Optimization of formulation processes using Design Expert ® Software for preparation of polymeric blends-artesunate-amodiaquine HCl microparticles

Optimization of a new artemisinin-based combination therapy of artesunate-piperaquine fixed-dose combination tablet with enhanced in vivo antimalarial effects

“…The percentage of infected erythrocytes was determined in blood films fixed with methanol and stained with Giemsa. Parasites were counted per 10,000 RBCs per slide, minimum of 20 fields (500 RBCs for each field) were counted in one slide, and average parasitemia was measured (Osmo et al, 1993). Parasitemia levels and survival rate were monitored and calculated up to 28 days after infection.…”

Formulation, In vitro and In vivo Evaluation of Sustained released Artemether-Lumefantrine-Loaded microstructured solid Lipid Microparticles (SLMs)

“…Loading capacity (LC) expresses the ratio of the entrapped active pharmaceutical ingredient (API) to the total weight of the lipids. 22 The LC of the SLMs was determined using the formula;…”

“…The determination of encapsulation efficiency (EE) was done according to Ogbonna et al with little modifications. 22 A 200 mg each of the lyophilized SLMs batches AL1, AL2 and AL3 containing 3%, 5% and 7% of AL respectively was crushed and soaked in 10 mL methanol for 24 h. Thereafter, the following procedures were carried out. A 0.5 mL of the filtrate in each batch was diluted to 5 mL using methanol.…”

“…The amount of drug released at each time interval was determined using the standard Beer's plot for lumefantrine. According to Ogbonna et al; 22 Niesko et al 24 with little modification, the artemether was derivatized and the drug released determined. The same procedure was repeated using SIF and Tween 80.…”

Hot melt extruded polysaccharide blends for controlled drug delivery

“…Polymer blends offer an interesting potential for controlled drug delivery systems [1,2,3,4,5,6], both as matrix formers [7,8,9,10] and as coating materials [11,12,13,14,15]. By simply varying the polymer:polymer blend ratio, the resulting key properties of the systems can be effectively varied, allowing to provide large spectra of possible drug release kinetics [16,17,18].…”