Optimization of Fermentation Conditions for Recombinant Human Interferon Beta Production by Escherichia coli Using the Response Surface Methodology

Morowvat, Mohammad Hossein; Babaeipour, Valiollah; Memari, Hamid Rajabi; Vahidi, Hossein

doi:10.5812/jjm.8(4)2015.16236

Cited by 37 publications

(21 citation statements)

References 35 publications

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“…13 Culture medium determines the nutritional and chemical environment of microorganisms during cultivation. 14 Hence, investigation regarding the physiologic and metabolic changes in the microalgal cells, their growth pattern and also their composition during nutrient limitation in culture medium is of importance. The obtained data could be employed to increase the growth rates and also improve the β-carotene production process.…”

Section: Introductionmentioning

confidence: 99%

Effects of Sulfur, Iron and Manganese Starvation on Growth, β-carotene Production and Lipid Profile of Dunaliella salina

Shaker¹,

Morowvat²,

Ghasemi³

2017

JYP

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Objective: Microalgal pharmaceutical biotechnology is mainly dependent on the biomass yield and also the final concentration of the obtained lipids. β-carotene is one of the most precious nutraceuticals, of both preventive and therapeutics importance in pharmacy and medicine. Dunaliella salina is known as famous β-carotene producer which could accumulate the β-carotene up to 10% of its dry cell weights. The amount of different macro and micronutrients in D. salina culture medium defines its productivity and β-carotene content. Methods: In this study, the effects of sulfur, iron and manganese deprivation, on cell growth and β-carotene biosynthesis in a naturally isolated strain of D. salina was examined. Besides, the fatty acid profile of the naturally isolated strain was also investigated. Results: Sulfur, iron and manganese deprivation caused a noticeable decrease in the cell growth of D. salina. On the other hand, in nutrient depleted media, the maximum β-carotene concentration was significantly improved (14.616 mg L-1 in sulfur starvation, 14.994 mg L-1 in iron starvation and 10.119 mg L-1 in manganese starvation media) compared with initial values (6.753 mg L-1) in basic culture medium. The obtained fatty acids from the studied microalgal strain found to be some important saturated, monounsaturated and polyunsaturated fatty acids. Conclusion: Owing to its significant growth rate, β-carotene contents and fatty acid profile; the naturally isolated microalgal strain could be exploited as a potential producer strain. Besides, the nutrient limitation strategy could be effectively employed to improve the β-carotene production procedure in D. salina. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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Section: Introductionmentioning

confidence: 99%

Effects of Sulfur, Iron and Manganese Starvation on Growth, β-carotene Production and Lipid Profile of Dunaliella salina

Shaker¹,

Morowvat²,

Ghasemi³

2017

JYP

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“…The recombinant human interferon beta (rhIFN-β)-1b was optimized in shake flasks using response surface methodology (RSM). The production of rhIFN-β-1b was significantly increased using the optimized conditions, and the rhIFN-β production was increased from 0.255 g·L −1 to 0.981 g·L −1 [ 14 ]. In our present study, we use this approach to improve the fermentation conditions of CS5931, and the results show that the production of CS5931 is greatly increased; the yield of the purified polypeptide was increased from 2.0 mg/L to 7.5 mg/L.…”

Section: Discussionmentioning

confidence: 99%

Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi

Chen

Li³

et al. 2016

Marine Drugs

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CS5931 is a novel anticancer agent isolated from the sea squirt Ciona savignyi. However, its content in the species is very low, and developing a novel approach for production of the polypeptide is promising. In the present study, we expressed and purified the polypeptide from E. coli, and the fermentation conditions were studied using response surface methodology. The yield of CS5931 was increased from 2.0 to 7.5 mg/L. The denaturing and renaturation conditions were also studied. Using the optimized renaturation condition, the anticancer activity of refolding CS5931 was increased significantly; the value of IC50 was decreased from 23.2 to 11.6 μM. In vivo study using xenograft nude mice bearing HCT116 cancer cells revealed that CS5931 was able to inhibit the growth of tumor significantly. The study provides a useful approach for obtaining enough amount of CS5931 for further study. This study is also important for developing the polypeptide as a novel anticancer agent.

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“…Our previous study indicated that glycoprotein GP-1 production was strongly affected by medium components, especially the types and concentrations of carbon and nitrogen sources [ 14 ]. Process factors (temperature, pH, rotating speed) also play an important role in improving objective metabolites production and may contribute to the reduction of fermentation cost, even changing the path of microbial biosynthesis to discover new natural products [ 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Fermentation Conditions and Bench-Scale for Improvement of a Novel Glycoprotein GP-1 Production by Streptomyces kanasenisi ZX01

Zhou

et al. 2018

Molecules

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GP-1 is a novel glycoprotein produced by Streptomyces kanasenisi ZX01 that was isolated from soil near Kanas Lake with significant bioactivity against tobacco mosaic virus. However, extremely low fermentation production has largely hindered further research and market applications on glycoprotein GP-1. In this study, response surface methodology was used to optimize fermentation conditions in a shake flask for higher glycoprotein GP-1 production. When the optimized fermentation conditions were inoculum volume of 6%, initial pH of 6.5, and rotating speed of 150 rpm, glycoprotein GP-1 production could reach 0.9253 mg/L, which was increased by 52.14% compared to the original conditions. In addition, scale-up fermentation was conducted in a 5-L bioreactor to preliminarily explore the feasibility for mass production of glycoprotein GP-1 in a large fermentor, obtaining GP-1 production of 2.54 mg/L under the same conditions, which was 2.75 times higher than the production obtained from a shake flask of 0.9253 mg/L. This work will be helpful to improve GP-1 production on a large scale and lay the foundations for developing it as a novel agent against plant virus.

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Optimization of Fermentation Conditions for Recombinant Human Interferon Beta Production by Escherichia coli Using the Response Surface Methodology

Cited by 37 publications

References 35 publications

Effects of Sulfur, Iron and Manganese Starvation on Growth, β-carotene Production and Lipid Profile of Dunaliella salina

Effects of Sulfur, Iron and Manganese Starvation on Growth, β-carotene Production and Lipid Profile of Dunaliella salina

Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi

Optimization of Fermentation Conditions and Bench-Scale for Improvement of a Novel Glycoprotein GP-1 Production by Streptomyces kanasenisi ZX01

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