Optimization of Enzyme Assisted Extraction of Chondroitin Sulfate from <i>Bohadschia argus</i> by Response Surface Methodology

Ruensodsai, Thanagorn; Panakkal, Elizabeth Jayex; Teerapornnarong, Prawpan; Rodiahwati, Wawat; Sriariyanun, Malinee; Rattanaporn, Kittipong

doi:10.1051/e3sconf/202130202012

Cited by 5 publications

(2 citation statements)

References 26 publications

(18 reference statements)

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“…Ruensodsai et al extracted CS from Bohadschia argu0s and optimized the method using BBD and RSM. The results showed that the CS yield was 4.16 g/100 g dry matter when the papain concentration was 0.48%, the extraction time was 1.01 h, and the extraction temperature was 56.53 °C [29]. Rani et al used papain (1 U/100 mg of tissue) to extract CS with a molecular weight of 100 kDa from chicken breast bones.…”

Section: Extraction Process Optimization Of Cs By Rsm Rsm Canmentioning

confidence: 99%

Extraction, Physicochemical Properties, and In Vitro Antioxidant Activities of Chondroitin Sulfate from Bovine Nose Cartilage

Shi,

Deng,

et al. 2024

International Journal of Food Science

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Beef is an important high-nutrition livestock product, and several byproducts, such as bovine cartilage, are produced during slaughter. To effectively utilize these agricultural and pastoral byproducts, combined (trypsin-papain) enzymolysis and cetylpyridine chloride purification methods were used to obtain chondroitin sulfate (CS) from the nasal cartilage of Shaanxi Yellow cattle. The effects of pH, temperature, and time on the CS yield during enzymatic hydrolysis were investigated, and the CS extraction process was optimized using response surface methodology. The best yield of CS was 21.62% under the optimum conditions of pH 6.51, temperature of 64.53°C, and enzymolysis time of 19.86 h. The molecular weight of CS from Shaanxi cattle nasal cartilage was 89.21 kDa, glucuronic acid content was 31.76±0.72%, protein content was 1.12±0.03%, and sulfate group content was 23.34±0.08%. The nasal cartilage CS of the Yellow cattle showed strong DPPH•, •OH, and ABTS+• radical scavenging abilities and ferrous reduction ability in the experimental concentration range. This study could contribute to “turn waste into treasure” and improve the comprehensive utilization of regional characteristic biological resources.

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Section: Extraction Process Optimization Of Cs By Rsm Rsm Canmentioning

confidence: 99%

Extraction, Physicochemical Properties, and In Vitro Antioxidant Activities of Chondroitin Sulfate from Bovine Nose Cartilage

Shi,

Deng,

et al. 2024

International Journal of Food Science

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“…Therefore, RSM is an effective technique for complex processes. It reduces the cost and time spent on experiments, which makes it easier to manage and explain the results when compared with other methods [24,25,26]. Among the various designs in RSM, Box-Behnken Design was chosen to design the experiment.…”

Section: Rsm Design and Experimental Testingmentioning

confidence: 99%

Oxalic Acid Pretreatment on Enhancement of Enzymatic Saccharification from Napier Grass for Biofuel Production

Panyarachun,

Panakkal,

Tawai

et al. 2023

E3S Web Conf.

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Thailand as an agricultural country faces significant challenges in managing the abundant biomass waste generated from agricultural activities. Conventional disposal methods such as incineration contribute to pollution and limited availability of landfill space. To mitigate these issues valorization of this biomass waste has been a solution. This study focuses on the utilization of Napier grass as a renewable energy source. In this experiment, the Napier grass samples were pretreated using oxalic acid with temperature variations (50 – 100 °C), time (30 180 min), and oxalic acid concentration (2 10%w/v) to determine the limit of these three factors for optimization studies. The utilization of Box-Behnken Design (BBD) within Response Surface Methodology (RSM) enabled the determination of optimal pretreatment conditions and the exploration of the correlation between pretreatment factors and reducing sugar content. The model predicted pretreatment with an oxalic acid concentration of 6% w/v, pretreated at 100 °C for 105 min as the optimal pretreatment condition to produce a maximum reducing sugar concentration of 10.65 mg/ml. Therefore, the sample was pretreated at optimum conditions and the results revealed the amount of reducing sugar obtained was 10.67 mg/ml, which differed from the predicted value with an error of 0.22%. Thus, this study provides insight for future researchers on the optimum condition that can be applied for pretreating biomass with oxalic acid to maximize the sugar yield.

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Optimization of Nutraceuticals Extraction

Malik,

Jabeen,

Anwar

et al. 2024

Methods and Protocols in Food Science

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Optimization of Enzyme Assisted Extraction of Chondroitin Sulfate from Bohadschia argus by Response Surface Methodology

Cited by 5 publications

References 26 publications

Extraction, Physicochemical Properties, and In Vitro Antioxidant Activities of Chondroitin Sulfate from Bovine Nose Cartilage

Extraction, Physicochemical Properties, and In Vitro Antioxidant Activities of Chondroitin Sulfate from Bovine Nose Cartilage

Oxalic Acid Pretreatment on Enhancement of Enzymatic Saccharification from Napier Grass for Biofuel Production

Optimization of Nutraceuticals Extraction

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