Optimization of Fabrication Parameters To Produce Chitosan−Tripolyphosphate Nanoparticles for Delivery of Tea Catechins

Abstract

This work investigated the polyanion-initiated gelation process in fabricating chitosan-tripolyphosphate (CS-TPP) nanoparticles intended to be used as carriers for delivering tea catechins. The results demonstrated that the particle size and surface charge of CS-TPP nanoparticles could be controlled by fabrication conditions. For preparation of CS-TPP nanoparticles loaded with tea catechins, the effects of modulating conditions including contact time between CS and tea catechins, CS molecular mass, CS concentration, CS-TPP mass ratio, initial pH value of CS solution, and concentration of tea catechins on encapsulation efficiency and the release profile of tea catechins in vitro were examined systematically. The study found that the encapsulation efficiency of tea catechins in CS-TPP nanoparticles ranged from 24 to 53%. In addition, FT-IR analysis showed that the covalent bonding and hydrogen bonding between tea catechins and CS occurred during the formation of CS-TPP nanoparticles loaded with tea catechins. Furthermore, studies on the release profile of tea catechins in vitro demonstrated that the controlled release of tea catechins using CS-TPP nanoparticles was achievable.

Chitosan–tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and antiulcerogenic activity

“…A similar phenomenon was observed by Hu et al 37 where aggregates with a large diameter were formed when the CS-TPP mass ratio was decreased to 1.5. The optimized CS-TPP mass ratio was reported as 5 37 and 3.75.…”

“…A similar phenomenon was observed by Hu et al 37 where aggregates with a large diameter were formed when the CS-TPP mass ratio was decreased to 1.5. The optimized CS-TPP mass ratio was reported as 5 37 and 3.75. 35 Our studies indicated that CS concentrations of 1 mg⋅mL -1 , with a CS-TPP polymer ratio (w:w) of 5, a CS-TPP volume ratio of 10, and using mechanical stirring at 7,200 rpm, followed by sonication (5 minutes, 20% amplitude) resulted in the reproducible formation of NPs with an average hydrodynamic diameter of 150±13 nm and a ZP of +45±2 mV, with high yields (75%).…”

Characterization and anticancer potential of ferulic acid-loaded chitosan nanoparticles against ME-180 human cervical cancer cell lines

“…Attempts have been made to increase its solubility and bioavailability through encapsulation in biodegradable polymeric nanoparticles (Vemula et al 2006;Salmaso et al 2007). Chitosan (CS), the deacetylated form of chitin (2-amino-2-deoxy-(1-4)-Dglucopyranan), exhibits excellent biodegradability, biocompatibility and antimicrobial activity (Liu et al 2006); thus, CS nanoparticles are extensively used to deliver hydrophobic compounds including drugs, vitamins, proteins, nutrients and phenolics into the biological systems (Hu et al 2008;Jang and Lee 2008). CS acts as a wall/shell material to envelop these compounds bearing multiple negative charges via cationic crosslinking to generate stable, non-toxic, biodegradable nanosized particles (Keawchaoon and Yoksan 2001).…”

Preparation, characterization and rheological behavior of chitosan nanocapsule emulsion encapsulated tuberose fragrance

“…Afterward, the particle size increased as the pH value rose from 4.0 to 5.5. The comparatively weak interaction between CS and TPP led to the formation of larger nanoparticles 26 . However, the heterogeneity became higher and higher as pH increased, which may also be related to the CS solubility.…”