For Propiomentioning

confidence: 99%

Over a Decade of recA and tly Gene Sequence Typing of the Skin Bacterium Propionibacterium acnes: What Have We Learnt?

McDowell

2017

Microorganisms

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The Gram-positive, anaerobic bacterium Propionibacterium acnes forms part of the normal microbiota on human skin and mucosal surfaces. While normally associated with skin health, P. acnes is also an opportunistic pathogen linked with a range of human infections and clinical conditions. Over the last decade, our knowledge of the intraspecies phylogenetics and taxonomy of this bacterium has increased tremendously due to the introduction of DNA typing schemes based on single and multiple gene loci, as well as whole genomes. Furthermore, this work has led to the identification of specific lineages associated with skin health and human disease. In this review we will look back at the introduction of DNA sequence typing of P. acnes based on recA and tly loci, and then describe how these methods provided a basic understanding of the population genetic structure of the bacterium, and even helped characterize the grapevine-associated lineage of P. acnes, known as P. acnes type Zappe, which appears to have undergone a host switch from humans-to-plants. Particular limitations of recA and tly sequence typing will also be presented, as well as a detailed discussion of more recent, higher resolution, DNA-based methods to type P. acnes and investigate its evolutionary history in greater detail.

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Antimicrobial Susceptibility, Biotypes and Phylotypes of Clinical Cutibacterium (Formerly Propionibacterium) acnes Strains Isolated from Acne Patients: An Observational Study

Zhang

Yuan

Xin

et al. 2019

Dermatol Ther (Heidelb)

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IntroductionThe aim of this study was to investigate the distribution of antimicrobial susceptibility, biotypes and phylotypes of clinical Cutibacterium acnes (C. acnes, formerly Propionibacterium acnes) isolates as well as the relationship among demographic factors, C. acnes biotypes and phylotypes.MethodsCutibacterium acnes was collected from the skin lesions of acne patients who visited the dermatologic department of Huashan Hospital in Shanghai from October 2016 to March 2017. The agar dilution method was conducted to determine the minimum inhibitory concentrations (MICs) of C. acnes, the fermentation test to identify biotypes and then multiplex touchdown polymerase chain reaction (PCR) to identify phylotypes.ResultsOf the 63 C. acnes strains we isolated, 18 (28.6%), 31 (49.2%) and 4 (6.3%) strains were resistant to clindamycin, erythromycin and moxifloxacin, respectively; no strains were resistant to tetracycline, minocycline, fusidic acid or β-lactam, while metronidazole was completely resisted; 3 strains showed multidrug resistance (MDR). Biotype III (BIII) was the major biotype (50.8%) followed by BI and BV (both 15.9%), BII (12.7%) and lastly BIV (4.8%). IA1 was the predominant phylotype (71.4%) followed by IA2 (19.0%), II (4.8%), IB (3.2%) and IC (1.6%), while III was not detected. Significant differences were observed in the severity of disease: different degrees of acne severity reflected different biotype and phylotype distributions, and the biotype distribution of mild acne was different from that of moderate acne; the phylotype distribution of moderate acne varies from that of severe acne, too. Additionally, there was no significant difference in the distribution of biotypes or phylotypes between resistant and susceptible strains.ConclusionErythromycin and clindamycin resistances are the most common in clinical C. acnes strains; BIII is the predominant biotype and IA1 is the major phylotype of C. acnes, which are mainly related to disease severity.

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Optimization of genotypic and biochemical methods to profile P. acnes isolates from a patient population

Cited by 5 publications

References 34 publications

Control of Propionibacterium acnes by natural antimicrobial substances: Role of the bacteriocin AS-48 and lysozyme

Control of Propionibacterium acnes by natural antimicrobial substances: Role of the bacteriocin AS-48 and lysozyme

Over a Decade of recA and tly Gene Sequence Typing of the Skin Bacterium Propionibacterium acnes: What Have We Learnt?

Antimicrobial Susceptibility, Biotypes and Phylotypes of Clinical Cutibacterium (Formerly Propionibacterium) acnes Strains Isolated from Acne Patients: An Observational Study

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