Optimization of gene editing in cowpea through protoplast transformation and agroinfiltration by targeting the phytoene desaturase gene

Bridgeland, Aya; Biswas, Sudip; Tsakirpaloglou, Nikolaos; Thomson, Michael J.; Septiningsih, Endang M.

doi:10.1371/journal.pone.0283837

Cited by 6 publications

(10 citation statements)

References 41 publications

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“…Despite the low transformation efficiency rates observed in cowpea, genome editing tools, like CRISPR/Cas9, have begun to be implemented in cowpea breeding (using A. tumefaciens and A. rhizogenes as carriers) with encouraging results [ 98 , 99 , 100 , 101 , 102 , 103 , 104 ]. Thus far, the plant material used for CRISPR/Cas9 consisted of cowpea varieties and breeding lines.…”

Section: Application Of New Genomic Technologies In Landraces and Ind...mentioning

confidence: 99%

Crop Landraces and Indigenous Varieties: A Valuable Source of Genes for Plant Breeding

Lazaridi,

Kapazoglou,

Gerakari

et al. 2024

Plants

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Landraces and indigenous varieties comprise valuable sources of crop species diversity. Their utilization in plant breeding may lead to increased yield and enhanced quality traits, as well as resilience to various abiotic and biotic stresses. Recently, new approaches based on the rapid advancement of genomic technologies such as deciphering of pangenomes, multi-omics tools, marker-assisted selection (MAS), genome-wide association studies (GWAS), and CRISPR/Cas9 gene editing greatly facilitated the exploitation of landraces in modern plant breeding. In this paper, we present a comprehensive overview of the implementation of new genomic technologies and highlight their importance in pinpointing the genetic basis of desirable traits in landraces and indigenous varieties of annual, perennial herbaceous, and woody crop species cultivated in the Mediterranean region. The need for further employment of advanced -omic technologies to unravel the full potential of landraces and indigenous varieties underutilized genetic diversity is also indicated. Ultimately, the large amount of genomic data emerging from the investigation of landraces and indigenous varieties reveals their potential as a source of valuable genes and traits for breeding. The role of landraces and indigenous varieties in mitigating the ongoing risks posed by climate change in agriculture and food security is also highlighted.

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Section: Application Of New Genomic Technologies In Landraces and Ind...mentioning

confidence: 99%

Crop Landraces and Indigenous Varieties: A Valuable Source of Genes for Plant Breeding

Lazaridi,

Kapazoglou,

Gerakari

et al. 2024

Plants

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“…Increasing the yield potential and making it stable across environments is a complex challenge that will benefit from adopting new precision breeding techniques like GE [135]. The CRISPR/Cas9 technology provides novel avenues to stimulate functional genomics analyses of various important biotic and abiotic stress tolerance traits in grain legume crops, especially those with efficient protocols for plant transformation and the regeneration of whole plants, like cowpea [138]. There needs to be more mutation resources and efficient means for gene inactivation to adapt GE for cowpea genetic improvements [138].…”

Section: Applications Of Genome Editing In Cowpeamentioning

confidence: 99%

“…The CRISPR/Cas9 technology provides novel avenues to stimulate functional genomics analyses of various important biotic and abiotic stress tolerance traits in grain legume crops, especially those with efficient protocols for plant transformation and the regeneration of whole plants, like cowpea [138]. There needs to be more mutation resources and efficient means for gene inactivation to adapt GE for cowpea genetic improvements [138]. To this end, a couple of studies recently demonstrated the potential to use CRISPR/Cas9mediated GE in cowpea [136,[138][139][140].…”

Section: Applications Of Genome Editing In Cowpeamentioning

confidence: 99%

Enhancing Cowpea Tolerance to Elevated Temperature: Achievements, Challenges and Future Directions

Mohammed,

Ongom,

Togola

et al. 2024

Agronomy

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Despite its ability to thrive in high-temperature environments, cowpea productivity can be hampered by heat stress, particularly when night air temperatures exceed 17 °C. The crop’s germplasm pool potentially possesses significant genetic variability that can be harnessed to breed for heat-tolerant varieties. Progress in improving the crop for heat tolerance has been limited, especially under the hot, short-day environments typical of sub-Saharan Africa. Only a few heat-tolerant varieties have been released, partly due to the limited understanding of heat stress tolerance mechanisms and environmental interaction effects on genotypes, as well as imprecise phenotyping. This review contributes to the literature on cowpea heat stress by highlighting key achievements, challenges, and future directions in breeding heat-tolerant cowpea genotypes and by providing additional information from the recent literature. We opine that the genetic variability for heat tolerance-related traits in cowpea has not been sufficiently exploited in developing varieties adapted to the target production environments. Therefore, attention should be given to assessing the crop’s genetic repository by targeting adaptive, morphological, and physiological traits that enhance heat stress tolerance. We propose that breeding programs integrate phenotyping of whole-plant physiological traits and molecular breeding to identify breeder-friendly markers for routine selection. This should be followed by introgression of the heat-tolerant favourable alleles to adapted susceptible varieties using rapid and precise approaches that take advantage of modern genetic and genomic resources such as innovative genetic resources, genomic selection, speed breeding, and genome editing technologies. These tools hold great promise in fast-tracking the development of improved heat-tolerant varieties and incorporating the must-have traits preferred by cowpea farmers and consumers. In view of the likely increase in atmospheric temperature to be occasioned by climate change, there is an urgent need to develop heat-tolerant cowpea varieties to ensure the sustainability of current and future cropping and agri-food systems.

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“…This refers to the ability of a genetically transformed plant cell or tissue to regenerate into a full plant that expresses and propagates the introduced traits. Despite the mentioned difficulties in applying genome editing to legume crops, example of successful transformations include soybean ( Lu and Tian, 2022 ), Lotus japonicus ( Wang et al., 2019 ) , Medicago truncatula ( Jaudal et al., 2022 ) , cowpea ( Bridgeland et al., 2023 ), peanut ( Yuan et al., 2019 ), pea ( Li et al., 2023 ), and chickpea ( Gupta et al., 2023 ). To date, no CRISPR/Cas9 studies have been reported for faba bean ( Bhowmik et al., 2021 ).…”

Section: Prospects For the Development Of Faba Bean Ideotypes With Ne...mentioning

confidence: 99%

The challenge of breeding for reduced off-flavor in faba bean ingredients

Lippolis,

Roland,

Bocova

et al. 2023

Front. Plant Sci.

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The growing interest in plant protein sources, such as pulses, is driven by the necessity for sustainable food production and climate change mitigation strategies. Faba bean (Vicia faba L.) is a promising protein crop for temperate climates, owing to its remarkable yield potential (up to 8 tonnes ha−1 in favourable growing conditions) and high protein content (~29% dry matter basis). Nevertheless, the adoption of faba bean protein in plant-based products that aim to resemble animal-derived counterparts is hindered by its distinctive taste and aroma, regarded as “off-flavors”. In this review, we propose to introduce off-flavor as a trait in breeding programs by identifying molecules involved in sensory perception and defining key breeding targets. We discuss the role of lipid oxidation in producing volatile and non-volatile compounds responsible for the beany aroma and bitter taste, respectively. We further investigate the contribution of saponin, tannin, and other polyphenols to bitterness and astringency. To develop faba bean varieties with diminished off-flavors, we suggest targeting genes to reduce lipid oxidation, such as lipoxygenases (lox) and fatty acid desaturases (fad), and genes involved in phenylpropanoid and saponin biosynthesis, such as zero-tannin (zt), chalcone isomerase (chi), chalcone synthase (chs), β-amyrin (bas1). Additionally, we address potential challenges, including the need for high-throughput phenotyping and possible limitations that could arise during the genetic improvement process. The breeding approach can facilitate the use of faba bean protein in plant-based food such as meat and dairy analogues more extensively, fostering a transition toward more sustainable and climate-resilient diets.

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Optimization of gene editing in cowpea through protoplast transformation and agroinfiltration by targeting the phytoene desaturase gene

Cited by 6 publications

References 41 publications

Crop Landraces and Indigenous Varieties: A Valuable Source of Genes for Plant Breeding

Crop Landraces and Indigenous Varieties: A Valuable Source of Genes for Plant Breeding

Enhancing Cowpea Tolerance to Elevated Temperature: Achievements, Challenges and Future Directions

The challenge of breeding for reduced off-flavor in faba bean ingredients

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