Optimization of Fermentation Parameters for β-Glucosidase Production by Aspergillus niger

Optimisation of soy flour fermentation parameters to produce β-glucosidase for bioconversion into aglycones

“…Therefore, to achieve maximum b-glucosidase production by the SSF of DSF with A. oryzae IOC 3999/1998, the recommendations are to use 10 mL of water added to 10 g of DSF (X 2 ), with an incubation temperature of 30°C (X 3 ) and a pH (X 1 ) between 5.2 and 6.8. Qian et al (2012) found that the maximum b-glucosidase production in an SSF of wheat bran with A niger occurred at a pH 6.0, with 70% moisture for 72 h incubation at 28°C, whereas lower b-glucosidase activity was observed between 32 and 36°C. However, when using SSF on sorghum bagasse with the same fungus, conditions including a pH of 5.0, 70% moisture and 96 h incubation at 30°C produced maximum b-glucosidase activity (Lavudi, Harinder, & Laximi, 2003).…”

“…The culture media should be favourable for growth and contain all the nutrients necessary for cell synthesis and desired product formation (Ward, 1991). The production of fungal b-glucosidase by SSF depends on the pH and initial substrate moisture, temperature and time of incubation (Qian, Fu, Zhou, Sun, & Weng, 2012). Therefore, to increase the SSF process yield, it is important to optimise the physical and chemical parameters (Francis et al, 2003).…”

Canola meal as a novel substrate for β-glucosidase production by Trichoderma viride: application of the crude extract to biomass saccharification

“…Despite fungal b-glucosidases have been produced using other lignocellulosic wastes such as rice husk [32] and wheat bran [33], canola meal has not been exploited as substrate for microbial growth and b-glucosidase production. Table 2 shows the ANOVA for b-glucosidase production.…”

Produção e caracterização bioquímica de enzimas lignocelulolíticas fúngicas e sua aplicação na sacarificação de biomassa lignocelulósica

“…Embora muitas pesquisas sejam conduzidas utilizando o cultivo submerso, o qual permite melhor controle das condições de aeração, pH e temperatura, o cultivo em meio sólido tem sido preferido para a produção de enzimas lignocelulolíticas (Kaur et al, 2006;Yang et al, 2008;Sonia et al, 2008;Liang et al, 2011;Qian et al, 2012;Delabona et al, 2012 (Qian et al, 2012;Masui et al, 2011;Zimbardi et al, 2012). Isto pode ser atribuído à sua composição nutricional, pois este resíduo apresenta cerca de 14% de proteínas, 27%…”

“…niger apresentou uma produção de 508,0 U g -1 , quando cultivado em farelo de trigo suplementado com sais minerias (Qian et al, 2012 Os valores de pH e temperatura encontrados para a maioria das β-glucosidases de fungos mesófilos estão na faixa de 4,0 a 6,5 e 50 a 65°C, respectivamente (Sorensen et al, 2013). No entanto, uma β-glucosidase produzida por Fomitopsis palustres apresentou temperatura ótima de 70°C, enquanto as β-glucosidases de P. brasilianum, A. niger (N188) e Phanerochaete chrysosporium apresentaram temperatura ótima de apenas 22°C (Sorensen et al, 2013;Teugjas & Väljamäe, 2013).…”