Optimization of extracellular keratinase production by poultry farm isolate Scopulariopsis brevicaulis

Anbu, Periasamy; Gopinath, Subash C.B.; Amanda, Hilda; Lakshmipriya, Thangavel; Annadurai, G.

doi:10.1016/j.biortech.2006.05.047

Cited by 80 publications

(61 citation statements)

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“…Hence, this is the pioneering report emphasizing the production of Keratinase at pH 7.5. Further, the significant reduction in keratinase production might be due to non-availability of nutrients due to pH shift or complete depletion of nutrients or feedback inhibition as correlated with the findings [42]. …”

supporting

confidence: 61%

Bio-prospecting of marine-derived fungi with special reference to production of keratinase enzyme - A need-based optimization study

2018

J App Biol Biotech

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Keratin derived from poultry, and other keratinous waste remains a headache to the human society and also to the nature. Marine fungi -an untapped resource to be exploited specifically for the production of keratinase. In this connection, marine sediment samples were randomly collected from Kovalam located along the south west coast of Tamil Nadu. Two marine-derived fungi were isolated from the sediment samples. The internal transcribed spacer region of the isolates was sequenced, submitted to the GenBank and an individual accession number obtained for Colletotrichum capsici (KY817475) and Curvularia lunata (KY828216). Keratinase enzyme was produced with C. capsici and C. lunata at pH 8.5, and total protein was estimated. The amount of protein produced by C. capsici was found to be 0.91 mg/ml and by C. lunata was 1.06 mg/ml. The molecular weight of keratinase was determined as 35kDa. Four parameters through pH, concentration of feather meal, carbon source, and nitrogen source were optimized during the present study. Keratinase production media inoculated with C. capsici and C. lunata exhibit OD max with respect to feather meal concentration and corresponding OD was 1.264 mg/ml and 0.639 mg/ml. The OD max for C. capsici and C. lunata supplemented with maltose as carbon source was 1.281 mg/ml and 1.426 mg/ml. The OD max for C. capsici and C. lunata supplemented with yeast extract as nitrogen source and corresponding OD was 1.858 mg/ml and 1.613 mg/ml. Thus, we conclude that marine-derived fungi C. capsici and C. lunata are potential producers of ecovaluable keratinase which could be used up to treat the ecological burden created by keratinous waste. INTRODUCTIONSoil-borne microfungi are reported to synthesize a wide variety of hydrolytic enzymes. Still several species are exploited in the industries toward the production of industrially important enzymes such as cellulase, pectinase, carbohydrase, and lipase. A unique class of enzyme called keratinase produced by microfungi population has both biotechnological, biomedical potentials, and ecological benefits. Some clinically important pathogenic dermatophytes such as Trichophyton spp.[1] produce this enzyme to invade the skin tissues. On the other hand, the microbial keratinase has ecovaluable role since it is being applied in the bioconversion of poultry and other keratinous waste. For this purpose keratinases trapped from Streptomyces spp. [2,3] and Bacillus spp. [4,5] have been investigated. Around 24 billion chickens were killed annually consequently 8.5 billion tone of poultry feather *Corresponding Author: Samuel Ponpandian, Department of Biotechnology, Ayya Nadar Janaki Ammal College, Sivakasi, Tamil Nadu, India. Email: drsamuelponpandian@rediffmail.com waste are generated. As per a recent citation from India's leading newspaper that our country alone contributes 350 million tone of poultry waste. Discarded feather causes several ailments to human beings such as chlorosis, mycoplasmosis, and fowl cholera [6]. Poultry waste drenched during the ...

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confidence: 61%

Bio-prospecting of marine-derived fungi with special reference to production of keratinase enzyme - A need-based optimization study

2018

J App Biol Biotech

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“…It is useful to identify the important factors and interactions between two or more variables in relatively few experiments as compared to the conventional onefactor-at-time technique (Tumpang et al, 2011). The twolevel factorial design can be considered to be a multivariable sequential search technique in which the effects of two or more factors are studied simultaneously and the responses are analyzed statistically to arrive at a decision (Duta et al, 2006;Anbu et al, 2006). However, this technique is given less attention compared to optimization processes.…”

Section: Ajasmentioning

confidence: 99%

IMPROVED PREDICTION FOR MEDIUM OPTIMIZATION USING FACTORIAL SCREENING FOR DOCOSAHEXAENOIC ACID PRODUCTION BY <i>SCHIZOCHYTRIUM</i> SP. SW1

Manikan¹

2014

American Journal of Applied Sciences

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Docosa Hexaenoic Acid (DHA) is an essential fatty acid that plays major role in human health. It is typically extracted from fatty fish and this puts pressure on global fish stock. DHA obtained from fish is also prone to contamination. Schizochytrium is a potential alternative source, having the ability of accumulating considerable amounts of DHA. Modification of medium components and culture conditions are the preferred techniques to enhance DHA production by this marine microalga. In this study, two-level factorial design was employed to investigate the significance of sea salt, glucose, yeast extract, Monosodium Glutamate (MSG) and peptone in affecting the biomass, lipid and DHA accumulation processes of a locally isolated strain of Schizochytrium. In contrast to the conventional ANOVA method commonly employed in various screening studies, half normal probability plots were used to identify the significant factors, whereas interaction plots were used to explain the pattern of interaction between significant combinations. Design Expert software was used to construct a set of experiments where each medium component mentioned above was varied over two levels. Cultivation was carried out in 250 mL flasks containing 50 mL of medium, incubated at 30°C with 200 rpm agitation for 96 h. This experiment revealed that sea salt and interaction of glucose-MSG have significant effect on biomass production whereas sea salt and interaction of sea salt-glucose affected lipid accumulation. Meanwhile, interactions of glucose-yeast extract and glucose-MSG have significant effect on DHA accumulation. Detailed analysis of interaction plots enabled deeper understanding of the behavior of the system in response to the significant variables, which improves the precision in determination of variable domains for effective medium optimization.

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“…Keratinase assay was performed according to the method of Anbu et al (2007) with minor modifications. Twenty mg (20 mg) of chopped feathers were suspended in 3.8 ml of 100 mM Tris-HCl buffer (pH 8.0) followed by addition of 200 µl of culture supernatant to it.…”

Section: Time Course Study Of Feather Degradation In Basal Feather Mementioning

confidence: 99%

Ameliorating effects of chicken feathers in plant growth promotion activity by a keratinolytic strain of Bacillus subtilis PF1

Bhange

Chaturvedi

Bhatt

2016

Bioresour. Bioprocess.

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Background: Feathers are the major byproducts of poultry industry and considered as waste. Feathers (composed of protein keratin) are metabolized by a number of microorganisms as a source of carbon and nitrogen. Degradation of feathers results in production of amino acids and peptides, which can be employed as precursors for plant growthpromoting metabolites such as indole acetic acid, ammonia and HCN. The aim of the present investigation was to assess the influence of these metabolites (termed as feather protein hydrolysate) on plant growth promotion activity of keratinolytic bacterial strain Bacillus subtilis PF1.Results: Strain PF1 exhibits potent keratinolytic activity and can efficiently degrade 10 g/l chicken feathers under submerged cultivation with 81.4 ± 4.40 U/ml keratinase activity. Different concentrations of feathers supported the production of indole acetic acid by strain PF1. Strain PF1 produces maximum indole acetic acid (46.2 ± 0.21 µg/ml) in the presence of 2.0 % feathers at 120 h of incubation. The indole acetic acid production was confirmed by thin-layer chromatography and Fourier transform infrared spectroscopic analysis. However, increased concentration of feathers exhibited negative effect on phosphate solubilization due to increased alkalinity. HCN production also exhibited positive correlation with concentration of feathers. Finally, plant growth of Vigna radiata in the presence of strain PF1 with chicken feathers in soil was investigated, which showed good plant growth promotion activity. Increased ratio of C/N in soil also supported the plant growth promotion activity of feathers. Conclusion:Feather degradation property of B. subtilis PF1 could be efficiently utilized for feather waste management. The metabolites released by feather degradation along with strain PF1 could be successfully employed as an economic source of nitrogen fertilizers for plants.

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Optimization of extracellular keratinase production by poultry farm isolate Scopulariopsis brevicaulis

Cited by 80 publications

References 20 publications

Bio-prospecting of marine-derived fungi with special reference to production of keratinase enzyme - A need-based optimization study

Bio-prospecting of marine-derived fungi with special reference to production of keratinase enzyme - A need-based optimization study

IMPROVED PREDICTION FOR MEDIUM OPTIMIZATION USING FACTORIAL SCREENING FOR DOCOSAHEXAENOIC ACID PRODUCTION BY <i>SCHIZOCHYTRIUM</i> SP. SW1

Ameliorating effects of chicken feathers in plant growth promotion activity by a keratinolytic strain of Bacillus subtilis PF1

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