Optimization of extraction procedure and chromatographic separation of glyphosate, glufosinate and aminomethylphosphonic acid in soil

Druart, Céline; Delhomme, Olivier; Vaufleury, Annette de; Ntcho, Evodie; Millet, Maurice

doi:10.1007/s00216-010-4468-z

Cited by 62 publications

(66 citation statements)

References 19 publications

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“…Aqueous samples were removed and filtered (0.2 μm), and the total aqueous radioactivity was estimated using LSS. The soil was extracted with 20 mL of NaOH (0.1 mol L −1 ) in a Teflon centrifuge tube with horizontal shaking following the method described by Druart et al (2011). Extracts were centrifuged at 12,000 rpm for 15 min, an aliquot was removed for LSS (to quantify extractable radioactivity), and the supernatant was retained for analysis of the herbicide.…”

Section: Methodsmentioning

confidence: 99%

Effect of Soil Aeration and Phosphate Addition on the Microbial Bioavailability of Carbon-14-Glyphosate

2015

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The adsorption, desorption, degradation, and mineralization of C-glyphosate [-(phosphonomethyl)glycine] were examined in Catlin (a fine-silty, mixed, superactive, mesic Oxyaquic Argiudoll), Flanagan (a fine, smectitic, mesic Aquic Argiudoll), and Drummer (a fine-silty, mixed, superactive, mesic Typic Endoaquoll) soils under oxic and anoxic soil conditions. With the exception of the Drummer soil, soil aeration did not significantly alter the adsorption pattern of C-glyphosate to soils. Herbicide desorption was generally enhanced with anaerobiosis in all the soil types. Anoxic soils demonstrated slower microbial degradation and mineralization kinetics of C-glyphosate than oxic soils in all the soil types studied. Phosphate additions significantly reduced the adsorption of C-glyphosate to soils irrespective of soil aeration and confirmed the well-established competitive adsorption theory. The addition of soil phosphate stimulated degradation only in anoxic soils. The results from this research highlight the importance of soil redox conditions as an important factor affecting the bioavailability and mobility of glyphosate in soils.

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Section: Methodsmentioning

confidence: 99%

Effect of Soil Aeration and Phosphate Addition on the Microbial Bioavailability of Carbon-14-Glyphosate

2015

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“…Total concentrations of glyphosate, glufosinate, and AMPA are given in milligrams per kilogram of dry weight (dw). Quantification limits, detection limits, and recoveries were 0.17 mg kg −1 , 0.10 mg kg −1 , and 97±17% for glyphosate; 0.03 mg kg −1 , 0.02 mg kg −1 , and 96±8% for glufosinate; and 0.03 mg kg −1 , 0.02 mg kg −1 , and 90±12% for AMPA (Druart et al 2011).…”

Section: Observation Of the Genital Apparatus And Histologymentioning

confidence: 99%

“…Observations and photography were performed on a Zeiss AXIO Imager M2 microscope equipped with a Zeiss AxioCam MRc5 camera. 2.7 Herbicide residue determination in soil, food, and snails Extraction and analysis of soil samples were performed according to Druart et al (2011): briefly, samples of 5 g of soil were defrosted, dried, and extracted (in duplicates for soil without snails) by suspension in water. Soil extracts were analyzed by pre-column derivatization by 9-fluorenylmethyl chloroformate (FMOC-Cl) coupled with high-performance liquid chromatography with fluorescence detection (HPLC-FD).…”

Section: Observation Of the Genital Apparatus And Histologymentioning

confidence: 99%

Glyphosate and glufosinate-based herbicides: fate in soil, transfer to, and effects on land snails

et al. 2011

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International audiencePurpose The aim of this work was to assess the transfer and effects of two widely used herbicides on the land snail Helix aspersa during long-term exposure under laboratory conditions. Materials and methods Newly hatched snails were exposed for 168 days to soil and/or food contaminated with a formulation of glyphosate (BypassÂ®) or glufosinate (BastaÂ®) at the recommended field doses and also at 10-fold this dose. Results and discussion Herbicide degradation patterns showed that snails were mainly exposed during the first 28 days. The DT50 of glyphosate and glufosinate was established at 10.6 and 3.7 days, respectively. No significant effects on survival and growth were determined. Concerning genital tract maturation of the snails, exposure to herbicides tended to decrease the development of the albumen gland (inhibition of 43.5 Â± 32.8%). The presence of glyphosate (6 mg kg âˆ'1 dry weight) was demonstrated in snails exposed continuously to this active ingredient at the highest concentration in their food. Conclusions These results showed a low effect of herbicides at relevant concentrations in soil but the detection of residues in tissues indicated a potential risk of transfer to the food chain. This chronic toxicity bioassay could complete the available tests to assess toxicity of contaminants, and more particularly pesticides, in soil

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“…Examples of glyphosate adduct determination from soil (using ultrasonic water to extract), followed by precolumn derivatization with FMOC in a borate buffer (25 C/1 h/stirring), and followed by LLE (with ethyl ether) 157 or the selection and extraction of acidic herbicides in soil by PFBBr with Na 4 EDTA as a complexing agent for GC/MS analysis are typical. In addition, derivatization has been used to impart stability to keep 'intact' insecticides, triazines, ureas, chlorophenoxy acids, and fungicides for MS identification purposes.…”

Section: Pesticidesmentioning

confidence: 99%

Analytical Derivatization Techniques ☆

Parkinson¹

2014

Reference Module in Chemistry, Molecular Sciences and Chemical Engineering

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For the last 40 years or so, analytical chemists have borrowed well-known reaction techniques from organic and organometallic fields to perform derivatization reactions so as to enhance detection of the elusive analytes they seek. The derivatization mantra has been that, for the technique to be worthwhile, it should be simple to perform, fast (usually less than 15 min), selective, and that the derivatizing reagent should react quantitatively with the analyte and only give one final product. So where are we now? Some derivatization reactions are indeed fast and some are not; some are very selective and some are more universal. However, with a certain care and careful control of optimized conditions, quantitative enhancement outcomes and increased separative power and detection can be very worthwhile. The number of derivatization reagents commercially available itself suggests that there must be some merit to derivatization, that overcomes the associated extra costs: in time (to incorporate the extra reaction step), and in the expense of acquiring, handling, and storing derivatizing reagents. This chapter attempts to show that with proper consideration, it has been feasible to push a chosen analytical technique to give increased selective detection and signal enhancement by derivatization. In many cases, this has made possible the detection of trace components from difficult matrices in a number of scenarios, including on-site analysis, in quality control/continuous analysis methods, or through some remote-sensing platforms. Further, the incorporation of derivatization in an extraction method can be simple and friendly to automated throughput for multiple sample analysis.The fundamental idea of derivatization is that the basic chemical or physical structure of the analytical moiety is changed through a suitable reaction. For example, by labeling compounds for a specific detection purpose, or by changing a functional group to enhance certain chromatographic characteristics, better detection and separation (respectively) can be achieved via the ensuing instrumental techniques. The type of derivatization is highly dependent upon the analytical method and upon the nature of the compound analyzed. There are a variety of good review articles, technical reports, 1 text chapters, 2 and full texts 3-6 that address in detail many specific applications used in analytical derivatization. There are several extraction methods available that are compatible with derivatization. For many analyses, the choice of the best extraction technique is not clear, as many techniques can extract analytes equally well, based on simplicity, applicability, solvent use, expense, detection limit, precision, and time taken to perform the operation. 7 Derivatization techniques have been incorporated into many sample preparation and extractions schemes, to include, but not limited to, total sample headspace, single-drop microextraction (SDME), 8 solid-phase microextraction (SPME), needle trap device (NTD), purge and trap, solid-phase extraction (SPE)...

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Optimization of extraction procedure and chromatographic separation of glyphosate, glufosinate and aminomethylphosphonic acid in soil

Cited by 62 publications

References 19 publications

Effect of Soil Aeration and Phosphate Addition on the Microbial Bioavailability of Carbon-14-Glyphosate

Effect of Soil Aeration and Phosphate Addition on the Microbial Bioavailability of Carbon-14-Glyphosate

Glyphosate and glufosinate-based herbicides: fate in soil, transfer to, and effects on land snails

Analytical Derivatization Techniques ☆

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