Optimization of fibrolytic enzyme production by Aspergillus japonicus C03 with potential application in ruminant feed and their effects on tropical forages hydrolysis

Abstract

Fibrolytic enzyme production by Aspergillus japonicus C03 was optimized in a medium containing agro-industrial wastes, supplemented with peptone and yeast extract. A 2(3) full factorial composite and response surface methodology were used to design the experiments and analysis of results. Tropical forages were hydrolyzed by A. japonicus C03 enzymatic extract in different levels, and they were also tested as enzymatic substrate. Optimal production to xylanase was obtained with soybean bran added to crushed corncob (1:3), 0.01% peptone, and 0.2% yeast extract, initial pH 5.0, at 30 °C under static conditions for 5 days of incubation. Optimal endoglucanase production was obtained with wheat bran added to sugarcane bagasse (3:1), 0.01% peptone, and 0.2% yeast extract, initial pH 4.0, at 30 °C, for 6 days, under static conditions. Addition of nitrogen sources as ammonium salts either inhibited or did not influence xylanase production. This enzymatic extract had a good result on tropical forage hydrolyzes and showed better performance in the Brachiaria genera, due to their low cell wall lignin quantity. These results represent a step forward toward the use of low-cost agricultural residues for the production of valuable enzymes with potential application in animal feed, using fermentation conditions.

Effect of Mediterranean saltbush (Atriplex halimus) ensilaging with two developed enzyme cocktails on feed intake, nutrient digestibility and ruminal fermentation in sheep

“…This may be due to reduction of structural polysaccharide fractions (Facchini et al . ). The difference in response between ZAD1 ® and ZAD2 ® or their combination depends on their different enzyme contents (Table ).…”

Feed intake, nutrient digestibility, nitrogen utilization, and ruminal fermentation activities in sheep fed Atriplex halimus ensiled with three developed enzyme cocktails

“…with Z enzyme at 4 g/animal/day, D3 = Z1 ensiled AH supplemented with Z enzyme at 4 g/animal/day, D4 = Z2 ensiled AH supplemented with Z enzyme at 4 g/animal/day, D5 = Z1 + Z2 ensiled AH supplemented with Z enzyme at 4 g/animal/day 1 all diets supplemented with 300 g air-dried barley/animal/day or 266 g dry matter/animal/day a-d means in the same row with different superscripts are significantly different (P < 0.05) of structural polysaccharide fractions (Facchini et al 2011). The different response between Z1 and Z2 or their combination depends on the content of different enzymes (Table 1).…”

Lipolytic Potential ofAspergillus japonicusLAB01: Production, Partial Purification, and Characterisation of an Extracellular Lipase

“…Aspergillus japonicus is amenable for biotechnological analysis due to its phytase [1], cellulose [2], pectinase [3], xylanase [4, 5], and beta-fructofuranosidase [6] production capacity. Furthermore, the production of fibrolytic enzymes is well described; however, their lipolytic potential remains unknown.…”