Optimization of Fermentation Parameters to Enhance the Production of Ethanol from Palmyra Jaggery Using Saccharomyces cerevisiae in a Batch Fermentor

Abstract

Application of statistical experimental designs for optimization of fermentation parameters to enhance ethanol production, which is an economical and renewable energy source using Saccharomyces cerevisiae NCIM 3090 from palmyra jaggery, was studied in a batch fermentor. Using Plackett-Burman design, impeller speed, concentrations of CoCl2 and KH2PO4 were identified as significant variables, which highly influenced ethanol production, and these variables were further optimized using a central composite design (CCD). The ethanol production was adequately approximated with a full quadratic equation obtained from three factors and five levels of CCD. Maximum ethanol concentration of 132.56 g/l (16.8% [v/v]) was obtained for an impeller speed of 247.179 ( approximately 250) rev/min, CoCl2 of 0.263 g/l and KH2PO4 of 2.39 g/l. A second-order polynomial regression model was fitted and was found adequate with R 2 of 0.8952. This combined statistical approach enables rapid identification and investigation of significant parameters for improving the ethanol production and could be very useful in optimizing processes.

Ethanol production from cashew apple juice using statistical designs

“…Among them, response surface methodology (RSM) using a central composite design (CCD) is a suitable method for identifying the effect of individual variables and optimum conditions for a multivariable system efficiently. CCD was used previously for optimization of fermentation conditions/parameters for the production of ethanol from sago starch [6], from Palmyra jaggery [7], finger millet medium [8,9], rapeseed straw [10] and optimization of medium constituents for the production of citric acid from pineapple waste [11].…”

Simultaneous Saccharification of Corn Starch in Gluconic Acid Production by Aspergillus niger Immobilized on Nonwoven Fabric in a Pressurized Reactor

“…Immobilization of whole cells or glucose oxidase enzyme by various techniques has also been reported to be a useful approach for the production of GA. Nonwoven fabric has been used as a support for immobilizing proteins [8] or whole cells [9] since it is porous, has a large surface area, and can be easily processed. In addition, it is feasible to use cheap raw materials such as cellulose, molasses, starch and whey for the fermentation, and simultaneous saccharification of such materials for the fermentation (SSF) has been used in an efficient process with ethanol [10,11], lactic acid [12,13], etc. Since SSF for GA production from starch is considered to be comprised of starch solubilization by heating, followed by the liquefaction/saccharification of starch and GA production, thermostable or thermophilic amylase aided liquefaction might be useful to avoid the gelatinization of the solubilized starch.…”