Optimization of Fungal Dextranase Production and Its Antibiofilm Activity, Encapsulation and Stability in Toothpaste

Juntarachot, Nucharee; Kantachote, Duangporn; Peerajan, Sartjin; Sirilun, Sasithorn; Chaiyasut, Chaiyavat

doi:10.3390/molecules25204784

Cited by 16 publications

(17 citation statements)

References 32 publications

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“…Others have found similar findings, e.g., microencapsulation of S. boulardii in Ch-coated Alg beads. 36 , 37 A study by Juntarachot et al 38 showed that encapsulation of dextranase in Alg beads increased the stability for up to 3 months without any significant change in their activity. Koo et al 20 speculated that coating of Alg beads with Ch improved the chemical and mechanical strength of the beads and allowed for longer-term storage.…”

Section: Resultsmentioning

confidence: 99%

Microencapsulation of Probiotic Streptococcus salivarius LAB813

et al. 2023

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Probiotics are living microorganisms that confer a health benefit on the host when administered in adequate amounts. Streptococcus salivarius, a commensal bacterium found in the oral cavity, has been shown to secrete antimicrobial peptides and can be used as probiotics. This study aimed to develop a delivery system for the probiotic LAB813, a novel S. salivarius strain first identified in the laboratory. Probiotics can be delivered and protected through the encapsulation of biomaterials such as polysaccharides. Their biocompatibility, biodegradability, user-friendliness, and ease of access make polysaccharides useful for encapsulating probiotics. Alginate (Alg) and chitosan (Ch) are naturally obtained polysaccharides and, hence, tested for LAB813 encapsulation. An extrusion method of encapsulation was performed to form Alg microcapsules (Alg-LAB813), some of which were coated with Ch (Alg-LAB813-Ch) to provide dual-layered protection. Inhibitory assays of the Alg-LAB813 and Alg-LAB813-Ch microcapsules were assayed against an indicator strain. Alg-LAB813-Ch microcapsules showed superior antibacterial properties compared to Alg-LAB813 microcapsules over 24 h and when subject to temperatures ranging from 4 to 68 °C. In addition, Alg-LAB813-Ch microcapsules retained antibacterial activity for up to 28 days of storage at 4 °C. The strong and sustained inhibitory activities of Ch-coated Alg encapsulated LAB813 signify the potential for their use to improve oral health.

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Section: Resultsmentioning

confidence: 99%

Microencapsulation of Probiotic Streptococcus salivarius LAB813

et al. 2023

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“…In sugar industry, dextranase can remove dextran to improve production efficiency, yield, and quality of sucrose (Bhatia et al, 2010(Bhatia et al, , 2016Eggleston et al, 2009;Sufiate et al, 2018;Zhao et al, 2021). Dextranase has many applications in the prevention and treatment of dental caries (Jiao et al, 2014;Juntarachot et al, 2020;Lai et al, 2019;Ren et al, 2016). High molecular dextran can be hydrolyzed by dextranase to prepare low molecular weight compounds or isomaltooligosaccharides (IGs).…”

Section: Dextranasementioning

confidence: 99%

Research progress on structure and catalytic mechanism of dextranase

Xia

et al. 2023

eFood

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Dextranase is an important industrial enzyme for the preparation of isomaltooligosaccharides. According to the crystal structures of 20 dextranases of four classes, we summarized the structural characteristics of enzymes and the binding modes of enzymes and ligands in this paper. Based on the characteristics of the products, we analyzed the characteristics of the binding domain and functions of dextranase by means of molecular simulation and molecular docking. The relationship between the product specificity of dextranase, its catalytic pocket shape, and its catalytic mode was briefly summarized. The catalytic mechanism of dextranase was systematically discussed, which provided the basis and reference for the rational transformation of dextranase.

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“…There are numerous causes of dextranase instability in oral care products, including temperature, acidity, alkalinity, surfactants, and other oral care product components [ 92 ]. Fungal dextranase is widely used in the food industry as it is safe and easy to obtain after harvesting mushrooms [ 93 ].…”

Section: Comparison Of Dextranases From Marine Bacteria With Those Fr...mentioning

confidence: 99%

Marine Bacterial Dextranases: Fundamentals and Applications

et al. 2022

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Dextran, a renewable hydrophilic polysaccharide, is nontoxic, highly stable but intrinsically biodegradable. The α-1, 6 glycosidic bonds in dextran are attacked by dextranase (E.C. 3.2.1.11) which is an inducible enzyme. Dextranase finds many applications such as, in sugar industry, in the production of human plasma substitutes, and for the treatment and prevention of dental plaque. Currently, dextranases are obtained from terrestrial fungi which have longer duration for production but not very tolerant to environmental conditions and have safety concerns. Marine bacteria have been proposed as an alternative source of these enzymes and can provide prospects to overcome these issues. Indeed, marine bacterial dextranases are reportedly more effective and suitable for dental caries prevention and treatment. Here, we focused on properties of dextran, properties of dextran—hydrolyzing enzymes, particularly from marine sources and the biochemical features of these enzymes. Lastly the potential use of these marine bacterial dextranase to remove dental plaque has been discussed. The review covers dextranase-producing bacteria isolated from shrimp, fish, algae, sea slit, and sea water, as well as from macro- and micro fungi and other microorganisms. It is common knowledge that dextranase is used in the sugar industry; produced as a result of hydrolysis by dextranase and have prebiotic properties which influence the consistency and texture of food products. In medicine, dextranases are used to make blood substitutes. In addition, dextranase is used to produce low molecular weight dextran and cytotoxic dextran. Furthermore, dextranase is used to enhance antibiotic activity in endocarditis. It has been established that dextranase from marine bacteria is the most preferable for removing plaque, as it has a high enzymatic activity. This study lays the groundwork for the future design and development of different oral care products, based on enzymes derived from marine bacteria.

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Optimization of Fungal Dextranase Production and Its Antibiofilm Activity, Encapsulation and Stability in Toothpaste

Cited by 16 publications

References 32 publications

Microencapsulation of Probiotic Streptococcus salivarius LAB813

Microencapsulation of Probiotic Streptococcus salivarius LAB813

Research progress on structure and catalytic mechanism of dextranase

Marine Bacterial Dextranases: Fundamentals and Applications

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